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Abstract

Two ceftazidime-avibactam (CAZ-AVI)-resistant carbapenemase (KPC)-positive strains, including one pandrug resistant, were isolated in 2019 from two Greek hospitals. The strains were sequence types (ST)s 258 and 147 and both harboured similar self-transmissible IncA/C2 plasmids encoding a novel Lys234Arg variant of the Vietnamese extended-spectrum β-lactamase (VEB)-1, not inhibited by AVI (VEB-25). Conjugal transfer of VEB-25-encoding plasmids to yielded CAZ-AVI-resistant clones, supporting that VEB-25 is directly linked to the derived phenotype.

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/content/10.2807/1560-7917.ES.2020.25.2.1900766
2020-01-16
2024-12-26
/content/10.2807/1560-7917.ES.2020.25.2.1900766
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References

  1. Spiliopoulou I, Kazmierczak K, Stone GG. In vitro activity of ceftazidime/avibactam against isolates of carbapenem-non-susceptible Enterobacteriaceae collected during the INFORM global surveillance programme (2015-17). J Antimicrob Chemother. 2019;dkz456.  https://doi.org/10.1093/jac/dkz456  PMID: 31742604 
  2. Shields RK, Chen L, Cheng S, Chavda KD, Press EG, Snyder A, et al. Emergence of ceftazidime-avibactam resistance due to plasmid-borne blaKPC-3 mutations during treatment of carbapenem-resistant Klebsiella pneumoniae infections. Antimicrob Agents Chemother. 2017;61(3):e02097-16.  https://doi.org/10.1128/AAC.02097-16  PMID: 28031201 
  3. Haidar G, Clancy CJ, Shields RK, Hao B, Cheng S, Nguyen MH. Mutations in blaKPC-3 that confer ceftazidime-avibactam resistance encode novel KPC-3 variants that function as extended-spectrum β-lactamases. Antimicrob Agents Chemother. 2017;61(5):e02534-16.  https://doi.org/10.1128/AAC.02534-16  PMID: 28223379 
  4. Gaibani P, Campoli C, Lewis RE, Volpe SL, Scaltriti E, Giannella M, et al. In vivo evolution of resistant subpopulations of KPC-producing Klebsiella pneumoniae during ceftazidime/avibactam treatment. J Antimicrob Chemother. 2018;73(6):1525-9.  https://doi.org/10.1093/jac/dky082  PMID: 29566151 
  5. Räisänen K, Koivula I, Ilmavirta H, Puranen S, Kallonen T, Lyytikäinen O, et al. Emergence of ceftazidime-avibactam-resistant Klebsiella pneumoniae during treatment, Finland, December 2018. Euro Surveill. 2019;24(19):1900256.  https://doi.org/10.2807/1560-7917.ES.2019.24.19.1900256  PMID: 31088601 
  6. European Centre for Disease Prevention and Control (ECDC). Emergence of resistance to ceftazidime-avibactam in carbapenem-resistant Enterobacteriaceae - 12 June 2018. Stockholm; ECDC; 2018. Available from: https://ecdc.europa. eu/en/publications-data/rapid-risk-assessment-emergence-resistance-ceftazidime-avibactam-carbapenem
  7. The European Committee on Antimicrobial Susceptibility Testing (EUCAST). Breakpoint tables for interpretation of MICs and zone diameters. Version 9.0. Växjö: EUCAST; 2019. Available from; http://www.eucast.org.
  8. Poirel L, Naas T, Guibert M, Chaibi EB, Labia R, Nordmann P. Molecular and biochemical characterization of VEB-1, a novel class A extended-spectrum β-lactamase encoded by an Escherichia coli integron gene. Antimicrob Agents Chemother. 1999;43(3):573-81.  https://doi.org/10.1128/AAC.43.3.573  PMID: 10049269 
  9. Prinarakis EE, Miriagou V, Tzelepi E, Gazouli M, Tzouvelekis LS. Emergence of an inhibitor-resistant β-lactamase (SHV-10) derived from an SHV-5 variant. Antimicrob Agents Chemother. 1997;41(4):838-40.  https://doi.org/10.1128/AAC.41.4.838  PMID: 9087500 
  10. Mendonça N, Manageiro V, Robin F, Salgado MJ, Ferreira E, Caniça M, et al. The Lys234Arg substitution in the enzyme SHV-72 is a determinant for resistance to clavulanic acid inhibition. Antimicrob Agents Chemother. 2008;52(5):1806-11.  https://doi.org/10.1128/AAC.01381-07  PMID: 18316518 
  11. Dubois V, Poirel L, Demarthe F, Arpin C, Coulange L, Minarini LA, et al. Molecular and biochemical characterization of SHV-56, a novel inhibitor-resistant β-lactamase from Klebsiella pneumoniae. Antimicrob Agents Chemother. 2008;52(10):3792-4.  https://doi.org/10.1128/AAC.00387-08  PMID: 18663019 
  12. Manageiro V, Ferreira E, Albuquerque L, Bonnet R, Caniça M. Biochemical study of a new inhibitor-resistant β-lactamase, SHV-84, produced by a clinical Escherichia coli strain. Antimicrob Agents Chemother. 2010;54(5):2271-2.  https://doi.org/10.1128/AAC.01442-09  PMID: 20211886 
  13. Winkler ML, Papp-Wallace KM, Taracila MA, Bonomo RA. Avibactam and inhibitor-resistant SHV β-lactamases. Antimicrob Agents Chemother. 2015;59(7):3700-9.  https://doi.org/10.1128/AAC.04405-14  PMID: 25691639 
  14. Papp-Wallace KM, Winkler ML, Taracila MA, Bonomo RA. Variants of β-lactamase KPC-2 that are resistant to inhibition by avibactam. Antimicrob Agents Chemother. 2015;59(7):3710-7.  https://doi.org/10.1128/AAC.04406-14  PMID: 25666153 
  15. Poirel L, Villa L, Bertini A, Pitout JD, Nordmann P, Carattoli A. Expanded-spectrum β-lactamase and plasmid-mediated quinolone resistance. Emerg Infect Dis. 2007;13(5):803-5.  https://doi.org/10.3201/eid1305.061293  PMID: 18044054 
  16. Papagiannitsis CC, Tryfinopoulou K, Giakkoupi P, Pappa O, Polemis M, Tzelepi E, et al. , Carbapenemase Study Group. Diversity of acquired β-lactamases amongst Klebsiella pneumoniae in Greek hospitals. Int J Antimicrob Agents. 2012;39(2):178-80.  https://doi.org/10.1016/j.ijantimicag.2011.09.024  PMID: 22104281 
  17. Giakkoupi P, Tryfinopoulou K, Polemis M, Pappa O, Miriagou V, Vatopoulos A. Circulation of a multiresistant, conjugative, IncA/C plasmid within the nosocomial Providencia stuartii population in the Athens area. Diagn Microbiol Infect Dis. 2015;82(1):62-4.  https://doi.org/10.1016/j.diagmicrobio.2015.02.009  PMID: 25752202 
Detection in two hospitals of transferable ceftazidime-avibactam resistance in Klebsiella pneumoniae due to a novel VEB β-lactamase variant with a Lys234Arg substitution, Greece, 2019
<p class="citation"> <span>Citation style for this article:</span> <span class="meta-value authors"> <a href="/search?value1=E.+Voulgari&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Voulgari E.</a>, <a href="/search?value1=S.D.+Kotsakis&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Kotsakis S.D.</a>, <a href="/search?value1=P.+Giannopoulou&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Giannopoulou P.</a>, <a href="/search?value1=E.+Perivolioti&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Perivolioti E.</a>, <a href="/search?value1=L.S.+Tzouvelekis&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Tzouvelekis L.S.</a>, <a href="/search?value1=V.+Miriagou&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Miriagou V.</a></span>. Detection in two hospitals of transferable ceftazidime-avibactam resistance in Klebsiella pneumoniae due to a novel VEB β-lactamase variant with a Lys234Arg substitution, Greece, 2019. <a href="/content/ecdc">Euro Surveill.</a> 2020;25(2):pii=1900766. <a href="https://doi.org/10.2807/1560-7917.ES.2020.25.2.1900766" title="doi link" target="_blank">https://doi.org/10.2807/1560-7917.ES.2020.25.2.1900766</a> <span class="ES_Article_citation"> <span class="generated">Received</span>: 27 Dec 2019;&nbsp;&nbsp; <span class="generated">Accepted</span>: 16 Jan 2020 </span> </p>
/content/10.2807/1560-7917.ES.2020.25.2.1900766
/content/10.2807/1560-7917.ES.2020.25.2.1900766
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