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Abstract

On 18 January 2016, the French National Reference Centre for reported to Santé publique France an excess of serotype Dublin ( Dublin) infections. We investigated to identify the source of infection and implement control measures. Whole genome sequencing (WGS) and multilocus variable-number tandem repeat analysis (MLVA) were performed to identify microbiological clusters and links among cases, animal and food sources. Clusters were defined as isolates with less than 15 single nucleotide polymorphisms determined by WGS and/or with identical MLVA pattern. We compared different clusters of cases with other cases (case–case study) and controls recruited from a web-based cohort (case–control study) in terms of food consumption. We interviewed 63/83 (76%) cases; 2,914 controls completed a questionnaire. Both studies’ findings indicated that successive Dublin outbreaks from different sources had occurred between November 2015 and March 2016. In the case–control study, cases of distinct WGS clusters were more likely to have consumed Morbier (adjusted odds ratio (aOR): 14; 95% confidence interval (CI): 4.8–42) or Vacherin Mont d’Or (aOR: 27; 95% CI: 6.8–105), two bovine raw-milk cheeses. Based on these results, the Ministry of Agriculture launched a reinforced control plan for processing plants of raw-milk cheeses in the production region, to prevent future outbreaks.

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/content/10.2807/1560-7917.ES.2019.24.3.1700703
2019-01-17
2024-12-21
/content/10.2807/1560-7917.ES.2019.24.3.1700703
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References

  1. Majowicz SE, Musto J, Scallan E, Angulo FJ, Kirk M, O’Brien SJ, et al. , International Collaboration on Enteric Disease ‘Burden of Illness’ Studies. The global burden of nontyphoidal Salmonella gastroenteritis. Clin Infect Dis. 2010;50(6):882-9.  https://doi.org/10.1086/650733  PMID: 20158401 
  2. Langridge GC, Fookes M, Connor TR, Feltwell T, Feasey N, Parsons BN, et al. Patterns of genome evolution that have accompanied host adaptation in Salmonella. Proc Natl Acad Sci USA. 2015;112(3):863-8.  https://doi.org/10.1073/pnas.1416707112  PMID: 25535353 
  3. Grimont PAD, Weill FX. Antigenic formulae of the Salmonella serovars, 9th ed. Paris: World Health Organization Collaborating Center for Reference and Research on Salmonella, Institut Pasteur; 2007. Available from: https://www.pasteur.fr/sites/default/files/veng_0.pdf
  4. Jones TF, Ingram LA, Cieslak PR, Vugia DJ, Tobin-D’Angelo M, Hurd S, et al. Salmonellosis outcomes differ substantially by serotype. J Infect Dis. 2008;198(1):109-14.  https://doi.org/10.1086/588823  PMID: 18462137 
  5. Helms M, Vastrup P, Gerner-Smidt P, Mølbak K. Short and long term mortality associated with foodborne bacterial gastrointestinal infections: registry based study. BMJ. 2003;326(7385):357.  https://doi.org/10.1136/bmj.326.7385.357  PMID: 12586666 
  6. Mohammed M, Cormican M. Whole genome sequencing provides insights into the genetic determinants of invasiveness in Salmonella Dublin. Epidemiol Infect. 2016;144(11):2430-9.  https://doi.org/10.1017/S0950268816000492  PMID: 26996313 
  7. El Sayed F, Sapriel G, Fawal N, Gruber A, Bauer T, Heym B, et al. In-Host Adaptation of Salmonella enterica Serotype Dublin during Prosthetic Hip Joint Infection. Emerg Infect Dis. 2018;24(12):2360-3.  https://doi.org/10.3201/eid2412.180214  PMID: 30457550 
  8. Selander RK, Smith NH, Li J, Beltran P, Ferris KE, Kopecko DJ, et al. Molecular evolutionary genetics of the cattle-adapted serovar Salmonella dublin. J Bacteriol. 1992;174(11):3587-92.  https://doi.org/10.1128/jb.174.11.3587-3592.1992  PMID: 1592813 
  9. Werner SB, Humphrey GL, Kamei I. Association between raw milk and human Salmonella dublin infection. BMJ. 1979;2(6184):238-41.  https://doi.org/10.1136/bmj.2.6184.238  PMID: 476402 
  10. Giron S, Le Hello S, Salah S, Mazur M, Lailler R, Barataud D, et al. Salmonella Dublin: what is its potential severity? Investigating a national foodborne outbreak of Salmonellosis associated with the consumption of raw milk cheese, France, August-September 2012. Poster session presented at: 6th International Symposium Salmonella and Salmonellosis (I3S); 2013 May 27-29; Saint-Malo, France.
  11. Vignaud M-L, Cherchame E, Marault M, Chaing E, Le Hello S, Michel V, et al. MLVA for Salmonella enterica subsp. enterica Serovar Dublin: Development of a Method Suitable for Inter-Laboratory Surveillance and Application in the Context of a Raw Milk Cheese Outbreak in France in 2012. Front Microbiol. 2017;8:295.  https://doi.org/10.3389/fmicb.2017.00295  PMID: 28289408 
  12. David JM, Sanders P, Bemrah N, Granier SA, Denis M, Weill FX, et al. Attribution of the French human Salmonellosis cases to the main food-sources according to the type of surveillance data. Prev Vet Med. 2013;110(1):12-27.  https://doi.org/10.1016/j.prevetmed.2013.02.002  PMID: 23453456 
  13. Jones G, Le Hello S, Jourdan-da Silva N, Vaillant V, de Valk H, Weill F, et al. The French human Salmonella surveillance system: evaluation of timeliness of laboratory reporting and factors associated with delays, 2007 to 2011. Euro Surveill. 2014;19(1):20664.  https://doi.org/10.2807/1560-7917.ES2014.19.1.20664  PMID: 24434174 
  14. Jourdan-Da Silva N, Le Hello S. [Salmonellosis in 2002-2010 in France: trends in human epidemiology, monophasic serotype emergence, main food implicated in the latest outbreaks]. Bulletin Epidémiologique Hebdomadaire Hors-série. 2012;25-33. French.
  15. Kjeldsen MK, Torpdahl M, Campos J, Pedersen K, Nielsen EM. Multiple-locus variable-number tandem repeat analysis of Salmonella enterica subsp. enterica serovar Dublin. J Appl Microbiol. 2014;116(4):1044-54.  https://doi.org/10.1111/jam.12441  PMID: 24517207 
  16. Mohammed M, Delappe N, O’Connor J, McKeown P, Garvey P, Cormican M. Whole genome sequencing provides an unambiguous link between Salmonella Dublin outbreak strain and a historical isolate. Epidemiol Infect. 2016;144(3):576-81.  https://doi.org/10.1017/S0950268815001636  PMID: 26165314 
  17. Ågren EC, Wahlström H, Vesterlund-Carlson C, Lahti E, Melin L, Söderlund R. Comparison of whole genome sequencing typing results and epidemiological contact information from outbreaks of Salmonella Dublin in Swedish cattle herds. Infect Ecol Epidemiol. 2016;6(1):31782.  https://doi.org/10.3402/iee.v6.31782  PMID: 27396609 
  18. Gossner C, Le Hello S, De Jong B, Rolfhamre P, Faensen D, Weill FX, et al. Around the World in 1,475 Salmonella Geo-serotypes. Emerg Infect Dis. 2016;22(7):1298-302.  https://doi.org/10.3201/eid2207.141678 
  19. Fonteneau L, Jourdan Da Silva N, Fabre L, Ashton P, Torpdahl M, Müller L, et al. Multinational outbreak of travel-related Salmonella Chester infections in Europe, summers 2014 and 2015. Euro Surveill. 2017;22(7):30463.  https://doi.org/10.2807/1560-7917.ES.2017.22.7.30463  PMID: 28230522 
  20. Langmead B, Salzberg SL. Fast gapped-read alignment with Bowtie 2. Nat Methods. 2012;9(4):357-9.  https://doi.org/10.1038/nmeth.1923  PMID: 22388286 
  21. Treangen TJ, Ondov BD, Koren S, Phillippy AM. The Harvest suite for rapid core-genome alignment and visualization of thousands of intraspecific microbial genomes. Genome Biol. 2014;15(11):524.  https://doi.org/10.1186/s13059-014-0524-x  PMID: 25410596 
  22. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Mol Biol Evol. 2013;30(12):2725-9.  https://doi.org/10.1093/molbev/mst197  PMID: 24132122 
  23. Letunic I, Bork P. Interactive tree of life (iTOL) v3: an online tool for the display and annotation of phylogenetic and other trees. Nucleic Acids Res. 2016;44(W1):W242-5.  https://doi.org/10.1093/nar/gkw290  PMID: 27095192 
  24. Bachelerie P, Felten A, Vignaud ML, Glasset B, Feurer C, Lailler R, et al. MLVA_Normalizer: Workflow for Normalization of MLVA Profiles and Data Exchange between Laboratories. J Proteomics Bioinform. 2016;9(2):25-7.  https://doi.org/10.4172/jpb.1000385 
  25. Debin M, Turbelin C, Blanchon T, Bonmarin I, Falchi A, Hanslik T, et al. Evaluating the feasibility and participants’ representativeness of an online nationwide surveillance system for influenza in France. PLoS One. 2013;8(9):e73675.  https://doi.org/10.1371/journal.pone.0073675  PMID: 24040020 
  26. Guerrisi C, Turbelin C, Blanchon T, Hanslik T, Bonmarin I, Levy-Bruhl D, et al. Participatory syndromic surveillance of influenza in Europe. J Infect Dis. 2016;214(suppl_4):S386-92.  https://doi.org/10.1093/infdis/jiw280  PMID: 28830105 
  27. Achtman M, Wain J, Weill FX, Nair S, Zhou Z, Sangal V, et al. , S. Enterica MLST Study Group. Multilocus sequence typing as a replacement for serotyping in Salmonella enterica. PLoS Pathog. 2012;8(6):e1002776.  https://doi.org/10.1371/journal.ppat.1002776  PMID: 22737074 
  28. Mook P, Kanagarajah S, Maguire H, Adak GK, Dabrera G, Waldram A, et al. Selection of population controls for a Salmonella case-control study in the UK using a market research panel and web-survey provides time and resource savings. Epidemiol Infect. 2016;144(6):1220-30.  https://doi.org/10.1017/S0950268815002290  PMID: 26493476 
  29. Nielsen TD, Kudahl AB, Østergaard S, Nielsen LR. Gross margin losses due to Salmonella Dublin infection in Danish dairy cattle herds estimated by simulation modelling. Prev Vet Med. 2013;111(1-2):51-62.  https://doi.org/10.1016/j.prevetmed.2013.03.011  PMID: 23628337 
  30. Spier SJ, Smith BP, Cullor JS, Olander HJ, Roden LD, Dilling GW. Persistent experimental Salmonella dublin intramammary infection in dairy cows. J Vet Intern Med. 1991;5(6):341-50.  https://doi.org/10.1111/j.1939-1676.1991.tb03148.x  PMID: 1779428 
  31. Uzzau S, Brown DJ, Wallis T, Rubino S, Leori G, Bernard S, et al. Host adapted serotypes of Salmonella enterica. Epidemiol Infect. 2000;125(2):229-55.  https://doi.org/10.1017/S0950268899004379  PMID: 11117946 
Disentangling a complex nationwide Salmonella Dublin outbreak associated with raw-milk cheese consumption, France, 2015 to 2016
<p class="citation"> <span>Citation style for this article:</span> <span class="meta-value authors"> <a href="/search?value1=Aymeric+Ung&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Ung Aymeric</a>, <a href="/search?value1=Amrish+Y.+Baidjoe&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Baidjoe Amrish Y.</a>, <a href="/search?value1=Dieter+Van+Cauteren&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Van Cauteren Dieter</a>, <a href="/search?value1=Nizar+Fawal&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Fawal Nizar</a>, <a href="/search?value1=Laetitia+Fabre&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Fabre Laetitia</a>, <a href="/search?value1=Caroline+Guerrisi&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Guerrisi Caroline</a>, <a href="/search?value1=Kostas+Danis&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Danis Kostas</a>, <a href="/search?value1=Anne+Morand&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Morand Anne</a>, <a href="/search?value1=Marie-Pierre+Donguy&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Donguy Marie-Pierre</a>, <a href="/search?value1=Etienne+Lucas&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Lucas Etienne</a>, <a href="/search?value1=Louise+Rossignol&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Rossignol Louise</a>, <a href="/search?value1=Sophie+Lef%C3%A8vre&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Lefèvre Sophie</a>, <a href="/search?value1=Marie-L%C3%A9one+Vignaud&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Vignaud Marie-Léone</a>, <a href="/search?value1=Sabrina+Cadel-Six&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Cadel-Six Sabrina</a>, <a href="/search?value1=Renaud+Lailler&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Lailler Renaud</a>, <a href="/search?value1=Nathalie+Jourdan-Da+Silva&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Jourdan-Da Silva Nathalie</a>, <a href="/search?value1=Simon+Le+Hello&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Le Hello Simon</a></span>. Disentangling a complex nationwide Salmonella Dublin outbreak associated with raw-milk cheese consumption, France, 2015 to 2016. <a href="/content/ecdc">Euro Surveill.</a> 2019;24(3):pii=1700703. <a href="https://doi.org/10.2807/1560-7917.ES.2019.24.3.1700703" title="doi link" target="_blank">https://doi.org/10.2807/1560-7917.ES.2019.24.3.1700703</a> <span class="ES_Article_citation"> <span class="generated">Received</span>: 06 Oct 2017;&nbsp;&nbsp; <span class="generated">Accepted</span>: 06 Jul 2018 </span> </p>
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