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Prior infection by seasonal coronaviruses, as assessed by serology, does not prevent SARS-CoV-2 infection and disease in children, France, April to June 2020
- Isabelle Sermet-Gaudelus1,2,3,4 , Sarah Temmam4,5 , Christèle Huon5 , Sylvie Behillil6,7 , Vincent Gajdos8,9 , Thomas Bigot5,10 , Thibaut Lurier11,12,13 , Delphine Chrétien5 , Marija Backovic14 , Agnès Delaunay-Moisan15 , Flora Donati6,7 , Mélanie Albert6,7 , Elsa Foucaud16 , Bettina Mesplées17 , Grégoire Benoist18 , Albert Faye19 , Marc Duval-Arnould20 , Célia Cretolle2 , Marina Charbit2 , Mélodie Aubart2 , Johanne Auriau2 , Mathie Lorrot21 , Dulanjalee Kariyawasam2 , Laura Fertitta2 , Gilles Orliaguet2 , Bénédicte Pigneur2 , Brigitte Bader-Meunier2 , Coralie Briand17 , Vincent Enouf6,7,22 , Julie Toubiana2,3,23 , Tiffany Guilleminot24 , Sylvie van der Werf6,7 , Marianne Leruez-Ville24 , Marc Eloit5,25
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View Affiliations Hide AffiliationsAffiliations: 1 Institut Necker Enfants Malades, INSERM U 1171, Paris, France 2 Hôpital Necker-Enfants Malades, Assistance Publique Hôpitaux de Paris, Paris, France 3 Université de Paris, Paris, France 4 These authors contributed equally to the work 5 Pathogen Discovery Laboratory, Department of Virology, Institut Pasteur, Paris, France 6 Molecular Genetics of RNA Viruses, Department of Virology, CNRS UMR3569, Université de Paris, Institut Pasteur, Paris, France 7 National Reference Center for Respiratory Viruses, Institut Pasteur, Paris, France 8 Hôpital Antoine Beclere, Clamart, France 9 Centre for Research in Epidemiology and Population Health, INSERM UMR1018, Villejuif, France 10 Hub de Bioinformatique et Biostatistique – Département Biologie Computationnelle, Institut Pasteur, USR 3756 CNRS, Paris, France 11 Université Clermont Auvergne, INRAE, VetAgro Sup, UMR EPIA, Saint-Genès-Champanelle, France 12 Université de Lyon, INRAE, VetAgro Sup, UMR EPIA, Marcy l’Etoile, France 13 Université de Lyon, INRAE, VetAgro Sup, Usc 1233 UR RS2GP, Marcy l’Etoile, France 14 Unité de Virologie Structurale, Institut Pasteur, Département de Virologie, CNRS, UMR3569, Paris, France 15 Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), Gif-sur-Yvette, France 16 Hôpital Jean Verdier, Bondy, France 17 Hôpital Louis Mourier, Colombes, France 18 Hôpital Ambroise Paré, Boulogne Billancourt, France 19 Hôpital Robert Debré, Paris, France 20 Hôpital Kremlin Bicêtre, Le Kremlin-Bicêtre, France 21 Hôpital Armand Trousseau, Paris, France 22 Plateforme de microbiologie mutualisée (P2M), Pasteur International Bioresources Network (PIBnet), Institut Pasteur, Paris, France 23 Unité Biodiversité et Epidemiologie des Bacteries Pathogènes, Institut Pasteur, Paris, France 24 Laboratoire de Microbiologie, Hôpital Necker-Enfants Malades, Paris, France 25 Ecole Nationale Vétérinaire d’Alfort, Maisons Alfort, FranceMarc Eloitmarc.eloit pasteur.fr
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Citation style for this article: Sermet-Gaudelus Isabelle, Temmam Sarah, Huon Christèle, Behillil Sylvie, Gajdos Vincent, Bigot Thomas, Lurier Thibaut, Chrétien Delphine, Backovic Marija, Delaunay-Moisan Agnès, Donati Flora, Albert Mélanie, Foucaud Elsa, Mesplées Bettina, Benoist Grégoire, Faye Albert, Duval-Arnould Marc, Cretolle Célia, Charbit Marina, Aubart Mélodie, Auriau Johanne, Lorrot Mathie, Kariyawasam Dulanjalee, Fertitta Laura, Orliaguet Gilles, Pigneur Bénédicte, Bader-Meunier Brigitte, Briand Coralie, Enouf Vincent, Toubiana Julie, Guilleminot Tiffany, van der Werf Sylvie, Leruez-Ville Marianne, Eloit Marc. Prior infection by seasonal coronaviruses, as assessed by serology, does not prevent SARS-CoV-2 infection and disease in children, France, April to June 2020. Euro Surveill. 2021;26(13):pii=2001782. https://doi.org/10.2807/1560-7917.ES.2021.26.13.2001782 Received: 09 Oct 2020; Accepted: 07 Mar 2021
Abstract
Children have a low rate of COVID-19 and secondary severe multisystem inflammatory syndrome (MIS) but present a high prevalence of symptomatic seasonal coronavirus infections.
We tested if prior infections by seasonal coronaviruses (HCoV) NL63, HKU1, 229E or OC43 as assessed by serology, provide cross-protective immunity against SARS-CoV-2 infection.
We set a cross-sectional observational multicentric study in pauci- or asymptomatic children hospitalised in Paris during the first wave for reasons other than COVID (hospitalised children (HOS), n = 739) plus children presenting with MIS (n = 36). SARS-CoV-2 antibodies directed against the nucleoprotein (N) and S1 and S2 domains of the spike (S) proteins were monitored by an in-house luciferase immunoprecipitation system assay. We randomly selected 69 SARS-CoV-2-seropositive patients (including 15 with MIS) and 115 matched SARS-CoV-2-seronegative patients (controls (CTL)). We measured antibodies against SARS-CoV-2 and HCoV as evidence for prior corresponding infections and assessed if SARS-CoV-2 prevalence of infection and levels of antibody responses were shaped by prior seasonal coronavirus infections.
Prevalence of HCoV infections were similar in HOS, MIS and CTL groups. Antibody levels against HCoV were not significantly different in the three groups and were not related to the level of SARS-CoV-2 antibodies in the HOS and MIS groups. SARS-CoV-2 antibody profiles were different between HOS and MIS children.
Prior infection by seasonal coronaviruses, as assessed by serology, does not interfere with SARS-CoV-2 infection and related MIS in children.
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