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Abstract

The World Health Organization has declared COVID-19 caused by the newly discovered SARS-CoV-2 a pandemic. Due to growing demand for reagents and/or kits to extract SARS-CoV-2 RNA for subsequent RT-qPCR diagnostics, there is a worldwide risk of shortages. With a detection sensitivity of 97.4% (95% CI: 86.2–99.9%), we describe a simple, fast, alternative workflow for molecular detection of SARS-CoV-2, where samples are simply heat-processed for 5 min at 98 °C before a commonly-used RT-qPCR procedure.

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/content/10.2807/1560-7917.ES.2020.25.14.2000398
2020-04-09
2024-12-27
/content/10.2807/1560-7917.ES.2020.25.14.2000398
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References

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An alternative workflow for molecular detection of SARS-CoV-2 – escape from the NA extraction kit-shortage, Copenhagen, Denmark, March 2020
<p class="citation"> <span>Citation style for this article:</span> <span class="meta-value authors"> <a href="/search?value1=Anna+S.+Fomsgaard&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Fomsgaard Anna S.</a>, <a href="/search?value1=Maiken+Wors%C3%B8e+Rosenstierne&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Rosenstierne Maiken Worsøe</a></span>. An alternative workflow for molecular detection of SARS-CoV-2 – escape from the NA extraction kit-shortage, Copenhagen, Denmark, March 2020. <a href="/content/ecdc">Euro Surveill.</a> 2020;25(14):pii=2000398. <a href="https://doi.org/10.2807/1560-7917.ES.2020.25.14.2000398" title="doi link" target="_blank">https://doi.org/10.2807/1560-7917.ES.2020.25.14.2000398</a> <span class="ES_Article_citation"> <span class="generated">Received</span>: 23 Mar 2020;&nbsp;&nbsp; <span class="generated">Accepted</span>: 08 Apr 2020 </span> </p>
/content/10.2807/1560-7917.ES.2020.25.14.2000398
/content/10.2807/1560-7917.ES.2020.25.14.2000398
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