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Abstract

Background

Since 2021, an emergence of New Delhi metallo-β-lactamase (NDM)-14-producing has been identified in France. This variant with increased carbapenemase activity was not previously detected in Enterobacterales.

Aim

We investigated the rapid dissemination of NDM-14 producers among patients in hospitals in France.

Methods

All NDM-14-producing non-duplicate clinical isolates identified in France until June 2022 (n = 37) were analysed by whole genome sequencing. The phylogeny of NDM-14-producers among all sequence type (ST) 147 reported in France since 2014 (n = 431) was performed. Antimicrobial susceptibility testing, conjugation experiments, clonal relationship and molecular clock analysis were performed.

Results

The 37 NDM-14 producers recovered in France until 2022 belonged to ST147. The dissemination of NDM-14-producing was linked to a single clone, likely imported from Morocco and responsible for several outbreaks in France. The gene was harboured on a 54 kilobase non-conjugative IncFIB plasmid that shared high homology with a known -carrying plasmid. Using Bayesian analysis, we estimated that the NDM-14-producing ST147 clone appeared in 2020. The evolutionary rate of this clone was estimated to 5.61 single nucleotide polymorphisms per genome per year. The NDM-14 producers were highly resistant to all antimicrobials tested except to colistin, cefiderocol (minimum inhibitory concentration 2 mg/L) and the combination of aztreonam/avibactam.

Conclusion

Highly resistant NDM-14 producing can rapidly spread in healthcare settings. Surveillance and thorough investigations of hospital outbreaks are critical to evaluate and limit the dissemination of this clone.

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2023-10-19
2024-11-25
http://instance.metastore.ingenta.com/content/10.2807/1560-7917.ES.2023.28.42.2300095
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Emergence and rapid dissemination of highly resistant NDM-14-producing Klebsiella pneumoniae ST147, France, 2022
<p class="citation"> <span>Citation style for this article:</span> <span class="meta-value authors"> <a href="/search?value1=C%C3%A9cile+Emeraud&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Emeraud Cécile</a>, <a href="/search?value1=Aba+Mahamat&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Mahamat Aba</a>, <a href="/search?value1=Agn%C3%A8s+B.+Jousset&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Jousset Agnès B.</a>, <a href="/search?value1=Sandrine+Bernabeu&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Bernabeu Sandrine</a>, <a href="/search?value1=Tania+Goncalves&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Goncalves Tania</a>, <a href="/search?value1=Camille+Pommier&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Pommier Camille</a>, <a href="/search?value1=Delphine+Girlich&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Girlich Delphine</a>, <a href="/search?value1=Aur%C3%A9lien+Birer&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Birer Aurélien</a>, <a href="/search?value1=Christophe+Rodriguez&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Rodriguez Christophe</a>, <a href="/search?value1=Jean-Michel+Pawlotsky&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Pawlotsky Jean-Michel</a>, <a href="/search?value1=Thierry+Naas&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Naas Thierry</a>, <a href="/search?value1=R%C3%A9my+A.+Bonnin&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Bonnin Rémy A.</a>, <a href="/search?value1=Laurent+Dortet&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Dortet Laurent</a></span>. Emergence and rapid dissemination of highly resistant NDM-14-producing Klebsiella pneumoniae ST147, France, 2022. <a href="/content/ecdc">Euro Surveill.</a> 2023;28(42):pii=2300095. <a href="https://doi.org/10.2807/1560-7917.ES.2023.28.42.2300095" title="doi link" target="_blank">https://doi.org/10.2807/1560-7917.ES.2023.28.42.2300095</a> <span class="ES_Article_citation"> <span class="generated">Received</span>: 06 Feb 2023;&nbsp;&nbsp; <span class="generated">Accepted</span>: 26 Jun 2023 </span> </p>
/content/10.2807/1560-7917.ES.2023.28.42.2300095
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