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Abstract

Genomic surveillance during ebolavirus outbreaks to elucidate transmission chains and develop diagnostic tests is delayed by the laborious development of variant-specific laboratory assays. We developed a new protocol combining 31 parallel PCR assays with Illumina/MinION-based sequencing, allowing generic ebolavirus genomic surveillance, validated using cell culture-derived Ebola, Reston, Sudan and Taï Forest virus at concentrations compatible with patient viral loads. Our approach enables pre-emptive genomic surveillance of ongoing and future ebolavirus outbreaks irrespective of variant divergence.

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/content/10.2807/1560-7917.ES.2020.25.3.1900765
2020-01-23
2024-11-21
http://instance.metastore.ingenta.com/content/10.2807/1560-7917.ES.2020.25.3.1900765
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Pre-emptive genomic surveillance of emerging ebolaviruses
<p class="citation"> <span>Citation style for this article:</span> <span class="meta-value authors"> <a href="/search?value1=Ignacio+Postigo-Hidalgo&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Postigo-Hidalgo Ignacio</a>, <a href="/search?value1=Carlo+Fischer&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Fischer Carlo</a>, <a href="/search?value1=Andres+Moreira-Soto&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Moreira-Soto Andres</a>, <a href="/search?value1=Patricia+Tscheak&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Tscheak Patricia</a>, <a href="/search?value1=Michael+Nagel&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Nagel Michael</a>, <a href="/search?value1=Markus+Eickmann&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Eickmann Markus</a>, <a href="/search?value1=Jan+Felix+Drexler&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Drexler Jan Felix</a></span>. Pre-emptive genomic surveillance of emerging ebolaviruses. <a href="/content/ecdc">Euro Surveill.</a> 2020;25(3):pii=1900765. <a href="https://doi.org/10.2807/1560-7917.ES.2020.25.3.1900765" title="doi link" target="_blank">https://doi.org/10.2807/1560-7917.ES.2020.25.3.1900765</a> <span class="ES_Article_citation"> <span class="generated">Received</span>: 20 Dec 2019;&nbsp;&nbsp; <span class="generated">Accepted</span>: 22 Jan 2020 </span> </p>
/content/10.2807/1560-7917.ES.2020.25.3.1900765
/content/10.2807/1560-7917.ES.2020.25.3.1900765
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