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Abstract

Background

Tick-borne encephalitis (TBE) is a potentially severe neurological disease caused by TBE virus (TBEV). In Europe and Asia, TBEV infection has become a growing public health concern and requires fast and specific detection.

Aim

In this observational study, we evaluated a rapid TBE IgM test, ReaScan TBE, for usage in a clinical laboratory setting.

Methods

Patient sera found negative or positive for TBEV by serological and/or molecular methods in diagnostic laboratories of five European countries endemic for TBEV (Estonia, Finland, Slovenia, the Netherlands and Sweden) were used to assess the sensitivity and specificity of the test. The patients’ diagnoses were based on other commercial or quality assured in-house assays, i.e. each laboratory’s conventional routine methods. For specificity analysis, serum samples from patients with infections known to cause problems in serology were employed. These samples tested positive for e.g. Epstein–Barr virus, cytomegalovirus and , or for flaviviruses other than TBEV, i.e. dengue, Japanese encephalitis, West Nile and Zika viruses. Samples from individuals vaccinated against flaviviruses other than TBEV were also included. Altogether, 172 serum samples from patients with acute TBE and 306 TBE IgM negative samples were analysed.

Results

Compared with each laboratory’s conventional methods, the tested assay had similar sensitivity and specificity (99.4% and 97.7%, respectively). Samples containing potentially interfering antibodies did not cause specificity problems.

Conclusion

Regarding diagnosis of acute TBEV infections, ReaScan TBE offers rapid and convenient complementary IgM detection. If used as a stand-alone, it can provide preliminary results in a laboratory or point of care setting.

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This work is licensed under a Creative Commons Attribution 4.0 International License.
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/content/10.2807/1560-7917.ES.2020.25.12.1900427
2020-03-26
2024-12-03
http://instance.metastore.ingenta.com/content/10.2807/1560-7917.ES.2020.25.12.1900427
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Multi-laboratory evaluation of ReaScan TBE IgM rapid test, 2016 to 2017
<p class="citation"> <span>Citation style for this article:</span> <span class="meta-value authors"> <a href="/search?value1=Bo+Albinsson&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Albinsson Bo</a>, <a href="/search?value1=Anu+E.+J%C3%A4%C3%A4skel%C3%A4inen&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Jääskeläinen Anu E.</a>, <a href="/search?value1=Kairi+V%C3%A4rv&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Värv Kairi</a>, <a href="/search?value1=Mateja+Jelov%C5%A1ek&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Jelovšek Mateja</a>, <a href="/search?value1=Corine+GeurtsvanKessel&option1=author&noRedirect=true" class="nonDisambigAuthorLink">GeurtsvanKessel Corine</a>, <a href="/search?value1=Sirkka+Vene&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Vene Sirkka</a>, <a href="/search?value1=Josef+D.+J%C3%A4rhult&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Järhult Josef D.</a>, <a href="/search?value1=Chantal+Reusken&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Reusken Chantal</a>, <a href="/search?value1=Irina+Golovljova&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Golovljova Irina</a>, <a href="/search?value1=Tatjana+Av%C5%A1i%C4%8D-%C5%BDupanc&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Avšič-Županc Tatjana</a>, <a href="/search?value1=Olli+Vapalahti&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Vapalahti Olli</a>, <a href="/search?value1=%C3%85ke+Lundkvist&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Lundkvist Åke</a></span>. Multi-laboratory evaluation of ReaScan TBE IgM rapid test, 2016 to 2017. <a href="/content/ecdc">Euro Surveill.</a> 2020;25(12):pii=1900427. <a href="https://doi.org/10.2807/1560-7917.ES.2020.25.12.1900427" title="doi link" target="_blank">https://doi.org/10.2807/1560-7917.ES.2020.25.12.1900427</a> <span class="ES_Article_citation"> <span class="generated">Received</span>: 28 Jun 2019;&nbsp;&nbsp; <span class="generated">Accepted</span>: 14 Feb 2020 </span> </p>
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