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Abstract

The aim of this study was to identify and characterise from a unique national collection of 564 strains associated with 140 strong-evidence food-borne outbreaks (FBOs) occurring in France during 2007 to 2014. Starchy food and vegetables were the most frequent food vehicles identified; 747 of 911 human cases occurred in institutional catering contexts. Incubation period was significantly shorter for emetic strains compared with diarrhoeal strains A sub-panel of 149 strains strictly associated to 74 FBOs and selected on Coliphage M13-PCR pattern, was studied for detection of the genes encoding cereulide, diarrhoeic toxins (Nhe, Hbl, CytK1 and CytK2) and haemolysin (HlyII), as well as phylogenetic classification. This clustered the strains into 12 genetic signatures (GSs) highlighting the virulence potential of each strain. GS1 ( genes only) and GS2 (, and ), were the most prevalent GS and may have a large impact on human health as they were present in 28% and 31% of FBOs, respectively. Our study provides a convenient molecular scheme for characterisation of strains responsible for FBOs in order to improve the monitoring and investigation of -induced FBOs, assess emerging clusters and diversity of strains.

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/content/10.2807/1560-7917.ES.2016.21.48.30413
2016-12-01
2024-11-21
http://instance.metastore.ingenta.com/content/10.2807/1560-7917.ES.2016.21.48.30413
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Bacillus cereus-induced food-borne outbreaks in France, 2007 to 2014: epidemiology and genetic characterisation
<p class="citation"> <span>Citation style for this article:</span> <span class="meta-value authors"> <a href="/search?value1=Benjamin+Glasset&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Glasset Benjamin</a>, <a href="/search?value1=Sabine+Herbin&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Herbin Sabine</a>, <a href="/search?value1=Laurent+Guillier&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Guillier Laurent</a>, <a href="/search?value1=Sabrina+Cadel-Six&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Cadel-Six Sabrina</a>, <a href="/search?value1=Marie-L%C3%A9one+Vignaud&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Vignaud Marie-Léone</a>, <a href="/search?value1=Joel+Grout&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Grout Joel</a>, <a href="/search?value1=Sylvie+Pairaud&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Pairaud Sylvie</a>, <a href="/search?value1=Val%C3%A9rie+Michel&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Michel Valérie</a>, <a href="/search?value1=Jacques-Antoine+Hennekinne&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Hennekinne Jacques-Antoine</a>, <a href="/search?value1=Nalini+Ramarao&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Ramarao Nalini</a>, <a href="/search?value1=Anne+Brisabois&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Brisabois Anne</a></span>. Bacillus cereus-induced food-borne outbreaks in France, 2007 to 2014: epidemiology and genetic characterisation. <a href="/content/ecdc">Euro Surveill.</a> 2016;21(48):pii=30413. <a href="https://doi.org/10.2807/1560-7917.ES.2016.21.48.30413" title="doi link" target="_blank">https://doi.org/10.2807/1560-7917.ES.2016.21.48.30413</a> <span class="ES_Article_citation"> <span class="generated">Received</span>: 22 Dec 2015;&nbsp;&nbsp; <span class="generated">Accepted</span>: 16 Jul 2016 </span> </p>
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