Highly heterogeneous temperature sensitivity of 2009 pandemic influenza A(H1N1) viral isolates, northern France

I Pelletier; D Rousset; V Enouf; GROG; F Colbère-Garapin; S van der Werf; N Naffakh

doi:10.2807/ese.16.43.19999-en

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Highly heterogeneous temperature sensitivity of 2009 pandemic influenza A(H1N1) viral isolates, northern France
I Pelletier^1,2 , D Rousset^3,4,5,6 , V Enouf^3,4,5,6 , GROG⁷ , F Colbère-Garapin^1,2 , S van der Werf^3,4,5,6 , N Naffakh^3,4,5
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Affiliations: ¹ Institut Pasteur, Unité de Biologie des Virus Entériques, Département de Virologie, Paris, France ² INSERM U994 (French National Institute of Health and Medical Research) Paris, France ³ Institut Pasteur, Unité de Génétique Moléculaire des virus à ARN, Département de Virologie, Paris, France ⁴ CNRS URA3015 (French National Centre for Scientific Research), Paris, France ⁵ Université Paris Diderot, Sorbonne Paris Cité, Unité de Génétique Moléculaire des virus à ARN, Paris, France ⁶ Institut Pasteur, Centre National de Référence des virus influenzae (Région Nord), Paris, France ⁷ Groupes Régionaux d'Observation de la Grippe, Open Rome, Paris, France

Correspondence:
N Naffakh
nadia.naffakh pasteur.fr
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Citation style for this article: Pelletier I, Rousset D, Enouf V, GROG, Colbère-Garapin F, van der Werf S, Naffakh N. Highly heterogeneous temperature sensitivity of 2009 pandemic influenza A(H1N1) viral isolates, northern France. Euro Surveill. 2011;16(43):pii=19999. https://doi.org/10.2807/ese.16.43.19999-en Received: 19 Jul 2011

Abstract

We assayed the temperature sensitivity of 2009 pandemic influenza A(H1N1) viral isolates (n=23) and seasonal influenza A(H1N1) viruses (n=18) isolated in northern France in 2007/08 and 2008/09. All isolates replicated with a similar efficiency at 34 °C and 37 °C, and with a lower efficiency at 40 °C. The pandemic viral isolates showed a stronger heterogeneity in their ability to grow at the highest temperature, as compared with the seasonal isolates. No statistically significant difference in temperature sensitivity was observed between the pandemic viral isolates from severe and mild cases of influenza. Our data point to the impact of temperature sensitivity on the genetic evolution and diversification of the pandemic influenza A(H1N1) virus since its introduction into the human population in April 2009, and call for close surveillance of this phenotypic marker related to host and tissue tropism. .