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Abstract

Background

The number of cholera cases reported to the World Health Organization (WHO) in 2022 was more than double that of 2021. Nine countries of the WHO European Region reported 51 cases of cholera in 2022 vs five reported cases in 2021.

Aim

We aimed to confirm that the O1 isolates reported by WHO European Region countries in 2022 belonged to the seventh pandemic El Tor lineage (7PET). We also studied their virulence, antimicrobial resistance (AMR) determinants and phylogenetic relationships.

Methods

We used microbial genomics to study the 49 O1 isolates recovered from the 51 European cases. We also used > 1,450 publicly available 7PET genomes to provide a global phylogenetic context for these 49 isolates.

Results

All 46 good-quality genomes obtained belonged to the 7PET lineage. All but two isolates belonged to genomic Wave 3 and were grouped within three sub-lineages, one of which, Pre-AFR15, predominated (34/44). This sub-lineage, corresponding to isolates from several countries in Southern Asia, the Middle East and Eastern or Southern Africa, was probably a major contributor to the global upsurge of cholera cases in 2022. No unusual AMR profiles were inferred from analysis of the AMR gene content of the 46 genomes.

Conclusion

Reference laboratories in high-income countries should use whole genome sequencing to assign O1 isolates formally to the 7PET or non-epidemic lineages. Periodic collaborative genomic studies based on isolates from travellers can provide useful information on the circulating strains and their evolution, particularly as concerns AMR.

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This work is licensed under a Creative Commons Attribution 4.0 International License.
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2024-09-05
2024-09-07
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Genomic analysis of Vibrio cholerae O1 isolates from cholera cases, Europe, 2022
<p class="citation"> <span>Citation style for this article:</span> <span class="meta-value authors"> <a href="/search?value1=Caroline+Rouard&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Rouard Caroline</a>, <a href="/search?value1=David+R+Greig&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Greig David R</a>, <a href="/search?value1=Thamida+Tauhid&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Tauhid Thamida</a>, <a href="/search?value1=Susann+Dupke&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Dupke Susann</a>, <a href="/search?value1=Elisabeth+Njamkepo&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Njamkepo Elisabeth</a>, <a href="/search?value1=Ettore+Amato&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Amato Ettore</a>, <a href="/search?value1=Boas+van+der+Putten&option1=author&noRedirect=true" class="nonDisambigAuthorLink">van der Putten Boas</a>, <a href="/search?value1=Umaer+Naseer&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Naseer Umaer</a>, <a href="/search?value1=Marion+Blaschitz&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Blaschitz Marion</a>, <a href="/search?value1=Georgia+D+Mandilara&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Mandilara Georgia D</a>, <a href="/search?value1=James++Cohen+Stuart&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Cohen Stuart James </a>, <a href="/search?value1=Alexander+Indra&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Indra Alexander</a>, <a href="/search?value1=Harold+No%C3%ABl&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Noël Harold</a>, <a href="/search?value1=Theologia+Sideroglou&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Sideroglou Theologia</a>, <a href="/search?value1=Florian+Heger&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Heger Florian</a>, <a href="/search?value1=Maaike+van+den+Beld&option1=author&noRedirect=true" class="nonDisambigAuthorLink">van den Beld Maaike</a>, <a href="/search?value1=Astrid+Louise+Wester&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Wester Astrid Louise</a>, <a href="/search?value1=Marie-Laure+Quilici&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Quilici Marie-Laure</a>, <a href="/search?value1=Holger+C+Scholz&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Scholz Holger C</a>, <a href="/search?value1=Inga+Fr%C3%B6ding&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Fröding Inga</a>, <a href="/search?value1=Claire+Jenkins&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Jenkins Claire</a>, <a href="/search?value1=Fran%C3%A7ois-Xavier+Weill&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Weill François-Xavier</a></span>. Genomic analysis of Vibrio cholerae O1 isolates from cholera cases, Europe, 2022. <a href="/content/ecdc">Euro Surveill.</a> 2024;29(36):pii=2400069. <a href="https://doi.org/10.2807/1560-7917.ES.2024.29.36.2400069" title="doi link" target="_blank">https://doi.org/10.2807/1560-7917.ES.2024.29.36.2400069</a> <span class="ES_Article_citation"> <span class="generated">Received</span>: 31 Jan 2024;&nbsp;&nbsp; <span class="generated">Accepted</span>: 06 May 2024 </span> </p>
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