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Analysis of OXA-204 carbapenemase-producing Enterobacteriaceae reveals possible endoscopy-associated transmission, France, 2012 to 2014
- Anaïs Potron1,2 , Sandrine Bernabeu1,3,4,5 , Gaëlle Cuzon1,3,4,5 , Valérie Pontiès6 , Hervé Blanchard7 , Elise Seringe7 , Thierry Naas1,3,4,5 , Patrice Nordmann8,9,10,11 , Laurent Dortet1,3,4,5
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View Affiliations Hide AffiliationsAffiliations: 1 National Reference Centre for Antibiotic Resistance, (division of carbapenemase-producing Enterobacteriaceae), Le Kremlin-Bicêtre, France 2 Department of Bacteriology, University Hospital of Besançon, Université of Franche-Comté, Besançon, France 3 Bacteriology-Hygiene Unit, Assistance Publique/Hôpitaux de Paris, Bicêtre Hospital, Le Kremlin-Bicêtre, France 4 EA7361 “Structure, Dynamic, Function and Expression of Broad Spectrum beta-Lactamases”, Paris-Sud University, LabEx Lermit, Faculty of Medicine, Le Kremlin-Bicêtre, France 5 Joint Research Unit EERA “Evolution and Ecology of Resistance to Antibiotics,” Institut Pasteur-APHP-Université Paris-Sud, Paris, France 6 Santé Publique France, The French Public Health Agency, Saint-Maurice, France 7 Regional Coordinating Centre for Nosocomial Infection Control (C-CLIN Paris Nord), Paris, France. 8 Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Department of Medicine, University of Fribourg, Fribourg, Switzerland 9 Institut National de la Santé et de la Recherche Médicale (INSERM) European Unit (LEA Paris, IAME, France), University of Fribourg, Switzerland 10 National Reference Centre for Emerging Antibiotic Resistance, Fribourg, Switzerland 11 Institute for Microbiology, University hospital and University of Lausanne, Lausanne, SwitzerlandLaurent Dortetlaurent.dortet aphp.fr
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Citation style for this article: Potron Anaïs, Bernabeu Sandrine, Cuzon Gaëlle, Pontiès Valérie, Blanchard Hervé, Seringe Elise, Naas Thierry, Nordmann Patrice, Dortet Laurent. Analysis of OXA-204 carbapenemase-producing Enterobacteriaceae reveals possible endoscopy-associated transmission, France, 2012 to 2014. Euro Surveill. 2017;22(49):pii=17-00048. https://doi.org/10.2807/1560-7917.ES.2017.22.49.17-00048 Received: 16 Jan 2017; Accepted: 23 May 2017
Abstract
OXA-48-like beta-lactamase producing bacteria are now endemic in several European and Mediterranean countries. Among this carbapenemase family, the OXA-48 and OXA-181 variants predominate, whereas other variants such as OXA-204 are rarely reported. Here, we report the molecular epidemiology of a collection of OXA-204-positive enterobacterial isolates (n = 29) recovered in France between October 2012 and May 2014. This study describes the first outbreak of OXA-204-producing Enterobacteriaceae in Europe, involving 12 isolates of an ST90 Escherichia coli clone and nine isolates of an ST147 Klebsiella pneumoniae clone. All isolates co-produced the cephalosporinase CMY-4, and 60% of them co-produced the extended-spectrum beta-lactamase CTX-M-15. The blaOXA-204 gene was located on a 150-kb IncA/C plasmid, isolated from various enterobacterial species in the same patient, indicating a high conjugative ability of this genetic vehicle.
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