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Abstract

Introduction

Standard testing for infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is based on RT-PCR tests, but detection of viral genetic material alone does not indicate ongoing infectious potential. The ability to isolate whole virus represents a better proxy for infectivity.

Aim

The objective of this study was to gain an understanding of the current literature and compare the reported periods of positive SARS-CoV-2 detection from studies that conducted RT-PCR testing in addition to experiments isolating whole virus.

Methods

Using a rapid review approach, studies reporting empirical data on the duration of positive RT-PCR results and/or successful viral isolation following SARS-CoV-2 infection in humans were identified through searches of peer-reviewed and pre-print health sciences literature. Articles were screened for relevance, then data were extracted, analysed, and synthesised.

Results

Of the 160 studies included for qualitative analysis, 84% (n = 135) investigated duration of positive RT-PCR tests only, 5% (n = 8) investigated duration of successful viral isolations, while 11% (n = 17) included measurements on both. There was significant heterogeneity in reported data. There was a prolonged time to viral clearance when deduced from RT-PCR tests compared with viral isolations (median: 26 vs 9 days).

Discussion

Findings from this review support a minimum 10-day period of isolation but certain cases where virus was isolated after 10 days were identified. Given the extended time to viral clearance from RT-PCR tests, future research should ensure standard reporting of RT-PCR protocols and results to help inform testing policies aimed at clearance from isolation.

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This work is licensed under a Creative Commons Attribution 4.0 International License.
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2021-04-08
2024-12-03
http://instance.metastore.ingenta.com/content/10.2807/1560-7917.ES.2021.26.14.2001506
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Determining the communicable period of SARS-CoV-2: A rapid review of the literature, March to September 2020
<p class="citation"> <span>Citation style for this article:</span> <span class="meta-value authors"> <a href="/search?value1=Mina+Park&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Park Mina</a>, <a href="/search?value1=Colleen+Pawliuk&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Pawliuk Colleen</a>, <a href="/search?value1=Tribesty+Nguyen&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Nguyen Tribesty</a>, <a href="/search?value1=Amanda+Griffitt&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Griffitt Amanda</a>, <a href="/search?value1=Linda+Dix-Cooper&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Dix-Cooper Linda</a>, <a href="/search?value1=Nadia+Fourik&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Fourik Nadia</a>, <a href="/search?value1=Martin+Dawes&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Dawes Martin</a></span>. Determining the communicable period of SARS-CoV-2: A rapid review of the literature, March to September 2020. <a href="/content/ecdc">Euro Surveill.</a> 2021;26(14):pii=2001506. <a href="https://doi.org/10.2807/1560-7917.ES.2021.26.14.2001506" title="doi link" target="_blank">https://doi.org/10.2807/1560-7917.ES.2021.26.14.2001506</a> <span class="ES_Article_citation"> <span class="generated">Received</span>: 05 Aug 2020;&nbsp;&nbsp; <span class="generated">Accepted</span>: 31 Dec 2020 </span> </p>
/content/10.2807/1560-7917.ES.2021.26.14.2001506
/content/10.2807/1560-7917.ES.2021.26.14.2001506
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