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Abstract

Background

A novel coronavirus, SARS-CoV-2, which emerged at the end of 2019 and causes COVID-19, has resulted in worldwide human infections. While genetically distinct, SARS-CoV-1, the aetiological agent responsible for an outbreak of severe acute respiratory syndrome (SARS) in 2002–2003, utilises the same host cell receptor as SARS-CoV-2 for entry: angiotensin-converting enzyme 2 (ACE2). Parts of the SARS-CoV-1 spike glycoprotein (S protein), which interacts with ACE2, appear conserved in SARS-CoV-2.

Aim

The cross-reactivity with SARS-CoV-2 of monoclonal antibodies (mAbs) previously generated against the S protein of SARS-CoV-1 was assessed.

Methods

The SARS-CoV-2 S protein sequence was aligned to those of SARS-CoV-1, Middle East respiratory syndrome (MERS) and common-cold coronaviruses. Abilities of mAbs generated against SARS-CoV-1 S protein to bind SARS-CoV-2 or its S protein were tested with SARS-CoV-2 infected cells as well as cells expressing either the full length protein or a fragment of its S2 subunit. Quantitative ELISA was also performed to compare binding of mAbs to recombinant S protein.

Results

An immunogenic domain in the S2 subunit of SARS-CoV-1 S protein is highly conserved in SARS-CoV-2 but not in MERS and human common-cold coronaviruses. Four murine mAbs raised against this immunogenic fragment could recognise SARS-CoV-2 S protein expressed in mammalian cell lines. In particular, mAb 1A9 was demonstrated to detect S protein in SARS-CoV-2-infected cells and is suitable for use in a sandwich ELISA format.

Conclusion

The cross-reactive mAbs may serve as useful tools for SARS-CoV-2 research and for the development of diagnostic assays for COVID-19.

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This work is licensed under a Creative Commons Attribution 4.0 International License.
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2020-07-16
2024-11-21
http://instance.metastore.ingenta.com/content/10.2807/1560-7917.ES.2020.25.28.2000291
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Monoclonal antibodies for the S2 subunit of spike of SARS-CoV-1 cross-react with the newly-emerged SARS-CoV-2
<p class="citation"> <span>Citation style for this article:</span> <span class="meta-value authors"> <a href="/search?value1=Zhiqiang+Zheng&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Zheng Zhiqiang</a>, <a href="/search?value1=Vanessa+Marthe+Monteil&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Monteil Vanessa Marthe</a>, <a href="/search?value1=Sebastian+Maurer-Stroh&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Maurer-Stroh Sebastian</a>, <a href="/search?value1=Chow+Wenn+Yew&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Yew Chow Wenn</a>, <a href="/search?value1=Carol+Leong&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Leong Carol</a>, <a href="/search?value1=Nur+Khairiah+Mohd-Ismail&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Mohd-Ismail Nur Khairiah</a>, <a href="/search?value1=Suganya++Cheyyatraivendran+Arularasu&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Cheyyatraivendran Arularasu Suganya </a>, <a href="/search?value1=Vincent+Tak+Kwong+Chow&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Chow Vincent Tak Kwong</a>, <a href="/search?value1=Raymond+Tzer+Pin+Lin&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Lin Raymond Tzer Pin</a>, <a href="/search?value1=Ali+Mirazimi&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Mirazimi Ali</a>, <a href="/search?value1=Wanjin+Hong&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Hong Wanjin</a>, <a href="/search?value1=Yee-Joo+Tan&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Tan Yee-Joo</a></span>. Monoclonal antibodies for the S2 subunit of spike of SARS-CoV-1 cross-react with the newly-emerged SARS-CoV-2. <a href="/content/ecdc">Euro Surveill.</a> 2020;25(28):pii=2000291. <a href="https://doi.org/10.2807/1560-7917.ES.2020.25.28.2000291" title="doi link" target="_blank">https://doi.org/10.2807/1560-7917.ES.2020.25.28.2000291</a> <span class="ES_Article_citation"> <span class="generated">Received</span>: 12 Mar 2020;&nbsp;&nbsp; <span class="generated">Accepted</span>: 11 Jun 2020 </span> </p>
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