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Abstract

Monkeypox virus (MPXV) is an emerging zoonotic pathogen with complex epidemiology necessitating rapid diagnosis and distinguishing between clades and subclades. The emerging Clade Ib lacks the genomic region used in the Clade I-specific assay from the Centers for Disease Control and Prevention. We report an MPXV real-time PCR to specifically detect Clade Ib. The assay demonstrated proficient sensitivity and specificity in 92 samples and can be included along other TaqMan-based assays to detect MPXV and distinguish between clades and subclades.

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/content/10.2807/1560-7917.ES.2024.29.32.2400486
2024-08-08
2024-08-12
http://instance.metastore.ingenta.com/content/10.2807/1560-7917.ES.2024.29.32.2400486
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Real-time PCR assay to detect the novel Clade Ib monkeypox virus, September 2023 to May 2024
<p class="citation"> <span>Citation style for this article:</span> <span class="meta-value authors"> <a href="/search?value1=Leonard+Schuele&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Schuele Leonard</a>, <a href="/search?value1=Leandre+Murhula+Masirika&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Masirika Leandre Murhula</a>, <a href="/search?value1=Jean+Claude+Udahemuka&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Udahemuka Jean Claude</a>, <a href="/search?value1=Freddy+Belesi+Siangoli&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Siangoli Freddy Belesi</a>, <a href="/search?value1=Justin+Bengehya+Mbiribindi&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Mbiribindi Justin Bengehya</a>, <a href="/search?value1=Pacifique+Ndishimye&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Ndishimye Pacifique</a>, <a href="/search?value1=Frank+M+Aarestrup&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Aarestrup Frank M</a>, <a href="/search?value1=Marion+Koopmans&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Koopmans Marion</a>, <a href="/search?value1=Bas+B+Oude+Munnink&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Oude Munnink Bas B</a>, <a href="/search?value1=Richard+Molenkamp&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Molenkamp Richard</a>, <a href="/search?value1=GREATLIFE+MPOX+group&option1=author&noRedirect=true" class="nonDisambigAuthorLink">GREATLIFE MPOX group</a></span>. Real-time PCR assay to detect the novel Clade Ib monkeypox virus, September 2023 to May 2024. <a href="/content/ecdc">Euro Surveill.</a> 2024;29(32):pii=2400486. <a href="https://doi.org/10.2807/1560-7917.ES.2024.29.32.2400486" title="doi link" target="_blank">https://doi.org/10.2807/1560-7917.ES.2024.29.32.2400486</a> <span class="ES_Article_citation"> <span class="generated">Received</span>: 23 Jul 2024;&nbsp;&nbsp; <span class="generated">Accepted</span>: 07 Aug 2024 </span> </p>
/content/10.2807/1560-7917.ES.2024.29.32.2400486
/content/10.2807/1560-7917.ES.2024.29.32.2400486
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