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Abstract

Background

Carbapenem-resistant Enterobacteriaceae pose a serious threat to public health worldwide, and the role of companion animals as a reservoir is still unclear.

Aims

This 4-month prospective observational study evaluated carriage of carbapenem-resistant Enterobacteriaceae at admission and after hospitalisation in a large referral hospital for companion animals in Switzerland.

Methods

Rectal swabs of dogs and cats expected to be hospitalised for at least 48 h were taken from May to August 2018 and analysed for the presence of carbapenem-resistant Enterobacteriaceae using selective agar plates. Resistant isolates were further characterised analysing whole genome sequences for resistance gene and plasmid identification, and ad hoc core genome multilocus sequence typing.

Results

This study revealed nosocomial acquisition of harbouring the carbapenemase gene , the pAmpC cephalosporinase gene as well as quinolone resistance associated with and mutations in the topoisomerases II (GyrA) and IV (ParC). The and genes were identified on a 51 kb IncX3 plasmid and on a 47 kb IncI1 plasmid. All isolates belonged to sequence type ST410 and were genetically highly related. This clone was detected in 17 of 100 dogs and four of 34 cats after hospitalisation (21.6%), only one of the tested animals having tested positive at admission (0.75%). Two positive animals were still carriers 4 months after hospital discharge, but were negative after 6 months.

Conclusions

Companion animals may acquire carbapenemase-producing during hospitalisation, posing the risk of further dissemination to the animal and human population and to the environment.

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This work is licensed under a Creative Commons Attribution 4.0 International License.
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/content/10.2807/1560-7917.ES.2019.24.39.1900071
2019-09-26
2024-11-22
http://instance.metastore.ingenta.com/content/10.2807/1560-7917.ES.2019.24.39.1900071
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Shedding of OXA-181 carbapenemase-producing Escherichia coli from companion animals after hospitalisation in Switzerland: an outbreak in 2018
<p class="citation"> <span>Citation style for this article:</span> <span class="meta-value authors"> <a href="/search?value1=Aur%C3%A9lien+Nigg&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Nigg Aurélien</a>, <a href="/search?value1=Michael+Brilhante&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Brilhante Michael</a>, <a href="/search?value1=Valentina+Dazio&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Dazio Valentina</a>, <a href="/search?value1=Mathieu+Cl%C3%A9ment&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Clément Mathieu</a>, <a href="/search?value1=Alexandra+Collaud&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Collaud Alexandra</a>, <a href="/search?value1=Stefanie+Gobeli+Brawand&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Gobeli Brawand Stefanie</a>, <a href="/search?value1=Barbara+Willi&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Willi Barbara</a>, <a href="/search?value1=Andrea+Endimiani&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Endimiani Andrea</a>, <a href="/search?value1=Simone+Schuller&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Schuller Simone</a>, <a href="/search?value1=Vincent+Perreten&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Perreten Vincent</a></span>. Shedding of OXA-181 carbapenemase-producing Escherichia coli from companion animals after hospitalisation in Switzerland: an outbreak in 2018. <a href="/content/ecdc">Euro Surveill.</a> 2019;24(39):pii=1900071. <a href="https://doi.org/10.2807/1560-7917.ES.2019.24.39.1900071" title="doi link" target="_blank">https://doi.org/10.2807/1560-7917.ES.2019.24.39.1900071</a> <span class="ES_Article_citation"> <span class="generated">Received</span>: 22 Jan 2019;&nbsp;&nbsp; <span class="generated">Accepted</span>: 19 May 2019 </span> </p>
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