1887
  1. Home
  2. Eurosurveillance
  3. Volume 22, Issue 39, 28/Sep/2017
  4. Article
Research article Open Access
Like 0
Download

Abstract

The recently discovered colistin resistance-encoding element, , adds to the list of mobile resistance genes whose products rapidly erode the antimicrobial efficacy of not only the commonly used antibiotics, but also the last line agents of carbapenems and colistin. The relative prevalence of -bearing strains in various ecological niches including 1,371 food samples, 480 animal faecal samples, 150 human faecal samples and 34 water samples was surveyed using a novel in-house method. Bacteria bearing were commonly detected in water (71% of samples), animal faeces (51%), food products (36%), and exhibited stable carriage in 28% of human subjects surveyed. Such strains, which exhibited variable antibiotic susceptibility profiles, belonged to various Enterobacteriaceae species, with being the most dominant in each specimen type. The gene was detectable in the chromosome as well as plasmids of various sizes. Among these, two conjugative plasmids of sizes ca 33 and ca 60 kb were found to be the key vectors that mediated transmission in organisms residing in various ecological niches. The high carriage rate in humans found in this study highlights the importance of continued vigilance, careful antibiotic stewardship, and the development of new antimicrobials.

© Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Loading

Article metrics loading...

/content/10.2807/1560-7917.ES.2017.22.39.17-00206
2017-09-28
2024-04-20
http://instance.metastore.ingenta.com/content/10.2807/1560-7917.ES.2017.22.39.17-00206
Loading
Loading full text...

Full text loading...

/deliver/fulltext/eurosurveillance/22/39/eurosurv-22-39-6.html?itemId=/content/10.2807/1560-7917.ES.2017.22.39.17-00206&mimeType=html&fmt=ahah

References

  1. Centers for Disease Control and Prevention (CDC). Antibiotic Resistance Threats in the United States, 2013.Atlanta: CDC; 2013. Available from: https://www.cdc.gov/drugresistance/threat-report-2013/index.html
  2. European Centre for Disease Control Prevention and Control (ECDC). Rapid risk assessment: Carbapenem-resitant Enterobacteriaceae – 8 April 2016. Stockholm: ECDC; 2016. Available from: https://ecdc.europa.eu/sites/portal/files/media/en/publications/Publications/carbapenem-resistant-enterobacteriaceae-risk-assessment-april-2016.pdf
  3. Miller AK, Brannon MK, Stevens L, Johansen HK, Selgrade SE, Miller SI, et al. PhoQ mutations promote lipid A modification and polymyxin resistance of Pseudomonas aeruginosa found in colistin-treated cystic fibrosis patients. Antimicrob Agents Chemother. 2011;55(12):5761-9.  https://doi.org/10.1128/AAC.05391-11  PMID: 21968359 
  4. Beceiro A, Moreno A, Fernández N, Vallejo JA, Aranda J, Adler B, et al. Biological cost of different mechanisms of colistin resistance and their impact on virulence in Acinetobacter baumannii. Antimicrob Agents Chemother. 2014;58(1):518-26.  https://doi.org/10.1128/AAC.01597-13  PMID: 24189257 
  5. Liu YY, Wang Y, Walsh TR, Yi LX, Zhang R, Spencer J, et al. Emergence of plasmid-mediated colistin resistance mechanism MCR-1 in animals and human beings in China: a microbiological and molecular biological study. Lancet Infect Dis. 2016;16(2):161-8.  https://doi.org/10.1016/S1473-3099(15)00424-7  PMID: 26603172 
  6. Li R, Xie M, Zhang J, Yang Z, Liu L, Liu X, et al. Genetic characterization of mcr-1-bearing plasmids to depict molecular mechanisms underlying dissemination of the colistin resistance determinant. J Antimicrob Chemother. 2017;72(2):393-401.  https://doi.org/10.1093/jac/dkw411  PMID: 28073961 
  7. Clinical and Laboratory Standards Institute (CLSI). Performance Standards for Antimicrobial Susceptibility Testing; Twenty-fifth informational supplement. Wayne, PA: CLSI; 2015. CLSI document M100-S25.
  8. Clinical and Laboratory Standards Institute (CLSI). Performance Standards for Antimicrobial Susceptibility Testing; Twenty-sixth informational supplement. Wayne, PA: CLSI; 2016. CLSI document M100-S26.
  9. Dallenne C, Da Costa A, Decré D, Favier C, Arlet G. Development of a set of multiplex PCR assays for the detection of genes encoding important beta-lactamases in Enterobacteriaceae. J Antimicrob Chemother. 2010;65(3):490-5.  https://doi.org/10.1093/jac/dkp498  PMID: 20071363 
  10. Borgia S, Lastovetska O, Richardson D, Eshaghi A, Xiong J, Chung C, et al. Outbreak of carbapenem-resistant enterobacteriaceae containing blaNDM-1, Ontario, Canada. Clin Infect Dis. 2012;55(11):e109-17.  https://doi.org/10.1093/cid/cis737  PMID: 22997214 
  11. Wang X, Chen G, Wu X, Wang L, Cai J, Chan EW, et al. Increased prevalence of carbapenem resistant Enterobacteriaceae in hospital setting due to cross-species transmission of the bla NDM-1 element and clonal spread of progenitor resistant strains. Front Microbiol. 2015;6:595. PMID: 26136735 
  12. Overbeek R, Olson R, Pusch GD, Olsen GJ, Davis JJ, Disz T, et al. The SEED and the Rapid Annotation of microbial genomes using Subsystems Technology (RAST). Nucleic Acids Res. 2014;42(Database issue):D206-14.  https://doi.org/10.1093/nar/gkt1226  PMID: 24293654 
  13. Alikhan NF, Petty NK, Ben Zakour NL, Beatson SA. BLAST Ring Image Generator (BRIG): simple prokaryote genome comparisons. BMC Genomics. 2011;12(1):402.  https://doi.org/10.1186/1471-2164-12-402  PMID: 21824423 
  14. Skov RL, Monnet DL. Plasmid-mediated colistin resistance (mcr-1 gene): three months later, the story unfolds. Euro Surveill. 2016;21(9):30155.  https://doi.org/10.2807/1560-7917.ES.2016.21.9.30155  PMID: 26967914 
  15. Zhi C, Lv L, Yu LF, Doi Y, Liu JH. Dissemination of the mcr-1 colistin resistance gene. Lancet Infect Dis. 2016;16(3):292-3.  https://doi.org/10.1016/S1473-3099(16)00063-3  PMID: 26973307 
  16. Stoesser N, Mathers AJ, Moore CE, Day NP, Crook DW. Colistin resistance gene mcr-1 and pHNSHP45 plasmid in human isolates of Escherichia coli and Klebsiella pneumoniae. Lancet Infect Dis. 2016;16(3):285-6.  https://doi.org/10.1016/S1473-3099(16)00010-4  PMID: 26774239 
  17. Zhang R, Huang Y, Chan EW, Zhou H, Chen S. Dissemination of the mcr-1 colistin resistance gene. Lancet Infect Dis. 2016;16(3):291-2.  https://doi.org/10.1016/S1473-3099(16)00062-1  PMID: 26973306 
  18. Xavier BB, Lammens C, Ruhal R, Kumar-Singh S, Butaye P, Goossens H, et al. Identification of a novel plasmid-mediated colistin-resistance gene, mcr-2, in Escherichia coli, Belgium, June 2016. Euro Surveill. 2016;21(27):30280.  https://doi.org/10.2807/1560-7917.ES.2016.21.27.30280  PMID: 27416987 
  19. Yin W, Li H, Shen Y, Liu Z, Wang S, Shen Z, et al. Novel Plasmid-Mediated Colistin Resistance Gene mcr-3 in Escherichia coli. MBio. 2017;8(3):e00543-17.  https://doi.org/10.1128/mBio.00543-17  PMID: 28655818 
  20. Shen Z, Wang Y, Shen Y, Shen J, Wu C. Early emergence of mcr-1 in Escherichia coli from food-producing animals. Lancet Infect Dis. 2016;16(3):293.  https://doi.org/10.1016/S1473-3099(16)00061-X  PMID: 26973308 
  21. Quesada A, Ugarte-Ruiz M, Iglesias MR, Porrero MC, Martínez R, Florez-Cuadrado D, et al. Detection of plasmid mediated colistin resistance (MCR-1) in Escherichia coli and Salmonella enterica isolated from poultry and swine in Spain. Res Vet Sci. 2016;105:134-5.  https://doi.org/10.1016/j.rvsc.2016.02.003  PMID: 27033921 
  22. Sullivan MJ, Petty NK, Beatson SA. Easyfig: a genome comparison visualizer. Bioinformatics. 2011;27(7):1009-10.  https://doi.org/10.1093/bioinformatics/btr039  PMID: 21278367 
Widespread distribution of mcr-1-bearing bacteria in the ecosystem, 2015 to 2016
<p class="citation"> <span>Citation style for this article:</span> <span class="meta-value authors"> <a href="/search?value1=Kaichao+Chen&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Chen Kaichao</a>, <a href="/search?value1=Edward+Wai-Chi+Chan&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Chan Edward Wai-Chi</a>, <a href="/search?value1=Miaomiao+Xie&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Xie Miaomiao</a>, <a href="/search?value1=Liangwei+Ye&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Ye Liangwei</a>, <a href="/search?value1=Ning+Dong&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Dong Ning</a>, <a href="/search?value1=Sheng+Chen&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Chen Sheng</a></span>. Widespread distribution of mcr-1-bearing bacteria in the ecosystem, 2015 to 2016. <a href="/content/ecdc">Euro Surveill.</a> 2017;22(39):pii=17-00206. <a href="https://doi.org/10.2807/1560-7917.ES.2017.22.39.17-00206" title="doi link" target="_blank">https://doi.org/10.2807/1560-7917.ES.2017.22.39.17-00206</a> <span class="ES_Article_citation"> <span class="generated">Received</span>: 13 Mar 2017;&nbsp;&nbsp; <span class="generated">Accepted</span>: 17 Aug 2017 </span> </p>
/content/10.2807/1560-7917.ES.2017.22.39.17-00206
/content/10.2807/1560-7917.ES.2017.22.39.17-00206
Loading

Data & Media loading...

Submit comment
Close
Comment moderation successfully completed
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error