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Abstract

Introduction

The occurrence of antibiotic resistance in faecal bacteria in sewage is likely to reflect the current local clinical resistance situation.

Aim

This observational study investigated the relationship between resistance rates in sewage and clinical samples representing the same human populations.

Methods

were isolated from eight hospital (n = 721 isolates) and six municipal (n = 531 isolates) sewage samples, over 1 year in Gothenburg, Sweden. An inexpensive broth screening method was validated against disk diffusion and applied to determine resistance against 11 antibiotics in sewage isolates. Resistance data on isolated from clinical samples from corresponding local hospital and primary care patients were collected during the same year and compared with those of the sewage isolates by linear regression.

Results

resistance rates derived from hospital sewage and hospital patients strongly correlated (r2 = 0.95 for urine and 0.89 for blood samples), as did resistance rates in from municipal sewage and primary care urine samples (r2 = 0.82). Resistance rates in hospital sewage isolates were close to those in hospital clinical isolates while resistance rates in municipal sewage isolates were about half of those measured in primary care isolates. Resistance rates in municipal sewage isolates were more stable between sampling occasions than those from hospital sewage.

Conclusion

Our findings provide support for development of a low-cost, sewage-based surveillance system for antibiotic resistance in , which could complement current monitoring systems and provide clinically relevant antibiotic resistance data for countries and regions where surveillance is lacking.

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2019-09-12
2024-11-22
http://instance.metastore.ingenta.com/content/10.2807/1560-7917.ES.2019.24.37.1800497
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Population-level surveillance of antibiotic resistance in Escherichia coli through sewage analysis
<p class="citation"> <span>Citation style for this article:</span> <span class="meta-value authors"> <a href="/search?value1=Marion+Hutinel&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Hutinel Marion</a>, <a href="/search?value1=Patricia+Maria+Catharina+Huijbers&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Huijbers Patricia Maria Catharina</a>, <a href="/search?value1=Jerker+Fick&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Fick Jerker</a>, <a href="/search?value1=Christina+%C3%85hr%C3%A9n&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Åhrén Christina</a>, <a href="/search?value1=Dan+G%C3%B6ran+Joakim+Larsson&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Larsson Dan Göran Joakim</a>, <a href="/search?value1=Carl-Fredrik+Flach&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Flach Carl-Fredrik</a></span>. Population-level surveillance of antibiotic resistance in Escherichia coli through sewage analysis. <a href="/content/ecdc">Euro Surveill.</a> 2019;24(37):pii=1800497. <a href="https://doi.org/10.2807/1560-7917.ES.2019.24.37.1800497" title="doi link" target="_blank">https://doi.org/10.2807/1560-7917.ES.2019.24.37.1800497</a> <span class="ES_Article_citation"> <span class="generated">Received</span>: 10 Sept 2018;&nbsp;&nbsp; <span class="generated">Accepted</span>: 22 May 2019 </span> </p>
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