Assays for laboratory confirmation of novel human coronavirus (hCoV-EMC) infections

V M Corman; M A Müller; U Costabel; J Timm; T Binger; B Meyer; P Kreher; E Lattwein; M Eschbach-Bludau; A Nitsche; T Bleicker; O Landt; B Schweiger; J F Drexler; A D Osterhaus; B L Haagmans; U Dittmer; F Bonin; T Wolff; C Drosten

doi:10.2807/ese.17.49.20334-en

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Assays for laboratory confirmation of novel human coronavirus (hCoV-EMC) infections
V M Corman^1,2 , M A Müller^1,2 , U Costabel³ , J Timm⁴ , T Binger¹ , B Meyer¹ , P Kreher⁵ , E Lattwein⁶ , M Eschbach-Bludau¹ , A Nitsche⁵ , T Bleicker¹ , O Landt⁷ , B Schweiger⁵ , J F Drexler¹ , A D Osterhaus⁸ , B L Haagmans⁸ , U Dittmer⁴ , F Bonin³ , T Wolff⁵ , C Drosten¹
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Affiliations: ¹ Institute of Virology, University of Bonn Medical Centre, Bonn, Germany ² These authors contributed equally to this work ³ Ruhrlandklinik, University of Duisburg-Essen, Essen, Germany ⁴ Institute of Virology, University of Duisburg-Essen, Essen, Germany ⁵ Robert Koch Institute, Berlin, Germany ⁶ Euroimmun AG, Lübeck, Germany ⁷ TibMolbiol, Berlin, Germany ⁸ Virosciences Laboratory, Erasmus MC, Rotterdam, the Netherlands

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C Drosten
drosten virology-bonn.de
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Citation style for this article: Corman V M, Müller M A, Costabel U, Timm J, Binger T, Meyer B, Kreher P, Lattwein E, Eschbach-Bludau M, Nitsche A, Bleicker T, Landt O, Schweiger B, Drexler J F, Osterhaus A D, Haagmans B L, Dittmer U, Bonin F, Wolff T, Drosten C. Assays for laboratory confirmation of novel human coronavirus (hCoV-EMC) infections. Euro Surveill. 2012;17(49):pii=20334. https://doi.org/10.2807/ese.17.49.20334-en Received: 05 Dec 2012

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Abstract

We present a rigorously validated and highly sensitive confirmatory real-time RT-PCR assay (1A assay) that can be used in combination with the previously reported upE assay. Two additional RT-PCR assays for sequencing are described, targeting the RdRp gene (RdRpSeq assay) and N gene (NSeq assay), where an insertion/deletion polymorphism might exist among different hCoV-EMC strains. Finally, a simplified and biologically safe protocol for detection of antibody response by immunofluorescence microscopy was developed using convalescent patient serum.