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Abstract

Anal and urethral samples from confirmed cases of monkeypox were screened for monkeypox virus (MPXV) by real-time PCR. Isolation of the virus was subsequently attempted in cell culture. Actively-replicating virus was demonstrated in 13 of 18 and 11 of 15 PCR-positive anal and urethral swabs, respectively, collected within 7 days from symptoms onset. Two asymptomatic secondary cases had detectable MPXV genetic material in urethral secretion and for one, MPXV was successfully isolated, supporting a potential MPXV sexual transmission hypothesis.

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/content/10.2807/1560-7917.ES.2022.27.36.2200675
2022-09-08
2024-11-21
http://instance.metastore.ingenta.com/content/10.2807/1560-7917.ES.2022.27.36.2200675
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Isolation of viable monkeypox virus from anal and urethral swabs, Italy, May to July 2022
<p class="citation"> <span>Citation style for this article:</span> <span class="meta-value authors"> <a href="/search?value1=Davide+Moschese&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Moschese Davide</a>, <a href="/search?value1=Giacomo+Pozza&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Pozza Giacomo</a>, <a href="/search?value1=Davide+Mileto&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Mileto Davide</a>, <a href="/search?value1=Andrea+Giacomelli&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Giacomelli Andrea</a>, <a href="/search?value1=Miriam+Cutrera&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Cutrera Miriam</a>, <a href="/search?value1=Maria+Vittoria+Cossu&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Cossu Maria Vittoria</a>, <a href="/search?value1=Maddalena+Matone&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Matone Maddalena</a>, <a href="/search?value1=Martina+Beltrami&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Beltrami Martina</a>, <a href="/search?value1=Federica+Salari&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Salari Federica</a>, <a href="/search?value1=Spinello+Antinori&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Antinori Spinello</a>, <a href="/search?value1=Alessandra+Lombardi&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Lombardi Alessandra</a>, <a href="/search?value1=Giuliano+Rizzardini&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Rizzardini Giuliano</a></span>. Isolation of viable monkeypox virus from anal and urethral swabs, Italy, May to July 2022. <a href="/content/ecdc">Euro Surveill.</a> 2022;27(36):pii=2200675. <a href="https://doi.org/10.2807/1560-7917.ES.2022.27.36.2200675" title="doi link" target="_blank">https://doi.org/10.2807/1560-7917.ES.2022.27.36.2200675</a> <span class="ES_Article_citation"> <span class="generated">Received</span>: 23 Aug 2022;&nbsp;&nbsp; <span class="generated">Accepted</span>: 08 Sept 2022 </span> </p>
/content/10.2807/1560-7917.ES.2022.27.36.2200675
/content/10.2807/1560-7917.ES.2022.27.36.2200675
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