1887
  1. Home
  2. Eurosurveillance
  3. Volume 29, Issue 28, 11/Jul/2024
  4. Article
Rapid communication Open Access
Like 0
Download

Abstract

We investigated a variant of measles virus that encodes three mismatches to the reverse priming site for a widely used diagnostic real-time RT-PCR assay; reduction of sensitivity was hypothesised. We examined performance of the assay in context of the variant using in silico data, synthetic RNA templates and clinical specimens. Sensitivity was reduced observed at low copy numbers for templates encoding the variant sequence. We designed and tested an alternate priming strategy, rescuing the sensitivity of the assay.

© Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Loading

Article metrics loading...

/content/10.2807/1560-7917.ES.2024.29.28.2400410
2024-07-11
2024-07-15
http://instance.metastore.ingenta.com/content/10.2807/1560-7917.ES.2024.29.28.2400410
Loading
Loading full text...

Full text loading...

/deliver/fulltext/eurosurveillance/29/28/eurosurv-29-28-1_BECK.html?itemId=/content/10.2807/1560-7917.ES.2024.29.28.2400410&mimeType=html&fmt=ahah

References

  1. Hummel KB, Lowe L, Bellini WJ, Rota PA. Development of quantitative gene-specific real-time RT-PCR assays for the detection of measles virus in clinical specimens. J Virol Methods. 2006;132(1-2):166-73.  https://doi.org/10.1016/j.jviromet.2005.10.006  PMID: 16274752 
  2. Pérez-Rodríguez FJ, Cherpillod P, Thomasson V, Vetter P, Schibler M. Identification of a measles variant displaying mutations impacting molecular diagnostics, Geneva, Switzerland, 2023. Euro Surveill. 2024;29(5):2400034.  https://doi.org/10.2807/1560-7917.ES.2024.29.5.2400034  PMID: 38304951 
  3. Fappani C, Gori M, Bianchi S, Canuti M, Colzani D, Baggieri M, et al. Letter to the editor: Further identification of a measles variant displaying mutations impacting molecular diagnostics, Northern Italy, 2024. Euro Surveill. 2024;29(7):2400079.  https://doi.org/10.2807/1560-7917.ES.2023.29.7.2400079  PMID: 38362623 
  4. Genetic diversity of wild-type measles viruses and the global measles nucleotide surveillance database (MeaNS). Wkly Epidemiol Rec. 2015;90(30):373-80. PMID: 26211016 
  5. Cock PJ, Antao T, Chang JT, Chapman BA, Cox CJ, Dalke A, et al. Biopython: freely available Python tools for computational molecular biology and bioinformatics. Bioinformatics. 2009;25(11):1422-3.  https://doi.org/10.1093/bioinformatics/btp163  PMID: 19304878 
  6. Zubach V, Severini A, Hiebert J. Development of a rapid, internally controlled, two target, real-time RT-PCR for detection of measles virus. J Virol Methods. 2022;299:114349.  https://doi.org/10.1016/j.jviromet.2021.114349  PMID: 34740707 
  7. Gardy JL, Naus M, Amlani A, Chung W, Kim H, Tan M, et al. Whole-genome sequencing of measles virus genotypes H1 and D8 during outbreaks of infection following the 2010 Olympic winter games reveals viral transmission routes. J Infect Dis. 2015;212(10):1574-8.  https://doi.org/10.1093/infdis/jiv271  PMID: 26153409 
  8. Masters NB, Beck AS, Mathis AD, Leung J, Raines K, Paul P, et al. Measles virus transmission patterns and public health responses during Operation Allies Welcome: a descriptive epidemiological study. Lancet Public Health. 2023;8(8):e618-28.  https://doi.org/10.1016/S2468-2667(23)00130-5  PMID: 37516478 
Evaluation of the sensitivity of a measles diagnostic real-time RT-PCR assay incorporating recently observed priming mismatch variants, 2024
<p class="citation"> <span>Citation style for this article:</span> <span class="meta-value authors"> <a href="/search?value1=Andrew+S+Beck&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Beck Andrew S</a>, <a href="/search?value1=Elena+N+Lopareva&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Lopareva Elena N</a>, <a href="/search?value1=Hyun+Hwang&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Hwang Hyun</a>, <a href="/search?value1=Derek+Hart&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Hart Derek</a>, <a href="/search?value1=Marcos+de+Almeida&option1=author&noRedirect=true" class="nonDisambigAuthorLink">de Almeida Marcos</a>, <a href="/search?value1=Raydel+Anderson&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Anderson Raydel</a>, <a href="/search?value1=Paul+A+Rota&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Rota Paul A</a>, <a href="/search?value1=Bettina+Bankamp&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Bankamp Bettina</a></span>. Evaluation of the sensitivity of a measles diagnostic real-time RT-PCR assay incorporating recently observed priming mismatch variants, 2024. <a href="/content/ecdc">Euro Surveill.</a> 2024;29(28):pii=2400410. <a href="https://doi.org/10.2807/1560-7917.ES.2024.29.28.2400410" title="doi link" target="_blank">https://doi.org/10.2807/1560-7917.ES.2024.29.28.2400410</a> <span class="ES_Article_citation"> <span class="generated">Received</span>: 25 Jun 2024;&nbsp;&nbsp; <span class="generated">Accepted</span>: 10 Jul 2024 </span> </p>
/content/10.2807/1560-7917.ES.2024.29.28.2400410
/content/10.2807/1560-7917.ES.2024.29.28.2400410
Loading

Data & Media loading...

Supplementary data

Submit comment
Close
Comment moderation successfully completed
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error