1887
  1. Home
  2. Eurosurveillance
  3. Volume 24, Issue 22, 30/May/2019
  4. Article
Rapid communication Open Access
Like 0
Download

Abstract

Since February 2019, over 160 (CT) cases testing negative or equivocal by Aptima Combo 2 (AC2) but positive by Aptima CT test run with Panther instruments occurred in Finland. The AC2 test targets chlamydial 23S rRNA while the CT test targets 16S rRNA. Sequencing of 10 strains revealed a nucleotide substitution in 23S rRNA. The significance of this for the failure of the AC2 test to detect the variant is not yet known.

© Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Addendum
An addendum has been published for this content:
Addendum for Euro Surveill. 2019;24(22)
Loading

Article metrics loading...

/content/10.2807/1560-7917.ES.2019.24.22.1900298
2019-05-30
2024-11-21
http://instance.metastore.ingenta.com/content/10.2807/1560-7917.ES.2019.24.22.1900298
Loading
Loading full text...

Full text loading...

/deliver/fulltext/eurosurveillance/24/22/eurosurv-24-22-1.html?itemId=/content/10.2807/1560-7917.ES.2019.24.22.1900298&mimeType=html&fmt=ahah

References

  1. Package insert Aptima Combo 2. https://www.hologic.com/sites/default/files/2019-04/502183EN-IFU-PI_007_01.pdf
  2. Boyadzhyan B, Yashina T, Yatabe JH, Patnaik M, Hill CS. Comparison of the APTIMA CT and GC assays with the APTIMA combo 2 assay, the Abbott LCx assay, and direct fluorescent-antibody and culture assays for detection of Chlamydia trachomatis and Neisseria gonorrhoeae. J Clin Microbiol. 2004;42(7):3089-93.  https://doi.org/10.1128/JCM.42.7.3089-3093.2004  PMID: 15243065 
  3. Package insert Aptima CT. https://www.hologic.com/sites/default/files/2019-04/502184EN-IFU-PI_007_01.pdf
  4. Korhonen S, Hiltunen-Back E, Puolakkainen M. Genotyping of Chlamydia trachomatis in rectal and pharyngeal specimens: identification of LGV genotypes in Finland. Sex Transm Infect. 2012;88(6):465-9.  https://doi.org/10.1136/sextrans-2011-050458  PMID: 22517888 
  5. Hadfield J, Harris SR, Seth-Smith HMB, Parmar S, Andersson P, Giffard PM, et al. Comprehensive global genome dynamics of Chlamydia trachomatis show ancient diversification followed by contemporary mixing and recent lineage expansion. Genome Res. 2017;27(7):1220-9.  https://doi.org/10.1101/gr.212647.116  PMID: 28588068 
  6. Ripa T, Nilsson P. A variant of Chlamydia trachomatis with deletion in cryptic plasmid: implications for use of PCR diagnostic tests. Euro Surveill. 2006;11(11):3076. PMID: 17213548 
  7. Herrmann B, Törner A, Low N, Klint M, Nilsson A, Velicko I, et al. Emergence and spread of Chlamydia trachomatis variant, Sweden. Emerg Infect Dis. 2008;14(9):1462-5.  https://doi.org/10.3201/eid1409.080153  PMID: 18760021 
  8. Unemo M, Clarke IN. The Swedish new variant of Chlamydia trachomatis. Curr Opin Infect Dis. 2011;24(1):62-9.  https://doi.org/10.1097/QCO.0b013e32834204d5  PMID: 21157332 
  9. Nelson HD, Zakher B, Cantor A, Deagas M, Pappas M. Screening for gonorrhea and chlamydia: systematic review to update the U.S. Preventive Services Task Force Recommendations. Evidence Synthesis, No. 115. Rockville, MD: Agency for Healthcare Research and Quality; September 2014.
Chlamydia trachomatis samples testing falsely negative in the Aptima Combo 2 test in Finland, 2019
<p class="citation"> <span>Citation style for this article:</span> <span class="meta-value authors"> <a href="/search?value1=Kaisu+Rantakokko-Jalava&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Rantakokko-Jalava Kaisu</a>, <a href="/search?value1=Kati+Hokynar&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Hokynar Kati</a>, <a href="/search?value1=Niina+Hieta&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Hieta Niina</a>, <a href="/search?value1=Anniina+Keskitalo&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Keskitalo Anniina</a>, <a href="/search?value1=Pia+Jokela&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Jokela Pia</a>, <a href="/search?value1=Anna+Muotiala&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Muotiala Anna</a>, <a href="/search?value1=T.+Sakari+Jokiranta&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Jokiranta T. Sakari</a>, <a href="/search?value1=Rutta+Kuusela&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Kuusela Rutta</a>, <a href="/search?value1=Hannu+Sarkkinen&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Sarkkinen Hannu</a>, <a href="/search?value1=Janne+Aittoniemi&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Aittoniemi Janne</a>, <a href="/search?value1=Tytti+Vuorinen&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Vuorinen Tytti</a>, <a href="/search?value1=Antti+J+Hakanen&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Hakanen Antti J</a>, <a href="/search?value1=Mirja+Puolakkainen&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Puolakkainen Mirja</a></span>. Chlamydia trachomatis samples testing falsely negative in the Aptima Combo 2 test in Finland, 2019. <a href="/content/ecdc">Euro Surveill.</a> 2019;24(22):pii=1900298. <a href="https://doi.org/10.2807/1560-7917.ES.2019.24.22.1900298" title="doi link" target="_blank">https://doi.org/10.2807/1560-7917.ES.2019.24.22.1900298</a> <span class="ES_Article_citation"> <span class="generated">Received</span>: 14 May 2019;&nbsp;&nbsp; <span class="generated">Accepted</span>: 30 May 2019 </span> </p>
/content/10.2807/1560-7917.ES.2019.24.22.1900298
/content/10.2807/1560-7917.ES.2019.24.22.1900298
Loading

Data & Media loading...

Submit comment
Close
Comment moderation successfully completed
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error