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Abstract

Containment strategies and clinical management of coronavirus disease (COVID-19) patients during the current pandemic depend on reliable diagnostic PCR assays for the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Here, we compare 11 different RT-PCR test systems used in seven diagnostic laboratories in Germany in March 2020. While most assays performed well, we identified detection problems in a commonly used assay that may have resulted in false-negative test results during the first weeks of the pandemic.

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/content/10.2807/1560-7917.ES.2020.25.24.2001057
2020-06-18
2024-12-22
/content/10.2807/1560-7917.ES.2020.25.24.2001057
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Multicentre comparison of quantitative PCR-based assays to detect SARS-CoV-2, Germany, March 2020
<p class="citation"> <span>Citation style for this article:</span> <span class="meta-value authors"> <a href="/search?value1=Maximilian+Muenchhoff&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Muenchhoff Maximilian</a>, <a href="/search?value1=Helga+Mairhofer&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Mairhofer Helga</a>, <a href="/search?value1=Hans+Nitschko&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Nitschko Hans</a>, <a href="/search?value1=Natascha+Grzimek-Koschewa&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Grzimek-Koschewa Natascha</a>, <a href="/search?value1=Dieter+Hoffmann&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Hoffmann Dieter</a>, <a href="/search?value1=Annemarie+Berger&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Berger Annemarie</a>, <a href="/search?value1=Holger+Rabenau&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Rabenau Holger</a>, <a href="/search?value1=Marek+Widera&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Widera Marek</a>, <a href="/search?value1=Nikolaus+Ackermann&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Ackermann Nikolaus</a>, <a href="/search?value1=Regina+Konrad&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Konrad Regina</a>, <a href="/search?value1=Sabine+Zange&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Zange Sabine</a>, <a href="/search?value1=Alexander+Graf&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Graf Alexander</a>, <a href="/search?value1=Stefan+Krebs&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Krebs Stefan</a>, <a href="/search?value1=Helmut+Blum&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Blum Helmut</a>, <a href="/search?value1=Andreas+Sing&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Sing Andreas</a>, <a href="/search?value1=Bernhard+Liebl&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Liebl Bernhard</a>, <a href="/search?value1=Roman+W%C3%B6lfel&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Wölfel Roman</a>, <a href="/search?value1=Sandra+Ciesek&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Ciesek Sandra</a>, <a href="/search?value1=Christian+Drosten&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Drosten Christian</a>, <a href="/search?value1=Ulrike+Protzer&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Protzer Ulrike</a>, <a href="/search?value1=Stephan+Boehm&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Boehm Stephan</a>, <a href="/search?value1=Oliver+T+Keppler&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Keppler Oliver T</a></span>. Multicentre comparison of quantitative PCR-based assays to detect SARS-CoV-2, Germany, March 2020. <a href="/content/ecdc">Euro Surveill.</a> 2020;25(24):pii=2001057. <a href="https://doi.org/10.2807/1560-7917.ES.2020.25.24.2001057" title="doi link" target="_blank">https://doi.org/10.2807/1560-7917.ES.2020.25.24.2001057</a> <span class="ES_Article_citation"> <span class="generated">Received</span>: 28 May 2020;&nbsp;&nbsp; <span class="generated">Accepted</span>: 17 Jun 2020 </span> </p>
/content/10.2807/1560-7917.ES.2020.25.24.2001057
/content/10.2807/1560-7917.ES.2020.25.24.2001057
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