Specific detection by real-time reverse-transcription PCR assays of a novel avian influenza A(H7N9) strain associated with human spillover infections in China

V M Corman; M Eickmann; O Landt; T Bleicker; S Brünink; M Eschbach-Bludau; M Matrosovich; S Becker; C Drosten

doi:10.2807/ese.18.16.20461-en

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Specific detection by real-time reverse-transcription PCR assays of a novel avian influenza A(H7N9) strain associated with human spillover infections in China
V M Corman¹ , M Eickmann² , O Landt³ , T Bleicker¹ , S Brünink¹ , M Eschbach-Bludau¹ , M Matrosovich² , S Becker² , C Drosten¹
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Affiliations: ¹ Institute of Virology, University of Bonn Medical Centre, Bonn, Germany ² Institute for Virology, University of Marburg, Marburg, Germany ³ TibMolbiol, Berlin, Germany

Correspondence:
C Drosten
drosten virology-bonn.de
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Citation style for this article: Corman V M, Eickmann M, Landt O, Bleicker T, Brünink S, Eschbach-Bludau M, Matrosovich M, Becker S, Drosten C. Specific detection by real-time reverse-transcription PCR assays of a novel avian influenza A(H7N9) strain associated with human spillover infections in China. Euro Surveill. 2013;18(16):pii=20461. https://doi.org/10.2807/ese.18.16.20461-en Received: 12 Apr 2013

Abstract

In response to a recent outbreak in China, detection assays for a novel avian influenza A(H7N9) virus need to be implemented in a large number of public health laboratories. Here we present real-time reverse-transcription polymerase chain reaction (RT-PCR) assays for specific detection of this virus, along with clinical validation data and biologically-safe positive controls.