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Abstract

We found that a single nucleotide polymorphism (SNP) in the nucleoprotein gene of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) from a patient interfered with detection in a widely used commercial assay. Some 0.2% of the isolates in the EpiCoV database contain this SNP. Although SARS-CoV-2 was still detected by the other probe in the assay, this underlines the necessity of targeting two independent essential regions of a pathogen for reliable detection.

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/content/10.2807/1560-7917.ES.2020.25.39.2001650
2020-10-01
2024-11-23
http://instance.metastore.ingenta.com/content/10.2807/1560-7917.ES.2020.25.39.2001650
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SARS-CoV-2 samples may escape detection because of a single point mutation in the N gene
<p class="citation"> <span>Citation style for this article:</span> <span class="meta-value authors"> <a href="/search?value1=Katharina+Ziegler&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Ziegler Katharina</a>, <a href="/search?value1=Philipp+Steininger&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Steininger Philipp</a>, <a href="/search?value1=Renate+Ziegler&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Ziegler Renate</a>, <a href="/search?value1=J%C3%B6rg+Steinmann&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Steinmann Jörg</a>, <a href="/search?value1=Klaus+Korn&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Korn Klaus</a>, <a href="/search?value1=Armin+Ensser&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Ensser Armin</a></span>. SARS-CoV-2 samples may escape detection because of a single point mutation in the N gene. <a href="/content/ecdc">Euro Surveill.</a> 2020;25(39):pii=2001650. <a href="https://doi.org/10.2807/1560-7917.ES.2020.25.39.2001650" title="doi link" target="_blank">https://doi.org/10.2807/1560-7917.ES.2020.25.39.2001650</a> <span class="ES_Article_citation"> <span class="generated">Received</span>: 10 Sept 2020;&nbsp;&nbsp; <span class="generated">Accepted</span>: 30 Sept 2020 </span> </p>
/content/10.2807/1560-7917.ES.2020.25.39.2001650
/content/10.2807/1560-7917.ES.2020.25.39.2001650
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