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Abstract

A standardised method for determining O157:H7 strain relatedness using whole genome sequencing or virulence gene profiling is not yet established. We sought to assess the capacity of either high-throughput polymerase chain reaction (PCR) of 49 virulence genes, core-genome single nt variants (SNVs) or -mer clustering to discriminate between outbreak-associated and sporadic O157:H7 isolates. Three outbreaks and multiple sporadic isolates from the province of Alberta, Canada were included in the study. Two of the outbreaks occurred concurrently in 2014 and one occurred in 2012. Pulsed-field gel electrophoresis (PFGE) and multilocus variable-number tandem repeat analysis (MLVA) were employed as comparator typing methods. The virulence gene profiles of isolates from the 2012 and 2014 Alberta outbreak events and contemporary sporadic isolates were mostly identical; therefore the set of virulence genes chosen in this study were not discriminatory enough to distinguish between outbreak clusters. Concordant with PFGE and MLVA results, core genome SNV and -mer phylogenies clustered isolates from the 2012 and 2014 outbreaks as distinct events. -mer phylogenies demonstrated increased discriminatory power compared with core SNV phylogenies. Prior to the widespread implementation of whole genome sequencing for routine public health use, issues surrounding cost, technical expertise, software standardisation, and data sharing/comparisons must be addressed.

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2015-11-26
2024-12-19
/content/10.2807/1560-7917.ES.2015.20.47.30073
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The utility of multiple molecular methods including whole genome sequencing as tools to differentiate Escherichia coli O157:H7 outbreaks
<p class="citation"> <span>Citation style for this article:</span> <span class="meta-value authors"> <a href="/search?value1=Byron+M+Berenger&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Berenger Byron M</a>, <a href="/search?value1=Chrystal+Berry&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Berry Chrystal</a>, <a href="/search?value1=Trevor+Peterson&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Peterson Trevor</a>, <a href="/search?value1=Patrick+Fach&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Fach Patrick</a>, <a href="/search?value1=Sabine+Delannoy&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Delannoy Sabine</a>, <a href="/search?value1=Vincent+Li&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Li Vincent</a>, <a href="/search?value1=Lorelee+Tschetter&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Tschetter Lorelee</a>, <a href="/search?value1=Celine+Nadon&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Nadon Celine</a>, <a href="/search?value1=Lance+Honish&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Honish Lance</a>, <a href="/search?value1=Marie+Louie&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Louie Marie</a>, <a href="/search?value1=Linda+Chui&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Chui Linda</a></span>. The utility of multiple molecular methods including whole genome sequencing as tools to differentiate Escherichia coli O157:H7 outbreaks. <a href="/content/ecdc">Euro Surveill.</a> 2015;20(47):pii=30073. <a href="https://doi.org/10.2807/1560-7917.ES.2015.20.47.30073" title="doi link" target="_blank">https://doi.org/10.2807/1560-7917.ES.2015.20.47.30073</a> <span class="ES_Article_citation"> <span class="generated">Received</span>: 05 May 2015;&nbsp;&nbsp; <span class="generated">Accepted</span>: 19 Oct 2015 </span> </p>
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