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Abstract

Background

Emerging antimicrobial resistance (AMR) challenges gonorrhoea treatment and requires surveillance.

Aim

This observational study describes the genetic diversity of isolates in Germany from 2014 to 2017 and identifies multi-antigen sequence typing (NG-MAST) genogroups associated with AMR or some patient demographics.

Methods

1,220 gonococcal isolates underwent AMR testing and NG-MAST. Associations between genogroups and AMR or sex/age of patients were statistically assessed.

Results

Patients’ median age was 32 years (interquartile range: 25–44); 1,078 isolates (88.4%) originated from men. In total, 432 NG-MAST sequence types including 156 novel ones were identified, resulting in 17 major genogroups covering 59.1% (721/1,220) of all isolates. Genogroups G1407 and G10557 (G7072) were significantly associated with decreased susceptibility to cefixime (Kruskal–Wallis chi-squared: 549.3442, df: 16, p < 0.001). Their prevalences appeared to decline during the study period from 14.2% (15/106) to 6.2% (30/481) and from 6.6% (7/106) to 3.1% (15/481) respectively. Meanwhile, several cefixime susceptible genogroups’ prevalence seemed to increase. Proportions of isolates from men differed among genogroups (Fisher’s exact test, p < 0.001), being e.g. lower for G25 (G51) and G387, and higher for G5441 and G2992. Some genogroups differed relative to each other in affected patients’ median age (Kruskal–Wallis chi-squared:  47.5358, df:  16, p < 0.001), with e.g. G25 (G51) and G387 more frequent among ≤ 30 year olds and G359 and G17420 among ≥ 40 year olds.

Conclusion

AMR monitoring with molecular typing is important. Dual therapy (ceftriaxone plus azithromycin) recommended in 2014 in Germany, or only the ceftriaxone dose of this therapy, might have contributed to cefixime-resistant genogroups decreasing.

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2020-10-15
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Molecular epidemiological typing of Neisseria gonorrhoeae isolates identifies a novel association between genogroup G10557 (G7072) and decreased susceptibility to cefixime, Germany, 2014 to 2017
<p class="citation"> <span>Citation style for this article:</span> <span class="meta-value authors"> <a href="/search?value1=Sebastian+Banhart&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Banhart Sebastian</a>, <a href="/search?value1=Klaus+Jansen&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Jansen Klaus</a>, <a href="/search?value1=Susanne+Buder&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Buder Susanne</a>, <a href="/search?value1=Thalea+Tamminga&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Tamminga Thalea</a>, <a href="/search?value1=S%C3%A9bastien+Calvignac-Spencer&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Calvignac-Spencer Sébastien</a>, <a href="/search?value1=Tanja+Pilz&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Pilz Tanja</a>, <a href="/search?value1=Andrea+Martini&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Martini Andrea</a>, <a href="/search?value1=Sandra+Dudareva&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Dudareva Sandra</a>, <a href="/search?value1=Sergejs+Nikisins&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Nikisins Sergejs</a>, <a href="/search?value1=Kerstin+Dehmel&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Dehmel Kerstin</a>, <a href="/search?value1=Gabriele+Zuelsdorf&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Zuelsdorf Gabriele</a>, <a href="/search?value1=Eva+Guhl&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Guhl Eva</a>, <a href="/search?value1=Ingeborg+Graeber&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Graeber Ingeborg</a>, <a href="/search?value1=Peter+K+Kohl&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Kohl Peter K</a>, <a href="/search?value1=Magnus+Unemo&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Unemo Magnus</a>, <a href="/search?value1=Viviane+Bremer&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Bremer Viviane</a>, <a href="/search?value1=Dagmar+Heuer&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Heuer Dagmar</a>, <a href="/search?value1=on+behalf+of+the+GORENET+study+group&option1=author&noRedirect=true" class="nonDisambigAuthorLink">on behalf of the GORENET study group</a></span>. Molecular epidemiological typing of Neisseria gonorrhoeae isolates identifies a novel association between genogroup G10557 (G7072) and decreased susceptibility to cefixime, Germany, 2014 to 2017. <a href="/content/ecdc">Euro Surveill.</a> 2020;25(41):pii=1900648. <a href="https://doi.org/10.2807/1560-7917.ES.2020.25.41.1900648" title="doi link" target="_blank">https://doi.org/10.2807/1560-7917.ES.2020.25.41.1900648</a> <span class="ES_Article_citation"> <span class="generated">Received</span>: 24 Oct 2019;&nbsp;&nbsp; <span class="generated">Accepted</span>: 10 Aug 2020 </span> </p>
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