1887
  1. Home
  2. Eurosurveillance
  3. Volume 25, Issue 5, 06/Feb/2020
  4. Article
Rapid communication Open Access
Like 0
Download

Abstract

The Finnish new variant of (FI-nvCT) is escaping diagnostics in Finland, Norway and Sweden. We have developed and validated an Aptima-format nucleic acid amplification test (NAAT) designed specifically to detect the FI-nvCT. This NAAT has high sensitivity (100%) and specificity (100%) for the FI-nvCT strain, enabling further investigation of the geographic distribution, prevalence and transmission of this diagnostic-escape mutant in screening populations in Europe.

© Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Loading

Article metrics loading...

/content/10.2807/1560-7917.ES.2020.25.5.2000038
2020-02-06
2024-12-22
/content/10.2807/1560-7917.ES.2020.25.5.2000038
Loading
Loading full text...

Full text loading...

/deliver/fulltext/eurosurveillance/25/5/eurosurv-25-5-4.html?itemId=/content/10.2807/1560-7917.ES.2020.25.5.2000038&mimeType=html&fmt=ahah

References

  1. Rantakokko-Jalava K, Hokynar K, Hieta N, Keskitalo A, Jokela P, Muotiala A, et al. Chlamydia trachomatis samples testing falsely negative in the Aptima Combo 2 test in Finland, 2019. Euro Surveill. 2019;24(22):1900298.  https://doi.org/10.2807/1560-7917.ES.2019.24.22.1900298  PMID: 31164192 
  2. Unemo M, Getman D, Hadad R, Cole M, Thomson N, Puolakkainen M, et al. Letter to the editor: Chlamydia trachomatis samples testing falsely negative in the Aptima Combo 2 test in Finland, 2019. Euro Surveill. 2019;24(24):1900354.  https://doi.org/10.2807/1560-7917.ES.2019.24.24.1900354  PMID: 31213219 
  3. Hokynar K, Rantakokko-Jalava K, Hakanen A, Havana M, Mannonen L, Jokela P, et al. The Finnish new variant of Chlamydia trachomatis with a single nucleotide polymorphism in the 23S rRNA target escapes detection by the Aptima Combo 2 test. Microorganisms. 2019;7(8):E227.  https://doi.org/10.3390/microorganisms7080227  PMID: 31370214 
  4. Unemo M, Hansen M, Hadad R, Lindroth Y, Fredlund H, Puolakkainen M, et al. Finnish new variant of Chlamydia trachomatis escaping detection in the Aptima Combo 2 assay also present in Örebro County, Sweden, May 2019. Euro Surveill. 2019;24(26):1900370.  https://doi.org/10.2807/1560-7917.ES.2019.24.26.1900370  PMID: 31266590 
  5. Johansen TB, Kløvstad H, Rykkvin R, Herrfurth-Erichsen EB, Sorthe J, Njølstad G, et al. The ‘Finnish new variant of Chlamydia trachomatis’ escaping detection in the Aptima Combo 2 assay is widespread across Norway, June to August 2019. Euro Surveill. 2019;24(42):1900592.  https://doi.org/10.2807/1560-7917.ES.2019.24.42.1900592  PMID: 31640843 
  6. National Committee for Clinical Laboratory Standards (NCCLS). EP17-A: Protocols for Determination of Limits of Detection and Limits of Quantitation; Approved Guideline. Wayne, Pennsylvania: NCCLS; 2004.
  7. Rowley J, Vander Hoorn S, Korenromp E, Low N, Unemo M, Abu-Raddad LJ, et al. Chlamydia, gonorrhoea, trichomoniasis and syphilis: global prevalence and incidence estimates, 2016. Bull World Health Organ. 2019;97(8):548-562P.  https://doi.org/10.2471/BLT.18.228486  PMID: 31384073 
  8. Hadfield J, Harris SR, Seth-Smith HMB, Parmar S, Andersson P, Giffard PM, et al. Comprehensive global genome dynamics of Chlamydia trachomatis show ancient diversification followed by contemporary mixing and recent lineage expansion. Genome Res. 2017;27(7):1220-9.  https://doi.org/10.1101/gr.212647.116  PMID: 28588068 
  9. Ripa T, Nilsson P. A variant of Chlamydia trachomatis with deletion in cryptic plasmid: implications for use of PCR diagnostic tests. Euro Surveill. 2006;11(11):E061109.2. PMID: 17213548 
  10. Herrmann B, Törner A, Low N, Klint M, Nilsson A, Velicko I, et al. Emergence and spread of Chlamydia trachomatis variant, Sweden. Emerg Infect Dis. 2008;14(9):1462-5.  https://doi.org/10.3201/eid1409.080153  PMID: 18760021 
  11. Unemo M, Clarke IN. The Swedish new variant of Chlamydia trachomatis. Curr Opin Infect Dis. 2011;24(1):62-9.  https://doi.org/10.1097/QCO.0b013e32834204d5  PMID: 21157332 
  12. Jurstrand M, Fredlund H, Unemo M. The new variant of Chlamydia trachomatis was present as early as 2003 in Orebro County, Sweden, but remained undetected until 2006. Sex Transm Infect. 2013;89(7):607-8.  https://doi.org/10.1136/sextrans-2013-051018  PMID: 23580610 
Validation of an Aptima-format Finnish new variant of Chlamydia trachomatis (FI-nvCT) surveillance assay, 2019
<p class="citation"> <span>Citation style for this article:</span> <span class="meta-value authors"> <a href="/search?value1=Barbara+Weinbaum&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Weinbaum Barbara</a>, <a href="/search?value1=Analee+Williams&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Williams Analee</a>, <a href="/search?value1=Ronza+Hadad&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Hadad Ronza</a>, <a href="/search?value1=Bryan+Vinluan&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Vinluan Bryan</a>, <a href="/search?value1=Mirja+Puolakkainen&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Puolakkainen Mirja</a>, <a href="/search?value1=Magnus+Unemo&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Unemo Magnus</a>, <a href="/search?value1=Damon+Getman&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Getman Damon</a></span>. Validation of an Aptima-format Finnish new variant of Chlamydia trachomatis (FI-nvCT) surveillance assay, 2019. <a href="/content/ecdc">Euro Surveill.</a> 2020;25(5):pii=2000038. <a href="https://doi.org/10.2807/1560-7917.ES.2020.25.5.2000038" title="doi link" target="_blank">https://doi.org/10.2807/1560-7917.ES.2020.25.5.2000038</a> <span class="ES_Article_citation"> <span class="generated">Received</span>: 16 Jan 2020;&nbsp;&nbsp; <span class="generated">Accepted</span>: 05 Feb 2020 </span> </p>
/content/10.2807/1560-7917.ES.2020.25.5.2000038
/content/10.2807/1560-7917.ES.2020.25.5.2000038
Loading

Data & Media loading...

Submit comment
Close
Comment moderation successfully completed
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error