Specific serology for emerging human coronaviruses by protein microarray

C Reusken; H Mou; G J Godeke; L van der Hoek; B Meyer; M A Müller; B Haagmans; R de Sousa; N Schuurman; U Dittmer; P Rottier; A Osterhaus; C Drosten; B J Bosch; M Koopmans

doi:10.2807/1560-7917.ES2013.18.14.20441

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Specific serology for emerging human coronaviruses by protein microarray
C Reusken^1,2 , H Mou^1,3 , G J Godeke^1,2 , L van der Hoek⁴ , B Meyer⁵ , M A Müller⁵ , B Haagmans⁶ , R de Sousa² , N Schuurman³ , U Dittmer⁷ , P Rottier³ , A Osterhaus⁶ , C Drosten⁵ , B J Bosch³ , M Koopmans^2,6
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Affiliations: ¹ These authors contributed equally to this work ² Centre for Infectious Disease Control, Division Virology, National Institute for Public Health and the Environment, Bilthoven, the Netherlands ³ Department of Infectious Diseases and Immunology, Utrecht University, Faculty of Veterinary Medicine, Utrecht, the Netherlands ⁴ Laboratory of Experimental Virology, Department of Medical Microbiology, Center for Infection and Immunity Amsterdam, Academic Medical Center, University of Amsterdam, Amsterdam, the Netherlands ⁵ Institute of Virology, University of Bonn Medical Centre, Bonn, Germany ⁶ Department of Viroscience, Erasmus Medical Centre, Rotterdam, the Netherlands ⁷ Institute for Virology, University Hospital Essen, University of Duisburg-Essen, Essen, Germany

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C Reusken
chantal.reusken rivm.nl
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Citation style for this article: Reusken C, Mou H, Godeke G J, van der Hoek L, Meyer B, Müller M A, Haagmans B, de Sousa R, Schuurman N, Dittmer U, Rottier P, Osterhaus A, Drosten C, Bosch B J, Koopmans M. Specific serology for emerging human coronaviruses by protein microarray. Euro Surveill. 2013;18(14):pii=20441. https://doi.org/10.2807/1560-7917.ES2013.18.14.20441 Received: 29 Mar 2013

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Abstract

We present a serological assay for the specific detection of IgM and IgG antibodies against the emerging human coronavirus hCoV-EMC and the SARS-CoV based on protein microarray technology. The assay uses the S1 receptor-binding subunit of the spike protein of hCoV-EMC and SARS-CoV as antigens. The assay has been validated extensively using putative cross-reacting sera of patient cohorts exposed to the four common hCoVs and sera from convalescent patients infected with hCoV-EMC or SARS-CoV.