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Abstract

Background

Carbapenemase-producing Enterobacterales (CPE) are rapidly increasing worldwide, also in Europe. Although prevalence of CPE in Germany is comparatively low, the National Reference Centre for Multidrug-resistant Gram-negative Bacteria noted annually increasing numbers of NDM-5-producing isolates.

Aim

As part of our ongoing surveillance programme, we characterised NDM-5-producing isolates received between 2013 and 2019 using whole genome sequencing (WGS).

Methods

From 329 identified NDM-5-producing , 224 isolates from known geographical locations were subjected to Illumina WGS. Analyses of 222 sequenced isolates included multilocus sequence typing (MLST), core genome (cg)MLST and single-nucleotide polymorphism (SNP)-based analyses.

Results

Results of cgMLST revealed genetically distinct clusters for many of the 43 detected sequence types (ST), of which ST167, ST410, ST405 and ST361 predominated. The SNP-based phylogenetic analyses combined with geographical information identified sporadic cases of nosocomial transmission on a small spatial scale. However, we identified large clusters corresponding to clonal dissemination of ST167, ST410, ST405 and ST361 strains in consecutive years in different regions in Germany.

Conclusion

Occurrence of NDM-5-producing rose in Germany, which was to a large extent due to the increased prevalence of isolates belonging to the international high-risk clones ST167, ST410, ST405 and ST361. Of particular concern is the supra-regional dissemination of these epidemic clones. Available information suggest community spread of NDM-5-producing in Germany, highlighting the importance of epidemiological investigation and an integrated surveillance system in the One Health framework.

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This work is licensed under a Creative Commons Attribution 4.0 International License.
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2023-03-09
2024-11-29
http://instance.metastore.ingenta.com/content/10.2807/1560-7917.ES.2023.28.10.2200509
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Molecular surveillance reveals the emergence and dissemination of NDM-5-producing Escherichia coli high-risk clones in Germany, 2013 to 2019
<p class="citation"> <span>Citation style for this article:</span> <span class="meta-value authors"> <a href="/search?value1=J%C3%B6rg+B+Hans&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Hans Jörg B</a>, <a href="/search?value1=Niels+Pfennigwerth&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Pfennigwerth Niels</a>, <a href="/search?value1=Bernd+Neumann&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Neumann Bernd</a>, <a href="/search?value1=Yvonne+Pfeifer&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Pfeifer Yvonne</a>, <a href="/search?value1=Martin+A+Fischer&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Fischer Martin A</a>, <a href="/search?value1=Jessica+Eisfeld&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Eisfeld Jessica</a>, <a href="/search?value1=Jennifer+Schauer&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Schauer Jennifer</a>, <a href="/search?value1=Sebastian+Haller&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Haller Sebastian</a>, <a href="/search?value1=Tim+Eckmanns&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Eckmanns Tim</a>, <a href="/search?value1=S%C3%B6ren+Gatermann&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Gatermann Sören</a>, <a href="/search?value1=Guido+Werner&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Werner Guido</a></span>. Molecular surveillance reveals the emergence and dissemination of NDM-5-producing Escherichia coli high-risk clones in Germany, 2013 to 2019. <a href="/content/ecdc">Euro Surveill.</a> 2023;28(10):pii=2200509. <a href="https://doi.org/10.2807/1560-7917.ES.2023.28.10.2200509" title="doi link" target="_blank">https://doi.org/10.2807/1560-7917.ES.2023.28.10.2200509</a> <span class="ES_Article_citation"> <span class="generated">Received</span>: 17 Jun 2022;&nbsp;&nbsp; <span class="generated">Accepted</span>: 17 Jan 2023 </span> </p>
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