- Victor M Corman1, Olfert Landt2, Marco Kaiser2, Richard Molenkamp3, Adam Meijer4, Daniel KW Chu5, Tobias Bleicker1, Sebastian Brünink1, Julia Schneider1, Marie Luisa Schmidt1, Daphne GJC Mulders3, Bart L Haagmans3, Bas van der Veer4, Sharon van den Brink4, Lisa Wijsman4, Gabriel Goderski4, Jean-Louis Romette6, Joanna Ellis7, Maria Zambon7, Malik Peiris5, Herman Goossens8, Chantal Reusken4, Marion PG Koopmans3, Christian Drosten1
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View Affiliations Hide AffiliationsAffiliations: 1 Charité – Universitätsmedizin Berlin Institute of Virology, Berlin, Germany and German Centre for Infection Research (DZIF), Berlin, Germany 2 Tib-Molbiol, Berlin, Germany 3 Department of Viroscience, Erasmus MC, Rotterdam, the Netherlands 4 National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands 5 University of Hong Kong, Hong Kong, China 6 Universite d Aix-Marseille, Marseille, France 7 Public Health England, London, United Kingdom 8 Department of Medical Microbiology, Vaccine and Infectious Diseases Institute, University of Antwerp, Antwerp, BelgiumCorrespondence:Christian Drostenchristian.drosten charite.de
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Citation style for this article: Corman Victor M, Landt Olfert, Kaiser Marco, Molenkamp Richard, Meijer Adam, Chu Daniel KW, Bleicker Tobias, Brünink Sebastian, Schneider Julia, Schmidt Marie Luisa, Mulders Daphne GJC, Haagmans Bart L, van der Veer Bas, van den Brink Sharon, Wijsman Lisa, Goderski Gabriel, Romette Jean-Louis, Ellis Joanna, Zambon Maria, Peiris Malik, Goossens Herman, Reusken Chantal, Koopmans Marion PG, Drosten Christian. Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR. Euro Surveill. 2020;25(3):pii=2000045. https://doi.org/10.2807/1560-7917.ES.2020.25.3.2000045 Received: 21 Jan 2020; Accepted: 22 Jan 2020
Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR
Abstract
The ongoing outbreak of the recently emerged novel coronavirus (2019-nCoV) poses a challenge for public health laboratories as virus isolates are unavailable while there is growing evidence that the outbreak is more widespread than initially thought, and international spread through travellers does already occur.
We aimed to develop and deploy robust diagnostic methodology for use in public health laboratory settings without having virus material available.
Here we present a validated diagnostic workflow for 2019-nCoV, its design relying on close genetic relatedness of 2019-nCoV with SARS coronavirus, making use of synthetic nucleic acid technology.
The workflow reliably detects 2019-nCoV, and further discriminates 2019-nCoV from SARS-CoV. Through coordination between academic and public laboratories, we confirmed assay exclusivity based on 297 original clinical specimens containing a full spectrum of human respiratory viruses. Control material is made available through European Virus Archive – Global (EVAg), a European Union infrastructure project.
The present study demonstrates the enormous response capacity achieved through coordination of academic and public laboratories in national and European research networks.
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References
-
World Health Organization (WHO). Coronavirus. Geneva: WHO; 2020 [Accessed 21 Jan 2020]. Available from: https://www.who.int/health-topics/coronavirus
-
Zhang Y-Z. Novel 2019 coronavirus genome. Virological. [Accessed 21 Jan 2020]. Available from: http://virological.org/t/novel-2019-coronavirus-genome/319
-
de Groot RJ, Baker SC, Baric R, Enjuanes L, Gorbalenya AE, Holmes KV, et al. Family Coronaviridae. In: King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ. Virus taxonomy: classification and nomenclature of viruses: ninth report of the International Committee on Taxonomy of Viruses. London; Waltham: Academic Press; 2012. p. 806-20.
-
Peiris JS, Yuen KY, Osterhaus AD, Stöhr K. The severe acute respiratory syndrome. N Engl J Med. 2003;349(25):2431-41. https://doi.org/10.1056/NEJMra032498 PMID: 14681510
-
World Health Organization. (WHO. Novel Coronavirus (2019-nCoV). Situation report – 1. Geneva: WHO; 21 Jan 2020. Available from: https://www.who.int/docs/default-source/coronaviruse/situation-reports/20200121-sitrep-1-2019-ncov.pdf
-
Abbott A. SARS testing: First past the post. Nature. 2003;423(6936):114. https://doi.org/10.1038/423114a PMID: 12736651
-
Corman VM, Müller MA, Costabel U, Timm J, Binger T, Meyer B, et al. Assays for laboratory confirmation of novel human coronavirus (hCoV-EMC) infections. Euro Surveill. 2012;17(49):20334. https://doi.org/10.2807/ese.17.49.20334-en PMID: 23231891
-
Drosten C, Günther S, Preiser W, van der Werf S, Brodt HR, Becker S, et al. Identification of a novel coronavirus in patients with severe acute respiratory syndrome. N Engl J Med. 2003;348(20):1967-76. https://doi.org/10.1056/NEJMoa030747 PMID: 12690091
-
Corman VM, Eickmann M, Landt O, Bleicker T, Brünink S, Eschbach-Bludau M, et al. Specific detection by real-time reverse-transcription PCR assays of a novel avian influenza A(H7N9) strain associated with human spillover infections in China. Euro Surveill. 2013;18(16):20461. PMID: 23611031
-
Corman VM, Eckerle I, Bleicker T, Zaki A, Landt O, Eschbach-Bludau M, et al. Detection of a novel human coronavirus by real-time reverse-transcription polymerase chain reaction. Euro Surveill. 2012;17(39):20285. https://doi.org/10.2807/ese.17.39.20285-en PMID: 23041020
-
Panning M, Charrel RN, Donoso Mantke O, Landt O, Niedrig M, Drosten C. Coordinated implementation of chikungunya virus reverse transcription-PCR. Emerg Infect Dis. 2009;15(3):469-71. https://doi.org/10.3201/eid1503.081104 PMID: 19239767
-
Corman VM, Rasche A, Baronti C, Aldabbagh S, Cadar D, Reusken CB, et al. Assay optimization for molecular detection of Zika virus. Bull World Health Organ. 2016;94(12):880-92. https://doi.org/10.2471/BLT.16.175950 PMID: 27994281
-
Drexler JF, Gloza-Rausch F, Glende J, Corman VM, Muth D, Goettsche M, et al. Genomic characterization of severe acute respiratory syndrome-related coronavirus in European bats and classification of coronaviruses based on partial RNA-dependent RNA polymerase gene sequences. J Virol. 2010;84(21):11336-49. https://doi.org/10.1128/JVI.00650-10 PMID: 20686038
-
Muth D, Corman VM, Roth H, Binger T, Dijkman R, Gottula LT, et al. Attenuation of replication by a 29 nucleotide deletion in SARS-coronavirus acquired during the early stages of human-to-human transmission. Sci Rep. 2018;8(1):15177. https://doi.org/10.1038/s41598-018-33487-8 PMID: 30310104
-
Corman VM, Muth D, Niemeyer D, Drosten C. Hosts and sources of endemic human coronaviruses. Adv Virus Res. 2018;100:163-88. https://doi.org/10.1016/bs.aivir.2018.01.001 PMID: 29551135
-
Drexler JF, Corman VM, Drosten C. Ecology, evolution and classification of bat coronaviruses in the aftermath of SARS. Antiviral Res. 2014;101:45-56. https://doi.org/10.1016/j.antiviral.2013.10.013 PMID: 24184128
-
Cui J, Li F, Shi ZL. Origin and evolution of pathogenic coronaviruses. Nat Rev Microbiol. 2019;17(3):181-92. https://doi.org/10.1038/s41579-018-0118-9 PMID: 30531947
-
Romette JL, Prat CM, Gould EA, de Lamballerie X, Charrel R, Coutard B, et al. The European Virus Archive goes global: A growing resource for research. Antiviral Res. 2018;158:127-34. https://doi.org/10.1016/j.antiviral.2018.07.017 PMID: 30059721
-
Alleweldt F, Kara S, Osinski A, Van Baal P, Kellerborg K, Aarestrup FM, et al. Developing a framework to assess the costeffectiveness of COMPARE - a global platform for the exchange of sequence-based pathogen data. Rev Sci Tech. 2017;36(1):311-22. https://doi.org/10.20506/rst.36.1.2631 PMID: 28926006
-
Domingo C, Ellerbrok H, Koopmans M, Nitsche A, Leitmeyer K, Charrel RN, et al. Need for additional capacity and improved capability for molecular detection of yellow fever virus in European Expert Laboratories: External Quality Assessment, March 2018. Euro Surveill. 2018;23(28):1800341. https://doi.org/10.2807/1560-7917.ES.2018.23.28.1800341 PMID: 30017021
-
Pas SD, Patel P, Reusken C, Domingo C, Corman VM, Drosten C, et al. First international external quality assessment of molecular diagnostics for Mers-CoV. J Clin Virol. 2015;69:81-5. https://doi.org/10.1016/j.jcv.2015.05.022 PMID: 26209385
-
Gobat N, Amuasi J, Yazdanpanah Y, Sigfid L, Davies H, Byrne JP, et al. Advancing preparedness for clinical research during infectious disease epidemics. ERJ Open Res. 2019;5(2):00227-2018. https://doi.org/10.1183/23120541.00227-2018 PMID: 31123684
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