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Abstract

We investigated why a clinical meticillin-resistant (MRSA) isolate yielded false-negative results with some commercial PCR tests for MRSA detection. We found that an epidemic European CC1-MRSA-IV clone generally exhibits this behaviour. The failure of the assays was attributable to a large insertion in the /SCC integration site. To ensure the reliability of molecular MRSA tests, it is vital to monitor emergence of new SCC types and junction sites.

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/content/10.2807/1560-7917.ES.2020.25.25.2000929
2020-06-25
2024-11-21
http://instance.metastore.ingenta.com/content/10.2807/1560-7917.ES.2020.25.25.2000929
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An epidemic CC1-MRSA-IV clone yields false-negative test results in molecular MRSA identification assays: a note of caution, Austria, Germany, Ireland, 2020
<p class="citation"> <span>Citation style for this article:</span> <span class="meta-value authors"> <a href="/search?value1=Stefan+Monecke&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Monecke Stefan</a>, <a href="/search?value1=Elisabeth+K%C3%B6nig&option1=author&noRedirect=true" class="nonDisambigAuthorLink">König Elisabeth</a>, <a href="/search?value1=Megan+R+Earls&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Earls Megan R</a>, <a href="/search?value1=Eva+Leitner&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Leitner Eva</a>, <a href="/search?value1=Elke+M%C3%BCller&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Müller Elke</a>, <a href="/search?value1=Gabriel+E+Wagner&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Wagner Gabriel E</a>, <a href="/search?value1=David+M+Poitz&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Poitz David M</a>, <a href="/search?value1=Lutz+Jatzwauk&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Jatzwauk Lutz</a>, <a href="/search?value1=Teodora+Vremer%C7%8E&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Vremerǎ Teodora</a>, <a href="/search?value1=Olivia+S+Dorneanu&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Dorneanu Olivia S</a>, <a href="/search?value1=Alexandra+Simbeck&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Simbeck Alexandra</a>, <a href="/search?value1=Andreas+Ambrosch&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Ambrosch Andreas</a>, <a href="/search?value1=Ines+Zollner-Schwetz&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Zollner-Schwetz Ines</a>, <a href="/search?value1=Robert+Krause&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Krause Robert</a>, <a href="/search?value1=Werner+Ruppitsch&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Ruppitsch Werner</a>, <a href="/search?value1=Wulf+Schneider-Brachert&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Schneider-Brachert Wulf</a>, <a href="/search?value1=David+C+Coleman&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Coleman David C</a>, <a href="/search?value1=Ivo+Steinmetz&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Steinmetz Ivo</a>, <a href="/search?value1=Ralf+Ehricht&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Ehricht Ralf</a></span>. An epidemic CC1-MRSA-IV clone yields false-negative test results in molecular MRSA identification assays: a note of caution, Austria, Germany, Ireland, 2020. <a href="/content/ecdc">Euro Surveill.</a> 2020;25(25):pii=2000929. <a href="https://doi.org/10.2807/1560-7917.ES.2020.25.25.2000929" title="doi link" target="_blank">https://doi.org/10.2807/1560-7917.ES.2020.25.25.2000929</a> <span class="ES_Article_citation"> <span class="generated">Received</span>: 14 May 2020;&nbsp;&nbsp; <span class="generated">Accepted</span>: 18 Jun 2020 </span> </p>
/content/10.2807/1560-7917.ES.2020.25.25.2000929
/content/10.2807/1560-7917.ES.2020.25.25.2000929
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