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Abstract

The need for timely establishment of diagnostic assays arose when Germany was confronted with the first travel-associated outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in Europe. We describe our laboratory experiences during a large contact tracing investigation, comparing previously published real-time RT-PCR assays in different PCR systems and a commercial kit. We found that assay performance using the same primers and probes with different PCR systems varied and the commercial kit performed well.

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/content/10.2807/1560-7917.ES.2020.25.9.2000173
2020-03-05
2024-12-22
/content/10.2807/1560-7917.ES.2020.25.9.2000173
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Rapid establishment of laboratory diagnostics for the novel coronavirus SARS-CoV-2 in Bavaria, Germany, February 2020
<p class="citation"> <span>Citation style for this article:</span> <span class="meta-value authors"> <a href="/search?value1=Regina+Konrad&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Konrad Regina</a>, <a href="/search?value1=Ute+Eberle&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Eberle Ute</a>, <a href="/search?value1=Alexandra+Dangel&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Dangel Alexandra</a>, <a href="/search?value1=Bianca+Treis&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Treis Bianca</a>, <a href="/search?value1=Anja+Berger&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Berger Anja</a>, <a href="/search?value1=Katja+Bengs&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Bengs Katja</a>, <a href="/search?value1=Volker+Fingerle&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Fingerle Volker</a>, <a href="/search?value1=Bernhard+Liebl&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Liebl Bernhard</a>, <a href="/search?value1=Nikolaus+Ackermann&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Ackermann Nikolaus</a>, <a href="/search?value1=Andreas+Sing&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Sing Andreas</a></span>. Rapid establishment of laboratory diagnostics for the novel coronavirus SARS-CoV-2 in Bavaria, Germany, February 2020. <a href="/content/ecdc">Euro Surveill.</a> 2020;25(9):pii=2000173. <a href="https://doi.org/10.2807/1560-7917.ES.2020.25.9.2000173" title="doi link" target="_blank">https://doi.org/10.2807/1560-7917.ES.2020.25.9.2000173</a> <span class="ES_Article_citation"> <span class="generated">Received</span>: 21 Feb 2020;&nbsp;&nbsp; <span class="generated">Accepted</span>: 04 Mar 2020 </span> </p>
/content/10.2807/1560-7917.ES.2020.25.9.2000173
/content/10.2807/1560-7917.ES.2020.25.9.2000173
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