Rapid communication Open Access
http://instance.metastore.ingenta.com/content/10.2807/1560-7917.ES.2017.22.31.30586
Like 0
Access full text
Article
-
Co-occurrence of colistin-resistance genes mcr-1 and mcr-3 among multidrug-resistant Escherichia coli isolated from cattle, Spain, September 2015
- Marta Hernández1,2,3 , M Rocío Iglesias3,4 , David Rodríguez-Lázaro3,5 , Alejandro Gallardo4 , Narciso Quijada1 , Pedro Miguela-Villoldo6 , Maria Jorge Campos7 , Segundo Píriz8 , Gema López-Orozco9 , Cristina de Frutos10 , José Luis Sáez9 , María Ugarte-Ruiz6 , Lucas Domínguez6,11 , Alberto Quesada4,12
-
View Affiliations Hide AffiliationsAffiliations: 1 Laboratorio de Biología Molecular y Microbiología, Instituto Tecnológico Agrario de Castilla y León, Valladolid, Spain 2 Departamento de Ingeniería Agrícola y Forestal, Tecnología de los Alimentos, Escuela Técnica Superior de Ingenierías Agrarias, Universidad de Valladolid, Palencia, Spain 3 These authors contributed equally to the manuscript 4 Departamento de Bioquímica, Biología Molecular y Genética, Facultad de Veterinaria, Universidad de Extremadura, Cáceres, Spain 5 Área de Microbiología, Departamento de Biotecnología y Ciencia de los Alimentos, Universidad de Burgos, Burgos, Spain 6 VISAVET Health Surveillance Centre, Universidad Complutense, Madrid, Spain 7 MARE - Marine and Environmental Sciences Centre, Instituto Politécnico de Leiria, Peniche, Portugal 8 Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad de Extremadura, Cáceres, Spain 9 Subdirección General de Sanidad e Higiene Animal y Trazabilidad, Ministerio de Agricultura y Pesca, Alimentación y Medio Ambiente, Madrid, Spain 10 Laboratorio Central de Veterinaria, Ministerio de Agricultura y Pesca, Alimentación y Medio Ambiente, Algete, Spain 11 Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain. 12 INBIO G+C, Universidad de Extremadura, Cáceres, SpainCorrespondence:María Ugarte-Ruizmaria.ugarte visavet.ucm.es
-
View Citation Hide Citation
Citation style for this article: Hernández Marta, Iglesias M Rocío, Rodríguez-Lázaro David, Gallardo Alejandro, Quijada Narciso, Miguela-Villoldo Pedro, Campos Maria Jorge, Píriz Segundo, López-Orozco Gema, de Frutos Cristina, Sáez José Luis, Ugarte-Ruiz María, Domínguez Lucas, Quesada Alberto. Co-occurrence of colistin-resistance genes mcr-1 and mcr-3 among multidrug-resistant Escherichia coli isolated from cattle, Spain, September 2015. Euro Surveill. 2017;22(31):pii=30586. https://doi.org/10.2807/1560-7917.ES.2017.22.31.30586 Received: 20 Jul 2017; Accepted: 02 Aug 2017
Abstract
Colistin resistance genes mcr-3 and mcr-1 have been detected in an Escherichia coli isolate from cattle faeces in a Spanish slaughterhouse in 2015. The sequences of both genes hybridised to same plasmid band of ca 250 kb, although colistin resistance was non-mobilisable. The isolate was producing extended-spectrum beta-lactamases and belonged to serotype O9:H10 and sequence type ST533. Here we report an mcr-3 gene detected in Europe following earlier reports from Asia and the United States.
Article metrics loading...
/content/10.2807/1560-7917.ES.2017.22.31.30586
2017-08-03
2024-11-22
http://instance.metastore.ingenta.com/content/10.2807/1560-7917.ES.2017.22.31.30586
Full text loading...
/deliver/fulltext/eurosurveillance/22/31/eurosurv-22-31-4.html?itemId=/content/10.2807/1560-7917.ES.2017.22.31.30586&mimeType=html&fmt=ahah
References
-
Yin W, Li H, Shen Y, Liu Z, Wang S, Shen Z, et al. Novel plasmid-mediated colistin resistance gene mcr-3 in Escherichia coli. MBio. 2017;8(3):e00543-17. https://doi.org/10.1128/mBio.00543-17 PMID: 28655818
-
European Commission. Commission implementing decision 2013/652/EU on the monitoring and reporting of antimicrobial resistance in zoonotic and commensal bacteria. Official Journal of the European Union. Luxembourg: Publications Office of the European Union. 14.11.2013:L 303. Available from: http://eur-lex.europa.eu/legal-content/EN/TXT/PDF/?uri=OJ:L:2013:303:FULL&from=EN
-
Hasman H, Agersø Y, Hendriksen R, Cavaco LM, Guerra-Roman B. Isolation of ESBL-, AmpC- and carbapenemase-producing E. coli from caecal samples. Laboratory Protocol. Version 4. Lyngby: DTU Foof; Jan 2017. Available from: http://www.eurl-ar.eu/data/images/protocols/esbl_ampc_cpeprotocol_version_caecal_january2017_version4.pdf
-
Liu YY, Wang Y, Walsh TR, Yi LX, Zhang R, Spencer J, et al. Emergence of plasmid-mediated colistin resistance mechanism MCR-1 in animals and human beings in China: a microbiological and molecular biological study. Lancet Infect Dis. 2016;16(2):161-8. https://doi.org/10.1016/S1473-3099(15)00424-7 PMID: 26603172
-
Xavier BB, Lammens C, Ruhal R, Kumar-Singh S, Butaye P, Goossens H, et al. Identification of a novel plasmid-mediated colistin-resistance gene, mcr-2, in Escherichia coli, Belgium, June 2016. Euro Surveill. 2016;21(27):30280. https://doi.org/10.2807/1560-7917.ES.2016.21.27.30280 PMID: 27416987
-
International Organization for Standardization (ISO). Clinical laboratory testing and in vitro diagnostic test systems -- Susceptibility testing of infectious agents and evaluation of performance of antimicrobial susceptibility test devices -- Part 1: Reference method for testing the in vitro activity of antimicrobial agents against rapidly growing aerobic bacteria involved in infectious diseases. ISO 20776-1:2006. Geneva: ISO; 2006. Available from: https://www.iso.org/standard/41630.html
-
European Committee on Antimicrobial Susceptibility Testing (EUCAST). Antimicrobial wild type distributions of microorganisms. Växjö: EUCAST. [Accessed: July 2017]. Available from:https://mic.eucast.org/Eucast2/SearchController/search.jsp?action=init
-
Bankevich A, Nurk S, Antipov D, Gurevich AA, Dvorkin M, Kulikov AS, et al. SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing. J Comput Biol. 2012;19(5):455-77. https://doi.org/10.1089/cmb.2012.0021 PMID: 22506599
-
Seemann T. Prokka: rapid prokaryotic genome annotation. Bioinformatics. 2014;30(14):2068-9. https://doi.org/10.1093/bioinformatics/btu153 PMID: 24642063
-
Zhang Z, Schwartz S, Wagner L, Miller W. A greedy algorithm for aligning DNA sequences. J Comput Biol. 2000;7(1-2):203-14. https://doi.org/10.1089/10665270050081478 PMID: 10890397
-
Zankari E, Hasman H, Cosentino S, Vestergaard M, Rasmussen S, Lund O, et al. Identification of acquired antimicrobial resistance genes. J Antimicrob Chemother. 2012;67(11):2640-4. https://doi.org/10.1093/jac/dks261 PMID: 22782487
-
Carattoli A, Zankari E, García-Fernández A, Voldby Larsen M, Lund O, Villa L, et al. In silico detection and typing of plasmids using PlasmidFinder and plasmid multilocus sequence typing. Antimicrob Agents Chemother. 2014;58(7):3895-903. https://doi.org/10.1128/AAC.02412-14 PMID: 24777092
-
Li R, Xie M, Zhang J, Yang Z, Liu L, Liu X, et al. Genetic characterization of mcr-1-bearing plasmids to depict molecular mechanisms underlying dissemination of the colistin resistance determinant. J Antimicrob Chemother. 2017;72(2):393-401. https://doi.org/10.1093/jac/dkw411 PMID: 28073961
-
NCBI Resource Coordinators. Database Resources of the National Center for Biotechnology Information. Nucleic Acids Res. 2017;45(D1):D12-7. https://doi.org/10.1093/nar/gkw1071 PMID: 27899561
-
Sánchez-Benito R, Iglesias MR, Quijada NM, Campos MJ, Ugarte-Ruiz M, Hernández M, et al. Escherichia coli ST167 carrying plasmid mobilisable mcr-1 and blaCTX-M-15 resistance determinants isolated from a human respiratory infection. Int J Antimicrob Agents. 2017;S0924-8579(17)30181-4. (Forthcoming). PMID: 28599866
-
Quesada A, Ugarte-Ruiz M, Iglesias MR, Porrero MC, Martínez R, Florez-Cuadrado D, et al. Detection of plasmid mediated colistin resistance (MCR-1) in Escherichia coli and Salmonella enterica isolated from poultry and swine in Spain. Res Vet Sci. 2016;105:134-5. https://doi.org/10.1016/j.rvsc.2016.02.003 PMID: 27033921
-
Nonaka L, Maruyama F, Onishi Y, Kobayashi T, Ogura Y, Hayashi T, et al. Various pAQU plasmids possibly contribute to disseminate tetracycline resistance gene tet(M) among marine bacterial community. Front Microbiol. 2014;5(5):152. PMID: 24860553
Co-occurrence of colistin-resistance genes mcr-1 and mcr-3 among multidrug-resistant Escherichia coli isolated from cattle, Spain, September 2015
<p class="citation">
<span>Citation style for this article:</span>
<span class="meta-value authors">
<a href="/search?value1=Marta+Hern%C3%A1ndez&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Hernández Marta</a>,
<a href="/search?value1=M+Roc%C3%ADo+Iglesias&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Iglesias M Rocío</a>,
<a href="/search?value1=David+Rodr%C3%ADguez-L%C3%A1zaro&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Rodríguez-Lázaro David</a>,
<a href="/search?value1=Alejandro+Gallardo&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Gallardo Alejandro</a>,
<a href="/search?value1=Narciso+Quijada&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Quijada Narciso</a>,
<a href="/search?value1=Pedro+Miguela-Villoldo&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Miguela-Villoldo Pedro</a>,
<a href="/search?value1=Maria+Jorge+Campos&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Campos Maria Jorge</a>,
<a href="/search?value1=Segundo+P%C3%ADriz&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Píriz Segundo</a>,
<a href="/search?value1=Gema+L%C3%B3pez-Orozco&option1=author&noRedirect=true" class="nonDisambigAuthorLink">López-Orozco Gema</a>,
<a href="/search?value1=Cristina+de+Frutos&option1=author&noRedirect=true" class="nonDisambigAuthorLink">de Frutos Cristina</a>,
<a href="/search?value1=Jos%C3%A9+Luis+S%C3%A1ez&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Sáez José Luis</a>,
<a href="/search?value1=Mar%C3%ADa+Ugarte-Ruiz&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Ugarte-Ruiz María</a>,
<a href="/search?value1=Lucas+Dom%C3%ADnguez&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Domínguez Lucas</a>,
<a href="/search?value1=Alberto+Quesada&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Quesada Alberto</a></span>.
Co-occurrence of colistin-resistance genes mcr-1 and mcr-3 among multidrug-resistant Escherichia coli isolated from cattle, Spain, September 2015.
<a href="/content/ecdc">Euro Surveill.</a> 2017;22(31):pii=30586.
<a href="https://doi.org/10.2807/1560-7917.ES.2017.22.31.30586" title="doi link" target="_blank">https://doi.org/10.2807/1560-7917.ES.2017.22.31.30586</a>
<span class="ES_Article_citation">
<span class="generated">Received</span>: 20 Jul 2017;
<span class="generated">Accepted</span>: 02 Aug 2017
</span>
</p>
/content/10.2807/1560-7917.ES.2017.22.31.30586
/content/10.2807/1560-7917.ES.2017.22.31.30586
Data & Media loading...
Submit comment
Close
Comment moderation successfully completed