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Abstract

Background

The ongoing outbreak of the recently emerged novel coronavirus (2019-nCoV) poses a challenge for public health laboratories as virus isolates are unavailable while there is growing evidence that the outbreak is more widespread than initially thought, and international spread through travellers does already occur.

Aim

We aimed to develop and deploy robust diagnostic methodology for use in public health laboratory settings without having virus material available.

Methods

Here we present a validated diagnostic workflow for 2019-nCoV, its design relying on close genetic relatedness of 2019-nCoV with SARS coronavirus, making use of synthetic nucleic acid technology.

Results

The workflow reliably detects 2019-nCoV, and further discriminates 2019-nCoV from SARS-CoV. Through coordination between academic and public laboratories, we confirmed assay exclusivity based on 297 original clinical specimens containing a full spectrum of human respiratory viruses. Control material is made available through European Virus Archive – Global (EVAg), a European Union infrastructure project.

Conclusion

The present study demonstrates the enormous response capacity achieved through coordination of academic and public laboratories in national and European research networks.

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This work is licensed under a Creative Commons Attribution 4.0 International License.
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A corrected article has been published for this content:
Authors’ correction for Euro Surveill. 2020;25(3)
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A corrected article has been published for this content:
Authors’ correction for Euro Surveill. 2020;25(3)
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Addendum for Euro Surveill. 2020;25(3)
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Erratum for Euro Surveill. 2020;25(3)
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/content/10.2807/1560-7917.ES.2020.25.3.2000045
2020-01-23
2024-11-09
http://instance.metastore.ingenta.com/content/10.2807/1560-7917.ES.2020.25.3.2000045
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Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR
<p class="citation"> <span>Citation style for this article:</span> <span class="meta-value authors"> <a href="/search?value1=Victor+M+Corman&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Corman Victor M</a>, <a href="/search?value1=Olfert+Landt&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Landt Olfert</a>, <a href="/search?value1=Marco+Kaiser&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Kaiser Marco</a>, <a href="/search?value1=Richard+Molenkamp&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Molenkamp Richard</a>, <a href="/search?value1=Adam+Meijer&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Meijer Adam</a>, <a href="/search?value1=Daniel+KW+Chu&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Chu Daniel KW</a>, <a href="/search?value1=Tobias+Bleicker&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Bleicker Tobias</a>, <a href="/search?value1=Sebastian+Br%C3%BCnink&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Brünink Sebastian</a>, <a href="/search?value1=Julia+Schneider&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Schneider Julia</a>, <a href="/search?value1=Marie+Luisa+Schmidt&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Schmidt Marie Luisa</a>, <a href="/search?value1=Daphne+GJC+Mulders&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Mulders Daphne GJC</a>, <a href="/search?value1=Bart+L+Haagmans&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Haagmans Bart L</a>, <a href="/search?value1=Bas+van+der+Veer&option1=author&noRedirect=true" class="nonDisambigAuthorLink">van der Veer Bas</a>, <a href="/search?value1=Sharon+van+den+Brink&option1=author&noRedirect=true" class="nonDisambigAuthorLink">van den Brink Sharon</a>, <a href="/search?value1=Lisa+Wijsman&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Wijsman Lisa</a>, <a href="/search?value1=Gabriel+Goderski&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Goderski Gabriel</a>, <a href="/search?value1=Jean-Louis+Romette&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Romette Jean-Louis</a>, <a href="/search?value1=Joanna+Ellis&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Ellis Joanna</a>, <a href="/search?value1=Maria+Zambon&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Zambon Maria</a>, <a href="/search?value1=Malik+Peiris&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Peiris Malik</a>, <a href="/search?value1=Herman+Goossens&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Goossens Herman</a>, <a href="/search?value1=Chantal+Reusken&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Reusken Chantal</a>, <a href="/search?value1=Marion+PG+Koopmans&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Koopmans Marion PG</a>, <a href="/search?value1=Christian+Drosten&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Drosten Christian</a></span>. Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR. <a href="/content/ecdc">Euro Surveill.</a> 2020;25(3):pii=2000045. <a href="https://doi.org/10.2807/1560-7917.ES.2020.25.3.2000045" title="doi link" target="_blank">https://doi.org/10.2807/1560-7917.ES.2020.25.3.2000045</a> <span class="ES_Article_citation"> <span class="generated">Received</span>: 21 Jan 2020;&nbsp;&nbsp; <span class="generated">Accepted</span>: 22 Jan 2020 </span> </p>
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