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Abstract

Introduction

Numerous CE-marked SARS-CoV-2 antigen rapid diagnostic tests (Ag RDT) are offered in Europe, several of them with unconfirmed quality claims.

Aim

We performed an independent head-to-head evaluation of the sensitivity of SARS-CoV-2 Ag RDT offered in Germany.

Methods

We addressed the sensitivity of 122 Ag RDT in direct comparison using a common evaluation panel comprised of 50 specimens. Minimum sensitivity of 75% for panel specimens with a PCR quantification cycle (Cq) ≤ 25 was used to identify Ag RDT eligible for reimbursement in the German healthcare system.

Results

The sensitivity of different SARS-CoV-2 Ag RDT varied over a wide range. The sensitivity limit of 75% for panel members with Cq ≤ 25 was met by 96 of the 122 tests evaluated; 26 tests exhibited lower sensitivity, few of which failed completely. Some RDT exhibited high sensitivity, e.g. 97.5 % for Cq < 30.

Conclusions

This comparative evaluation succeeded in distinguishing less sensitive from better performing Ag RDT. Most of the evaluated Ag RDT appeared to be suitable for fast identification of acute infections associated with high viral loads. Market access of SARS-CoV-2 Ag RDT should be based on minimal requirements for sensitivity and specificity.

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A corrected article has been published for this content:
Author's correction for Euro Surveill. 2021;26(44)
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2021-11-04
2024-12-24
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Comparative sensitivity evaluation for 122 CE-marked rapid diagnostic tests for SARS-CoV-2 antigen, Germany, September 2020 to April 2021
<p class="citation"> <span>Citation style for this article:</span> <span class="meta-value authors"> <a href="/search?value1=Heinrich+Scheiblauer&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Scheiblauer Heinrich</a>, <a href="/search?value1=Angela+Filomena&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Filomena Angela</a>, <a href="/search?value1=Andreas+Nitsche&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Nitsche Andreas</a>, <a href="/search?value1=Andreas+Puyskens&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Puyskens Andreas</a>, <a href="/search?value1=Victor+M+Corman&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Corman Victor M</a>, <a href="/search?value1=Christian+Drosten&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Drosten Christian</a>, <a href="/search?value1=Karin+Zwirglmaier&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Zwirglmaier Karin</a>, <a href="/search?value1=Constanze+Lange&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Lange Constanze</a>, <a href="/search?value1=Petra+Emmerich&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Emmerich Petra</a>, <a href="/search?value1=Michael+M%C3%BCller&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Müller Michael</a>, <a href="/search?value1=Olivia+Knauer&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Knauer Olivia</a>, <a href="/search?value1=C+Micha+N%C3%BCbling&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Nübling C Micha</a></span>. Comparative sensitivity evaluation for 122 CE-marked rapid diagnostic tests for SARS-CoV-2 antigen, Germany, September 2020 to April 2021. <a href="/content/ecdc">Euro Surveill.</a> 2021;26(44):pii=2100441. <a href="https://doi.org/10.2807/1560-7917.ES.2021.26.44.2100441" title="doi link" target="_blank">https://doi.org/10.2807/1560-7917.ES.2021.26.44.2100441</a> <span class="ES_Article_citation"> <span class="generated">Received</span>: 07 May 2021;&nbsp;&nbsp; <span class="generated">Accepted</span>: 14 Oct 2021 </span> </p>
/content/10.2807/1560-7917.ES.2021.26.44.2100441
/content/10.2807/1560-7917.ES.2021.26.44.2100441
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