1887
  1. Home
  2. Eurosurveillance
  3. Volume 25, Issue 25, 25/Jun/2020
  4. Article
Rapid communication Open Access
Like 0
Download

Abstract

We investigated why a clinical meticillin-resistant (MRSA) isolate yielded false-negative results with some commercial PCR tests for MRSA detection. We found that an epidemic European CC1-MRSA-IV clone generally exhibits this behaviour. The failure of the assays was attributable to a large insertion in the /SCC integration site. To ensure the reliability of molecular MRSA tests, it is vital to monitor emergence of new SCC types and junction sites.

© Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Loading

Article metrics loading...

/content/10.2807/1560-7917.ES.2020.25.25.2000929
2020-06-25
2024-11-16
http://instance.metastore.ingenta.com/content/10.2807/1560-7917.ES.2020.25.25.2000929
Loading
Loading full text...

Full text loading...

/deliver/fulltext/eurosurveillance/25/25/eurosurv-25-25-3.html?itemId=/content/10.2807/1560-7917.ES.2020.25.25.2000929&mimeType=html&fmt=ahah

References

  1. Earls MR, Shore AC, Brennan GI, Simbeck A, Schneider-Brachert W, Vremerǎ T, et al. A novel multidrug-resistant PVL-negative CC1-MRSA-IV clone emerging in Ireland and Germany likely originated in South-Eastern Europe. Infect Genet Evol. 2019;69:117-26.  https://doi.org/10.1016/j.meegid.2019.01.021  PMID: 30677533 
  2. The European Committee on Antimicrobial Susceptibility Testing (EUCAST). EUCAST rapid antimicrobial susceptibility testing (RAST) directly from positive blood culture bottles. Version 1.1. Växjö: EUCAST; 2019. Available from: https://www.eucast.org/fileadmin/src/media/PDFs/EUCAST_files/RAST/EUCAST_RAST_methodology_v1.1_Final.pdf
  3. The European Committee on Antimicrobial Susceptibility Testing (EUCAST). Zone diameter breakpoints for rapid antimicrobial susceptibility testing (RAST) directly from blood culture bottles. Version 1.1. Växjö: EUCAST; 2019. Available from: https://www.eucast.org/fileadmin/src/media/PDFs/EUCAST_files/RAST/EUCAST_RAST_Breakpoint_Table_v_1.1_final_PDF.pdf
  4. Monecke S, Müller E, Dorneanu OS, Vremeră T, Ehricht R. Molecular typing of MRSA and of clinical Staphylococcus aureus isolates from Iaşi, Romania. PLoS One. 2014;9(5):e97833.  https://doi.org/10.1371/journal.pone.0097833  PMID: 24846009 
  5. Monecke S, Coombs G, Shore AC, Coleman DC, Akpaka P, Borg M, et al. A field guide to pandemic, epidemic and sporadic clones of methicillin-resistant Staphylococcus aureus. PLoS One. 2011;6(4):e17936.  https://doi.org/10.1371/journal.pone.0017936  PMID: 21494333 
  6. Earls MR, Coleman DC, Brennan GI, Fleming T, Monecke S, Slickers P, et al. Intra-hospital, inter-hospital and intercontinental spread of ST78 MRSA from two neonatal intensive care unit outbreaks established using whole-genome sequencing. Front Microbiol. 2018;9(1485):1485.  https://doi.org/10.3389/fmicb.2018.01485  PMID: 30022976 
  7. Chen S, Zhou Y, Chen Y, Gu J. fastp: an ultra-fast all-in-one FASTQ preprocessor. Bioinformatics. 2018;34(17):i884-90.  https://doi.org/10.1093/bioinformatics/bty560  PMID: 30423086 
  8. Bankevich A, Nurk S, Antipov D, Gurevich AA, Dvorkin M, Kulikov AS, et al. SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing. J Comput Biol. 2012;19(5):455-77.  https://doi.org/10.1089/cmb.2012.0021  PMID: 22506599 
  9. Earls MR, Kinnevey PM, Brennan GI, Lazaris A, Skally M, O’Connell B, et al. The recent emergence in hospitals of multidrug-resistant community-associated sequence type 1 and spa type t127 methicillin-resistant Staphylococcus aureus investigated by whole-genome sequencing: Implications for screening. PLoS One. 2017;12(4):e0175542.  https://doi.org/10.1371/journal.pone.0175542  PMID: 28399151 
  10. Manara S, Pasolli E, Dolce D, Ravenni N, Campana S, Armanini F, et al. Whole-genome epidemiology, characterisation, and phylogenetic reconstruction of Staphylococcus aureus strains in a paediatric hospital. Genome Med. 2018;10(1):82.  https://doi.org/10.1186/s13073-018-0593-7  PMID: 30424799 
  11. Scheithauer S, Trepels-Kottek S, Häfner H, Keller D, Ittel T, Wagner N, et al. Healthcare worker-related MRSA cluster in a German neonatology level III ICU: a true European story. Int J Hyg Environ Health. 2014;217(2-3):307-11.  https://doi.org/10.1016/j.ijheh.2013.07.006  PMID: 23953255 
  12. Monecke S, Jatzwauk L, Müller E, Nitschke H, Pfohl K, Slickers P, et al. Diversity of SCCmec elements in Staphylococcus aureus as observed in south-eastern Germany. PLoS One. 2016;11(9):e0162654.  https://doi.org/10.1371/journal.pone.0162654  PMID: 27648947 
  13. Loncaric I, Künzel F, Licka T, Simhofer H, Spergser J, Rosengarten R. Identification and characterization of methicillin-resistant Staphylococcus aureus (MRSA) from Austrian companion animals and horses. Vet Microbiol. 2014;168(2-4):381-7.  https://doi.org/10.1016/j.vetmic.2013.11.022  PMID: 24332703 
  14. Loncaric I, Stalder GL, Mehinagic K, Rosengarten R, Hoelzl F, Knauer F, et al. Comparison of ESBL--and AmpC producing Enterobacteriaceae and methicillin-resistant Staphylococcus aureus (MRSA) isolated from migratory and resident population of rooks (Corvus frugilegus) in Austria. PLoS One. 2013;8(12):e84048.  https://doi.org/10.1371/journal.pone.0084048  PMID: 24391878 
  15. Alba P, Feltrin F, Cordaro G, Porrero MC, Kraushaar B, Argudín MA, et al. Livestock-associated methicillin resistant and methicillin susceptible Staphylococcus aureus sequence type (CC)1 in european farmed animals: high genetic relatedness of isolates from Italian cattle herds and humans. PLoS One. 2015;10(8):e0137143.  https://doi.org/10.1371/journal.pone.0137143  PMID: 26322785 
  16. Baig S, Johannesen TB, Overballe-Petersen S, Larsen J, Larsen AR, Stegger M. Novel SCCmec type XIII (9A) identified in an ST152 methicillin-resistant Staphylococcus aureus. Infect Genet Evol. 2018;61:74-6.  https://doi.org/10.1016/j.meegid.2018.03.013  PMID: 29567305 
  17. Bartels MD, Boye K, Rohde SM, Larsen AR, Torfs H, Bouchy P, et al. A common variant of staphylococcal cassette chromosome mec type IVa in isolates from Copenhagen, Denmark, is not detected by the BD GeneOhm methicillin-resistant Staphylococcus aureus assay. J Clin Microbiol. 2009;47(5):1524-7.  https://doi.org/10.1128/JCM.02153-08  PMID: 19297600 
  18. Bartels MD, Hansen LH, Boye K, Sørensen SJ, Westh H. An unexpected location of the arginine catabolic mobile element (ACME) in a USA300-related MRSA strain. PLoS One. 2011;6(1):e16193.  https://doi.org/10.1371/journal.pone.0016193  PMID: 21283578 
  19. Hill-Cawthorne GA, Hudson LO, El Ghany MFA, Piepenburg O, Nair M, Dodgson A, et al. Recombinations in staphylococcal cassette chromosome mec elements compromise the molecular detection of methicillin resistance in Staphylococcus aureus. PLoS One. 2014;9(6):e101419.  https://doi.org/10.1371/journal.pone.0101419  PMID: 24972080 
An epidemic CC1-MRSA-IV clone yields false-negative test results in molecular MRSA identification assays: a note of caution, Austria, Germany, Ireland, 2020
<p class="citation"> <span>Citation style for this article:</span> <span class="meta-value authors"> <a href="/search?value1=Stefan+Monecke&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Monecke Stefan</a>, <a href="/search?value1=Elisabeth+K%C3%B6nig&option1=author&noRedirect=true" class="nonDisambigAuthorLink">König Elisabeth</a>, <a href="/search?value1=Megan+R+Earls&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Earls Megan R</a>, <a href="/search?value1=Eva+Leitner&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Leitner Eva</a>, <a href="/search?value1=Elke+M%C3%BCller&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Müller Elke</a>, <a href="/search?value1=Gabriel+E+Wagner&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Wagner Gabriel E</a>, <a href="/search?value1=David+M+Poitz&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Poitz David M</a>, <a href="/search?value1=Lutz+Jatzwauk&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Jatzwauk Lutz</a>, <a href="/search?value1=Teodora+Vremer%C7%8E&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Vremerǎ Teodora</a>, <a href="/search?value1=Olivia+S+Dorneanu&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Dorneanu Olivia S</a>, <a href="/search?value1=Alexandra+Simbeck&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Simbeck Alexandra</a>, <a href="/search?value1=Andreas+Ambrosch&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Ambrosch Andreas</a>, <a href="/search?value1=Ines+Zollner-Schwetz&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Zollner-Schwetz Ines</a>, <a href="/search?value1=Robert+Krause&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Krause Robert</a>, <a href="/search?value1=Werner+Ruppitsch&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Ruppitsch Werner</a>, <a href="/search?value1=Wulf+Schneider-Brachert&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Schneider-Brachert Wulf</a>, <a href="/search?value1=David+C+Coleman&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Coleman David C</a>, <a href="/search?value1=Ivo+Steinmetz&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Steinmetz Ivo</a>, <a href="/search?value1=Ralf+Ehricht&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Ehricht Ralf</a></span>. An epidemic CC1-MRSA-IV clone yields false-negative test results in molecular MRSA identification assays: a note of caution, Austria, Germany, Ireland, 2020. <a href="/content/ecdc">Euro Surveill.</a> 2020;25(25):pii=2000929. <a href="https://doi.org/10.2807/1560-7917.ES.2020.25.25.2000929" title="doi link" target="_blank">https://doi.org/10.2807/1560-7917.ES.2020.25.25.2000929</a> <span class="ES_Article_citation"> <span class="generated">Received</span>: 14 May 2020;&nbsp;&nbsp; <span class="generated">Accepted</span>: 18 Jun 2020 </span> </p>
/content/10.2807/1560-7917.ES.2020.25.25.2000929
/content/10.2807/1560-7917.ES.2020.25.25.2000929
Loading

Data & Media loading...

Supplementary data

Submit comment
Close
Comment moderation successfully completed
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error