1887
  1. Home
  2. Eurosurveillance
  3. Volume 26, Issue 37, 16/Sep/2021
  4. Article
Rapid communication Open Access
Like 0
Download

Abstract

We describe two false-negative results in the detection of meticillin-resistant (MRSA) of sequence type 398 and type t011 using the Cepheid Xpert MRSA NxG assay. The isolates were recovered in late February and early March 2021 from two patients in different hospitals in the northern Netherlands. Variations between the two isolate genomes indicate that this MRSA strain might have been spreading for some time and could have disseminated to other regions of the Netherlands and other European countries.

© Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Loading

Article metrics loading...

/content/10.2807/1560-7917.ES.2021.26.37.2100800
2021-09-16
2024-12-21
/content/10.2807/1560-7917.ES.2021.26.37.2100800
Loading
Loading full text...

Full text loading...

/deliver/fulltext/eurosurveillance/26/37/eurosurv-26-37-2.html?itemId=/content/10.2807/1560-7917.ES.2021.26.37.2100800&mimeType=html&fmt=ahah

References

  1. The European Committee on Antimicrobial Susceptibility Testing (EUCAST). Breakpoint tables for interpretation of MICs and zone diameters. Version 11.0. Växjö: EUCAST; 2021. Available from: http://www.eucast.org
  2. Tavakol M, Riekerink RG, Sampimon OC, van Wamel WJ, van Belkum A, Lam TJ. Bovine-associated MRSA ST398 in the Netherlands. Acta Vet Scand. 2012;54(1):28.  https://doi.org/10.1186/1751-0147-54-28  PMID: 22549050 
  3. Bosch T, de Neeling AJ, Schouls LM, Zwaluw KW, Kluytmans JA, Grundmann H, et al. PFGE diversity within the methicillin-resistant Staphylococcus aureus clonal lineage ST398. BMC Microbiol. 2010;10(1):40.  https://doi.org/10.1186/1471-2180-10-40  PMID: 20144202 
  4. Gómez-Sanz E, Kadlec K, Feßler AT, Zarazaga M, Torres C, Schwarz S. Novel erm(T)-carrying multiresistance plasmids from porcine and human isolates of methicillin-resistant Staphylococcus aureus ST398 that also harbor cadmium and copper resistance determinants. Antimicrob Agents Chemother. 2013;57(7):3275-82.  https://doi.org/10.1128/AAC.00171-13  PMID: 23629701 
  5. Bosch T, Witteveen S, Haenen A, Landman F, Schouls LM. Next-generation sequencing confirms presumed nosocomial transmission of livestock-associated methicillin-resistant Staphylococcus aureus in the Netherlands. Appl Environ Microbiol. 2016;82(14):4081-9.  https://doi.org/10.1128/AEM.00773-16  PMID: 27129960 
  6. Wang L, Safo M, Archer GL. Characterization of DNA sequences required for the CcrAB-mediated integration of staphylococcal cassette chromosome mec, a Staphylococcus aureus genomic island. J Bacteriol. 2012;194(2):486-98.  https://doi.org/10.1128/JB.05047-11  PMID: 22056931 
  7. Lakhundi S, Zhang K. Methicillin-resistant Staphylococcus aureus: molecular characterization, evolution, and epidemiology. Clin Microbiol Rev. 2018;31(4):e00020-18.  https://doi.org/10.1128/CMR.00020-18  PMID: 30209034 
  8. McClure JA, Conly JM, Obasuyi O, Ward L, Ugarte-Torres A, Louie T, et al. A novel assay for detection of methicillin-resistant Staphylococcus aureus directly from clinical samples. Front Microbiol. 2020;11:1295.  https://doi.org/10.3389/fmicb.2020.01295  PMID: 32625187 
  9. Yarbrough ML, Warren DK, Allen K, Burkholder D, Daum R, Donskey C, et al. Multicenter evaluation of the Xpert MRSA NxG assay for detection of methicillin-resistant Staphylococcus aureus in nasal swabs. J Clin Microbiol. 2018;56(1):e01381-17.  https://doi.org/10.1128/JCM.01381-17  PMID: 29118165 
  10. Monecke S, König E, Earls MR, Leitner E, Müller E, Wagner GE, et al. An epidemic CC1-MRSA-IV clone yields false-negative test results in molecular MRSA identification assays: a note of caution, Austria, Germany, Ireland, 2020. Euro Surveill. 2020;25(25):2000929.  https://doi.org/10.2807/1560-7917.ES.2020.25.25.2000929  PMID: 32613938 
  11. Tenover FC, Tickler IA, Le VM, Dewell S, Mendes RE, Goering RV. Updating molecular diagnostics for detecting methicillin-susceptible and methicillin-resistant Staphylococcus aureus isolates in blood culture bottles. J Clin Microbiol. 2019;57(11):e01195-19.  https://doi.org/10.1128/JCM.01195-19  PMID: 31484703 
Misidentification of meticillin-resistant Staphylococcus aureus by the Cepheid Xpert MRSA NxG assay, the Netherlands, February to March 2021
<p class="citation"> <span>Citation style for this article:</span> <span class="meta-value authors"> <a href="/search?value1=Artur+J+Sabat&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Sabat Artur J</a>, <a href="/search?value1=Erik+Bathoorn&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Bathoorn Erik</a>, <a href="/search?value1=Monika+A+Chlebowicz-Fliss&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Chlebowicz-Fliss Monika A</a>, <a href="/search?value1=Viktoria+Akkerboom&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Akkerboom Viktoria</a>, <a href="/search?value1=Inge+Kamphuis&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Kamphuis Inge</a>, <a href="/search?value1=Claudy+Oliveira+dos+Santos&option1=author&noRedirect=true" class="nonDisambigAuthorLink">dos Santos Claudy Oliveira</a>, <a href="/search?value1=Alexander+W+Friedrich&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Friedrich Alexander W</a></span>. Misidentification of meticillin-resistant Staphylococcus aureus by the Cepheid Xpert MRSA NxG assay, the Netherlands, February to March 2021. <a href="/content/ecdc">Euro Surveill.</a> 2021;26(37):pii=2100800. <a href="https://doi.org/10.2807/1560-7917.ES.2021.26.37.2100800" title="doi link" target="_blank">https://doi.org/10.2807/1560-7917.ES.2021.26.37.2100800</a> <span class="ES_Article_citation"> <span class="generated">Received</span>: 13 Aug 2021;&nbsp;&nbsp; <span class="generated">Accepted</span>: 15 Sept 2021 </span> </p>
/content/10.2807/1560-7917.ES.2021.26.37.2100800
/content/10.2807/1560-7917.ES.2021.26.37.2100800
Loading

Data & Media loading...

Submit comment
Close
Comment moderation successfully completed
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error