1887
  1. Home
  2. Eurosurveillance
  3. Volume 24, Issue 38, 19/Sep/2019
  4. Article
Rapid communication Open Access
Like 0
Download

Abstract

We identified two new (CT) variants escaping Aptima Combo 2 (AC2) assay detection, in clinical specimens of two patients. One had a C1514T mutation the other a G1523A mutation, both within the AC2 23S rRNA target region. The prevalence of such variants among persons tested for CT in England was estimated to be fewer than 0.003%.

© Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Addendum
An addendum has been published for this content:
Addendum for Euro Surveill. 2019;24(38)
Loading

Article metrics loading...

/content/10.2807/1560-7917.ES.2019.24.38.1900557
2019-09-19
2024-12-22
/content/10.2807/1560-7917.ES.2019.24.38.1900557
Loading
Loading full text...

Full text loading...

/deliver/fulltext/eurosurveillance/24/38/eurosurv-24-38-1.html?itemId=/content/10.2807/1560-7917.ES.2019.24.38.1900557&mimeType=html&fmt=ahah

References

  1. Rantakokko-Jalava K, Hokynar K, Hieta N, Keskitalo A, Jokela P, Muotiala A, et al. Chlamydia trachomatis samples testing falsely negative in the Aptima Combo 2 test in Finland, 2019. Euro Surveill. 2019;24(22):1900298.  https://doi.org/10.2807/1560-7917.ES.2019.24.22.1900298  PMID: 31164192 
  2. Unemo M, Getman D, Hadad R, Cole M, Thomson N, Puolakkainen M, et al. Letter to the editor: Chlamydia trachomatis samples testing falsely negative in the Aptima Combo 2 test in Finland, 2019. Euro Surveill. 2019;24(24):1900354.  https://doi.org/10.2807/1560-7917.ES.2019.24.24.1900354  PMID: 31213219 
  3. Hologic Inc. Urgent Field Safety Notice (FSN). Aptima Combo 2 Assay (for European Distribution). Brussels: Hologic, Inc.; 2019. Available from: https://mhra.filecamp.com/public/file/3ql3-occ8agbc
  4. European Centre for Disease Prevention and Control (ECDC). Rapid risk assessment: Chlamydia trachomatis false-negative test results by Aptima Combo 2 CT/NG assay (Hologic) in the EU/EEA, 2019. Stockholm: ECDC; 2019. Available from: https://ecdc.europa.eu/en/publications-data/rapid-risk-assessment-chlamydia-trachomatis-false-negative-test-results-aptima
  5. Public Health England (PHE). CTAD Chlamydia Surveillance System. London: PHE; 2012. Available from: https://www.gov.uk/guidance/ctad-chlamydia-surveillance-system
  6. Public Health England (PHE). Sexually transmitted infections (STIs): annual data tables. London: PHE; 2019. Available from: https://www.gov.uk/government/statistics/sexually-transmitted-infections-stis-annual-data-tables
  7. Gaydos CA, Cartwright CP, Colaninno P, Welsch J, Holden J, Ho SY, et al. Performance of the Abbott RealTime CT/NG for detection of Chlamydia trachomatis and Neisseria gonorrhoeae. J Clin Microbiol. 2010;48(9):3236-43.  https://doi.org/10.1128/JCM.01019-10  PMID: 20668135 
  8. Unemo M, Hansen M, Hadad R, Lindroth Y, Fredlund H, Puolakkainen M, et al. Finnish new variant of Chlamydia trachomatis escaping detection in the Aptima Combo 2 assay also present in Örebro County, Sweden, May 2019. Euro Surveill. 2019;24(26):1900370.  https://doi.org/10.2807/1560-7917.ES.2019.24.26.1900370  PMID: 31266590 
  9. Ripa T, Nilsson P. A variant of Chlamydia trachomatis with deletion in cryptic plasmid: implications for use of PCR diagnostic tests. Euro Surveill. 2006;11(11):E061109.2. PMID: 17213548 
  10. Herrmann B, Törner A, Low N, Klint M, Nilsson A, Velicko I, et al. Emergence and spread of Chlamydia trachomatis variant, Sweden. Emerg Infect Dis. 2008;14(9):1462-5.  https://doi.org/10.3201/eid1409.080153  PMID: 18760021 
  11. Sweeney EL, Bletchly C, Gupta R, Whiley DM. False-negative Chlamydia polymerase chain reaction result caused by a cryptic plasmid-deficient Chlamydia trachomatis strain in Australia. Sex Health. 2019;16(4):394-6.  https://doi.org/10.1071/SH18205  PMID: 31270010 
  12. Peterson EM, Markoff BA, Schachter J, de la Maza LM. The 7.5-kb plasmid present in Chlamydia trachomatis is not essential for the growth of this microorganism. Plasmid. 1990;23(2):144-8.  https://doi.org/10.1016/0147-619X(90)90033-9  PMID: 2362949 
  13. Söderblom T, Blaxhult A, Fredlund H, Herrmann B. Impact of a genetic variant of Chlamydia trachomatis on national detection rates in Sweden. Euro Surveill. 2006;11(12):E061207.1. PMID: 17213562 
  14. Savage EJ, Ison CA, van de Laar MJWESSTI network. Results of a Europe-wide investigation to assess the presence of a new variant of Chlamydia trachomatis. Euro Surveill. 2007;12(10):E3-4.  https://doi.org/10.2807/esm.12.10.00736-en  PMID: 17997927 
  15. de Barbeyrac B, Raherison S, Cado S, Normandin F, Clerc M, Clairet V, et al. French situation concerning the Swedish Chlamydia trachomatis variant. Euro Surveill. 2007;12(10):E11-2.  https://doi.org/10.2807/esm.12.10.00740-en  PMID: 17997924 
  16. Morré SA, Catsburg A, de Boer M, Spaargaren J, de Vries HJ, Schirm J, et al. Monitoring the potential introduction of the Swedish Chlamydia trachomatis variant (swCT) in the Netherlands. Euro Surveill. 2007;12(10):E9-10.  https://doi.org/10.2807/esm.12.10.00739-en  PMID: 17997930 
Prevalence of new variants of Chlamydia trachomatis escaping detection by the Aptima Combo 2 assay, England, June to August 2019
<p class="citation"> <span>Citation style for this article:</span> <span class="meta-value authors"> <a href="/search?value1=David+J+Roberts&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Roberts David J</a>, <a href="/search?value1=Grahame+S+Davis&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Davis Grahame S</a>, <a href="/search?value1=Michelle+J+Cole&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Cole Michelle J</a>, <a href="/search?value1=Dixita+Naik&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Naik Dixita</a>, <a href="/search?value1=Hitiksha+Maru&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Maru Hitiksha</a>, <a href="/search?value1=Neil+Woodford&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Woodford Neil</a>, <a href="/search?value1=Peter+Muir&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Muir Peter</a>, <a href="/search?value1=Paddy+Horner&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Horner Paddy</a>, <a href="/search?value1=Ian+Simms&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Simms Ian</a>, <a href="/search?value1=George+Thickett&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Thickett George</a>, <a href="/search?value1=Paul+Crook&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Crook Paul</a>, <a href="/search?value1=Kirsty+Foster&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Foster Kirsty</a>, <a href="/search?value1=Nick+Andrews&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Andrews Nick</a>, <a href="/search?value1=John+Saunders&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Saunders John</a>, <a href="/search?value1=Helen+Fifer&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Fifer Helen</a>, <a href="/search?value1=Kate+Folkard&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Folkard Kate</a>, <a href="/search?value1=O+Noel+Gill&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Gill O Noel</a>, <a href="/search?value1=on+behalf+of+the+incident+management+team&option1=author&noRedirect=true" class="nonDisambigAuthorLink">on behalf of the incident management team</a></span>. Prevalence of new variants of Chlamydia trachomatis escaping detection by the Aptima Combo 2 assay, England, June to August 2019. <a href="/content/ecdc">Euro Surveill.</a> 2019;24(38):pii=1900557. <a href="https://doi.org/10.2807/1560-7917.ES.2019.24.38.1900557" title="doi link" target="_blank">https://doi.org/10.2807/1560-7917.ES.2019.24.38.1900557</a> <span class="ES_Article_citation"> <span class="generated">Received</span>: 05 Sept 2019;&nbsp;&nbsp; <span class="generated">Accepted</span>: 19 Sept 2019 </span> </p>
/content/10.2807/1560-7917.ES.2019.24.38.1900557
/content/10.2807/1560-7917.ES.2019.24.38.1900557
Loading

Data & Media loading...

Submit comment
Close
Comment moderation successfully completed
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error