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Abstract

We report the isolation of infectious Zika virus (ZIKV) in cell culture from the saliva of a patient who developed a febrile illness after returning from the Dominican Republic to Italy, in January 2016. The patient had prolonged shedding of viral RNA in saliva and urine, at higher load than in blood, for up to 29 days after symptom onset. Sequencing of ZIKV genome showed relatedness with strains from Latin America.

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/content/10.2807/1560-7917.ES.2016.21.10.30159
2016-03-10
2024-11-19
http://instance.metastore.ingenta.com/content/10.2807/1560-7917.ES.2016.21.10.30159
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References

  1. Santiago GA, Vergne E, Quiles Y, Cosme J, Vazquez J, Medina JF, et al. Analytical and clinical performance of the CDC real time RT-PCR assay for detection and typing of dengue virus. PLoS Negl Trop Dis. 2013;7(7):e2311. Available from:  https://doi.org/10.1371/journal.pntd.0002311  PMID: 23875046 
  2. Pastorino B, Bessaud M, Grandadam M, Murri S, Tolou HJ, Peyrefitte CN. Development of a TaqMan RT-PCR assay without RNA extraction step for the detection and quantification of African Chikungunya viruses. J Virol Methods. 2005;124(1-2):65-71. Available from:  https://doi.org/10.1016/j.jviromet.2004.11.002  PMID: 15664052 
  3. Lanciotti RS, Kosoy OL, Laven JJ, Velez JO, Lambert AJ, Johnson AJ, et al. Genetic and serologic properties of Zika virus associated with an epidemic, Yap State, Micronesia, 2007. Emerg Infect Dis. 2008;14(8):1232-9. Available from:  https://doi.org/10.3201/eid1408.080287  PMID: 18680646 
  4. Barzon L, Pacenti M, Franchin E, Squarzon L, Sinigaglia A, Ulbert S, et al. Isolation of West Nile virus from urine samples of patients with acute infection. J Clin Microbiol. 2014;52(9):3411-3. Available from:  https://doi.org/10.1128/JCM.01328-14  PMID: 24951801 
  5. Duffy MR, Chen TH, Hancock WT, Powers AM, Kool JL, Lanciotti RS, et al. Zika virus outbreak on Yap Island, Federated States of Micronesia. N Engl J Med. 2009;360(24):2536-43. Available from:  https://doi.org/10.1056/NEJMoa0805715  PMID: 19516034 
  6. Cao-Lormeau VM, Roche C, Teissier A, Robin E, Berry AL, Mallet HP, et al. Zika virus, French Polynesia, South Pacific, 2013. Emerg Infect Dis. 2014;20(6):1085-6. Available from:  https://doi.org/10.3201/eid2006.140138  PMID: 24856001 
  7. Roth A, Mercier A, Lepers C, Hoy D, Duituturaga S, Benyon E, et al. Concurrent outbreaks of dengue, chikungunya and Zika virus infections - an unprecedented epidemic wave of mosquito-borne viruses in the Pacific 2012-2014. Euro Surveill. 2014;19(41):20929. Available from:  https://doi.org/10.2807/1560-7917.ES2014.19.41.20929  PMID: 25345518 
  8. Zammarchi L, Tappe D, Fortuna C, Remoli ME, Günther S, Venturi G, et al. Zika virus infection in a traveller returning to Europe from Brazil, March 2015. Euro Surveill. 2015;20(23):21153. Available from:  https://doi.org/10.2807/1560-7917.ES2015.20.23.21153  PMID: 26084316 
  9. World Health Organization. Zika virus infection: global update on epidemiology and potentially associated clinical manifestations. Wkly Epidemiol Rec. 2016;91(7):73-81. PMID: 26897760 
  10. Calvet G, Aguiar RS, Melo AS, Sampaio SA, de Filippis I, Fabri A, et al. Detection and sequencing of Zika virus from amniotic fluid of fetuses with microcephaly in Brazil: a case study. Lancet Infect Dis. 2016; pii: S1473-3099(16)00095-5.
  11. Mlakar J, Korva M, Tul N, Popović M, Poljšak-Prijatelj M, Mraz J, et al. Zika virus associated with microcephaly. N Engl J Med. 2016. [Epub ahead of print].
  12. Martines RB, Bhatnagar J, Keating MK, Silva-Flannery L, Muehlenbachs A, Gary J, et al. Notes from the Field: Evidence of Zika virus infection in brain and placental tissues from two congenitally infected newborns and two fetal losses - Brazil, 2015. MMWR Morb Mortal Wkly Rep. 2016;65(6):159-60. Available from:  https://doi.org/10.15585/mmwr.mm6506e1  PMID: 26890059 
  13. Cao-Lormeau VM, Blake A, Mons S, Lastère S, Roche C, Vanhomwegen J, et al. Guillain-Barré Syndrome outbreak associated with Zika virus infection in French Polynesia: a case-control study. Lancet. 2016;S0140-6736(16)00562-6. [Epub ahead of print]. PMID: 26948433 
  14. Li MI, Wong PS, Ng LC, Tan CH. Oral susceptibility of Singapore Aedes (Stegomyia) aegypti (Linnaeus) to Zika virus. PLoS Negl Trop Dis. 2012;6(8):e1792.
  15. Grard G, Caron M, Mombo IM, Nkoghe D, Mboui Ondo S, Jiolle D, et al. Zika virus in Gabon (Central Africa)--2007: a new threat from Aedes albopictus? PLoS Negl Trop Dis. 2014;8(2):e2681. Available from:  https://doi.org/10.1371/journal.pntd.0002681  PMID: 24516683 
  16. Wong PS, Li MZ, Chong CS, Ng LC, Tan CH. Aedes (Stegomyia) albopictus (Skuse): a potential vector of Zika virus in Singapore. PLoS Negl Trop Dis. 2013;7(8):e2348. Available from:  https://doi.org/10.1371/journal.pntd.0002348  PMID: 23936579 
  17. Gobbi F, Capelli G, Angheben A, Giobbia M, Conforto M, Franzetti M, et al. Summer Fever Study Group. Human and entomological surveillance of West Nile fever, dengue and chikungunya in Veneto Region, Italy, 2010-2012. BMC Infect Dis. 2014;14(1):60. Available from:  https://doi.org/10.1186/1471-2334-14-60  PMID: 24499011 
  18. Rezza G, Nicoletti L, Angelini R, Romi R, Finarelli AC, Panning M, et al. CHIKV study group. Infection with chikungunya virus in Italy: an outbreak in a temperate region. Lancet. 2007;370(9602):1840-6. Available from:  https://doi.org/10.1016/S0140-6736(07)61779-6  PMID: 18061059 
  19. Besnard M, Lastere S, Teissier A, Cao-Lormeau V, Musso D. Evidence of perinatal transmission of Zika virus, French Polynesia, December 2013 and February 2014. Euro Surveill. 2014;19(13):20751. https://doi.org/10.2807/1560-7917.ES2014.19.13.20751  PMID: 24721538 
  20. Musso D, Nhan T, Robin E, Roche C, Bierlaire D, Zisou K, et al. Potential for Zika virus transmission through blood transfusion demonstrated during an outbreak in French Polynesia, November 2013 to February 2014. Euro Surveill. 2014;19(14):20761. Available from:  https://doi.org/10.2807/1560-7917.ES2014.19.14.20761  PMID: 24739982 
  21. Centers for Disease Control and Prevention (CDC). HAN Priority Professional and Media Partners Update. Update: Interim Guidelines for Prevention of Sexual Transmission of Zika Virus — United States, 2016. 23 Feb 2016. Available from: http://content.govdelivery.com/accounts/USCDC/bulletins/1383154.
  22. Musso D, Roche C, Robin E, Nhan T, Teissier A, Cao-Lormeau VM. Potential sexual transmission of Zika virus. Emerg Infect Dis. 2015;21(2):359-61. Available from:  https://doi.org/10.3201/eid2102.141363  PMID: 25625872 
  23. Hills SL, Russell K, Hennessey M, Williams C, Oster AM, Fischer M, et al. Transmission of Zika virus through sexual contact with travelers to areas of ongoing transmission — Continental United States, 2016. MMWR Morb Mortal Wkly Rep. 2016;65(8):215-6. Available from:  https://doi.org/10.15585/mmwr.mm6508e2  PMID: 26937739 
  24. Foy BD, Kobylinski KC, Chilson Foy JL, Blitvich BJ, Travassos da Rosa A, Haddow AD, et al. Probable non-vector-borne transmission of Zika virus, Colorado, USA. Emerg Infect Dis. 2011;17(5):880-2. Available from:  https://doi.org/10.3201/eid1705.101939  PMID: 21529401 
  25. Musso D, Roche C, Nhan TX, Robin E, Teissier A, Cao-Lormeau VM. Detection of Zika virus in saliva. J Clin Virol. 2015;68:53-5. https://doi.org/10.1016/j.jcv.2015.04.021  PMID: 26071336 
  26. Gourinat AC, O’Connor O, Calvez E, Goarant C, Dupont-Rouzeyrol M. Detection of Zika virus in urine. Emerg Infect Dis. 2015;21(1):84-6. Available from:  https://doi.org/10.3201/eid2101.140894  PMID: 25530324 
  27. de M Campos R. Cirne-Santos C, Meira GL, Santos LL, de Meneses MD, Friedrich J, et al. Prolonged detection of Zika virus RNA in urine samples during the ongoing Zika virus epidemic in Brazil. J Clin Virol. 2016;77:69-70.
  28. Andries AC, Duong V, Ly S, Cappelle J, Kim KS, Lorn Try P, et al. Value of routine dengue diagnostic tests in urine and saliva specimens. PLoS Negl Trop Dis. 2015;9(9):e0004100. Available from:  https://doi.org/10.1371/journal.pntd.0004100  PMID: 26406240 
  29. Korhonen EM, Huhtamo E, Virtala AM, Kantele A, Vapalahti O. Approach to non-invasive sampling in dengue diagnostics: exploring virus and NS1 antigen detection in saliva and urine of travelers with dengue. J Clin Virol. 2014;61(3):353-8. Available from:  https://doi.org/10.1016/j.jcv.2014.08.021  PMID: 25242312 
  30. Barzon L, Pacenti M, Franchin E, Pagni S, Martello T, Cattai M, et al. Excretion of West Nile virus in urine during acute infection. J Infect Dis. 2013;208(7):1086-92. Available from:  https://doi.org/10.1093/infdis/jit290  PMID: 23821721 
  31. Barzon L, Pacenti M, Franchin E, Squarzon L, Lavezzo E, Toppo S, et al. Novel West Nile virus lineage 1a full genome sequences from human cases of infection in north-eastern Italy, 2011. Clin Microbiol Infect. 2012;18(12):E541-4. Available from:  https://doi.org/10.1111/1469-0691.12001  PMID: 23004685 
  32. Fonseca K, Meatherall B, Zarra D, Drebot M, MacDonald J, Pabbaraju K, et al. First case of Zika virus infection in a returning Canadian traveler. Am J Trop Med Hyg. 2014;91(5):1035-8. Available from:  https://doi.org/10.4269/ajtmh.14-0151  PMID: 25294619 
  33. Dupount-Rouzeyrol M, Biron A, O’Connor O, Huguan E, Descloux E. Infectious Zika viral particles in breastmilk. Lancet. 2016; S0140-6736(16)00624-3.
  34. Leung GH, Baird RW, Druce J, Anstey NM. Zika virus infection in Australia following a monkey bite in Indonesia. Southeast Asian J Trop Med Public Health. 2015;46(3):460-4. PMID: 26521519 
  35. Gardner J, Rudd PA, Prow NA, Belarbi E, Roques P, Larcher T, et al. Infectious chikungunya virus in the saliva of mice, monkeys and humans. PLoS One. 2015;10(10):e0139481. Available from:  https://doi.org/10.1371/journal.pone.0139481  PMID: 26447467 
  36. Tamura K, Nei M. Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol Biol Evol. 1993;10(3):512-26. PMID: 8336541 
  37. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Mol Biol Evol. 2013;30(12):2725-9. Available from:  https://doi.org/10.1093/molbev/mst197  PMID: 24132122 
Isolation of infectious Zika virus from saliva and prolonged viral RNA shedding in a traveller returning from the Dominican Republic to Italy, January 2016
<p class="citation"> <span>Citation style for this article:</span> <span class="meta-value authors"> <a href="/search?value1=Luisa+Barzon&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Barzon Luisa</a>, <a href="/search?value1=Monia+Pacenti&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Pacenti Monia</a>, <a href="/search?value1=Alessandro+Berto&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Berto Alessandro</a>, <a href="/search?value1=Alessandro+Sinigaglia&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Sinigaglia Alessandro</a>, <a href="/search?value1=Elisa+Franchin&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Franchin Elisa</a>, <a href="/search?value1=Enrico+Lavezzo&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Lavezzo Enrico</a>, <a href="/search?value1=Pierluigi+Brugnaro&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Brugnaro Pierluigi</a>, <a href="/search?value1=Giorgio+Pal%C3%B9&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Palù Giorgio</a></span>. Isolation of infectious Zika virus from saliva and prolonged viral RNA shedding in a traveller returning from the Dominican Republic to Italy, January 2016. <a href="/content/ecdc">Euro Surveill.</a> 2016;21(10):pii=30159. <a href="https://doi.org/10.2807/1560-7917.ES.2016.21.10.30159" title="doi link" target="_blank">https://doi.org/10.2807/1560-7917.ES.2016.21.10.30159</a> <span class="ES_Article_citation"> <span class="generated">Received</span>: 03 Mar 2016;&nbsp;&nbsp; <span class="generated">Accepted</span>: 10 Mar 2016 </span> </p>
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