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Abstract

We investigated a variant of measles virus that encodes three mismatches to the reverse priming site for a widely used diagnostic real-time RT-PCR assay; reduction of sensitivity was hypothesised. We examined performance of the assay in context of the variant using in silico data, synthetic RNA templates and clinical specimens. Sensitivity was reduced observed at low copy numbers for templates encoding the variant sequence. We designed and tested an alternate priming strategy, rescuing the sensitivity of the assay.

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/content/10.2807/1560-7917.ES.2024.29.28.2400410
2024-07-11
2024-11-22
http://instance.metastore.ingenta.com/content/10.2807/1560-7917.ES.2024.29.28.2400410
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Evaluation of the sensitivity of a measles diagnostic real-time RT-PCR assay incorporating recently observed priming mismatch variants, 2024
<p class="citation"> <span>Citation style for this article:</span> <span class="meta-value authors"> <a href="/search?value1=Andrew+S+Beck&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Beck Andrew S</a>, <a href="/search?value1=Elena+N+Lopareva&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Lopareva Elena N</a>, <a href="/search?value1=Hyun+Hwang&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Hwang Hyun</a>, <a href="/search?value1=Derek+Hart&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Hart Derek</a>, <a href="/search?value1=Marcos+de+Almeida&option1=author&noRedirect=true" class="nonDisambigAuthorLink">de Almeida Marcos</a>, <a href="/search?value1=Raydel+Anderson&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Anderson Raydel</a>, <a href="/search?value1=Paul+A+Rota&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Rota Paul A</a>, <a href="/search?value1=Bettina+Bankamp&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Bankamp Bettina</a></span>. Evaluation of the sensitivity of a measles diagnostic real-time RT-PCR assay incorporating recently observed priming mismatch variants, 2024. <a href="/content/ecdc">Euro Surveill.</a> 2024;29(28):pii=2400410. <a href="https://doi.org/10.2807/1560-7917.ES.2024.29.28.2400410" title="doi link" target="_blank">https://doi.org/10.2807/1560-7917.ES.2024.29.28.2400410</a> <span class="ES_Article_citation"> <span class="generated">Received</span>: 25 Jun 2024;&nbsp;&nbsp; <span class="generated">Accepted</span>: 10 Jul 2024 </span> </p>
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