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SDG 3: Antimicrobial resistance

Abstract

Background

Antimicrobial resistance (AMR) is a global threat. Monitoring using an integrated One Health approach is essential to detect changes in AMR occurrence.

Aim

We aimed to detect AMR genes in pathogenic and commensal collected 2013–2020 within monitoring programmes and research from food animals, food (fresh retail raw meat) and humans in six European countries, to compare vertical and horizontal transmission.

Methods

We whole genome sequenced (WGS) 3,745 isolates detected AMR genes using ResFinder and performed phylogenetic analysis to determine isolate relatedness and transmission. A BLASTn-based bioinformatic method compared draft IncI1 genomes to conserved plasmid references from Europe.

Results

Resistance genes to medically important antimicrobials (MIA) such as extended-spectrum cephalosporins (ESC) were widespread but predicted resistance to MIAs authorised for human use (carbapenem, tigecycline) was detected only in two human and three cattle isolates. Phylogenetic analysis clustered according to phylogroups; commensal animal isolates showed greater diversity than those from human patients. Only 18 vertical animal-food and human-animal transmission events of clones were detected. However, IncI1 plasmids from different sources and/or countries carrying resistance to ESCs were conserved and widely distributed, although these variants were rarely detected in human pathogens.

Conclusion

Using WGS we demonstrated AMR is driven vertically and horizontally. Human clinical isolates were more closely related, but their IncI1 plasmids were more diverse, while animal or food isolates were less similar with more conserved IncI1 plasmids. These differences likely arose from variations in selective pressure, influencing AMR evolution and transmission.

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This work is licensed under a Creative Commons Attribution 4.0 International License.
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2024-11-21
2024-11-22
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Escherichia coli from six European countries reveals differences in profile and distribution of critical antimicrobial resistance determinants within One Health compartments, 2013 to 2020
<p class="citation"> <span>Citation style for this article:</span> <span class="meta-value authors"> <a href="/search?value1=H%C3%A5kon+P+Kaspersen&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Kaspersen Håkon P</a>, <a href="/search?value1=Michael+SM+Brouwer&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Brouwer Michael SM</a>, <a href="/search?value1=Javier+Nunez-Garcia&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Nunez-Garcia Javier</a>, <a href="/search?value1=Ingrid+C%C3%A1rdenas-Rey&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Cárdenas-Rey Ingrid</a>, <a href="/search?value1=Manal+AbuOun&option1=author&noRedirect=true" class="nonDisambigAuthorLink">AbuOun Manal</a>, <a href="/search?value1=Nicholas+Duggett&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Duggett Nicholas</a>, <a href="/search?value1=Nicholas+Ellaby&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Ellaby Nicholas</a>, <a href="/search?value1=Jose+Delgado-Blas&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Delgado-Blas Jose</a>, <a href="/search?value1=Jens+A+Hammerl&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Hammerl Jens A</a>, <a href="/search?value1=Maria+Getino&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Getino Maria</a>, <a href="/search?value1=Carlos+Serna&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Serna Carlos</a>, <a href="/search?value1=Thierry+Naas&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Naas Thierry</a>, <a href="/search?value1=Kees+T+Veldman&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Veldman Kees T</a>, <a href="/search?value1=Alex+Bossers&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Bossers Alex</a>, <a href="/search?value1=Marianne+Sunde&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Sunde Marianne</a>, <a href="/search?value1=Solveig+S+Mo&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Mo Solveig S</a>, <a href="/search?value1=Silje+B+J%C3%B8rgensen&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Jørgensen Silje B</a>, <a href="/search?value1=Matthew+Ellington&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Ellington Matthew</a>, <a href="/search?value1=Bruno+Gonzalez-Zorn&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Gonzalez-Zorn Bruno</a>, <a href="/search?value1=Roberto+La+Ragione&option1=author&noRedirect=true" class="nonDisambigAuthorLink">La Ragione Roberto</a>, <a href="/search?value1=Philippe+Glaser&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Glaser Philippe</a>, <a href="/search?value1=Muna+F+Anjum&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Anjum Muna F</a></span>. Escherichia coli from six European countries reveals differences in profile and distribution of critical antimicrobial resistance determinants within One Health compartments, 2013 to 2020. <a href="/content/ecdc">Euro Surveill.</a> 2024;29(47):pii=2400295. <a href="https://doi.org/10.2807/1560-7917.ES.2024.29.47.2400295" title="doi link" target="_blank">https://doi.org/10.2807/1560-7917.ES.2024.29.47.2400295</a> <span class="ES_Article_citation"> <span class="generated">Received</span>: 14 May 2024;&nbsp;&nbsp; <span class="generated">Accepted</span>: 07 Oct 2024 </span> </p>
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