1887
  1. Home
  2. Eurosurveillance
  3. Volume 22, Issue 28, 13/Jul/2017
  4. Article
Rapid communication Open Access
Like 0
Download

Abstract

We evaluated a widely used culture-based method for detection of livestock-associated meticillin-resistant (LA-MRSA) in samples collected from pigs and the environment inside pig stables in Denmark and Norway. Selective enrichment in tryptic soy broth containing cefoxitin and aztreonam led to a high ratio of false-negative results (26%; 57/221). On this basis, we recommend reconsidering the use of selective enrichment for detection of LA-MRSA in animal and environmental samples.

© Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Loading

Article metrics loading...

/content/10.2807/1560-7917.ES.2017.22.28.30573
2017-07-13
2024-04-20
http://instance.metastore.ingenta.com/content/10.2807/1560-7917.ES.2017.22.28.30573
Loading
Loading full text...

Full text loading...

/deliver/fulltext/eurosurveillance/22/28/eurosurv-22-28-2.html?itemId=/content/10.2807/1560-7917.ES.2017.22.28.30573&mimeType=html&fmt=ahah

References

  1. Böcher S, Smyth R, Kahlmeter G, Kerremans J, Vos MC, Skov R. Evaluation of four selective agars and two enrichment broths in screening for methicillin-resistant Staphylococcus aureus. J Clin Microbiol. 2008;46(9):3136-8.  https://doi.org/10.1128/JCM.00478-08  PMID: 18632905 
  2. Resultaterne af screening for husdyr-MRSA i svin i 2016. [Screening results for livestock MRSA in pigs in 2016]. Glostrup: Danish Veterinary and Food Administration; 2017. Danish. Available from: https://www.foedevarestyrelsen.dk/Nyheder/Aktuelt/Documents/MRSA%20ekspertgruppe%20-%20resultatene%20forekomst%20af%20husdyr-MRSA%20i%20svin%202016.pdf
  3. The surveillance programme for methicillin resistant Staphylococcus aureus in pigs in Norway 2014. Oslo: Norwegian Veterinary Institute; 2014. Available from: https://www.vetinst.no/overvaking/mrsa-svin/_/attachment/download/47f79404-fecc-401a-82ec-1244e7ecea36:24bc26577546462baefddc8f8122b0910433dddc/2014_methicillin%20resistant%20Staphylococcus%20aureus%20in%20pigs%20in%20Norway%202014.pdf
  4. The surveillance programme for methicillin resistant Staphylococcus aureus in pigs in Norway 2015. Annual report. Oslo: Norwegian Veterinary Institute; 2016. Available from: https://www.vetinst.no/overvaking/mrsa-svin/_/attachment/download/d6c9a586-cb5e-4ab3-8bd2-5874a6910e7b:a636e238f903d737fcbe0a92448ddd82d916743c/2015_methicillin%20resistant%20Staphylococcus%20aureus%20in%20%20pigs%20in%20Norway%202015.pdf
  5. European Union Reference Laboratory for Antimicrobial Resistance. Protocol for PCR amplification of mecA, mecC (mecALGA251), spa and pvl. Lyngby: Technical University of Denmark; 2012. Available from: http://www.eurl-ar.eu/data/images/protocols/pcr_spa_pvl_meca_mecc_sept12.pdf
  6. Biswas B. Assessing agreement for diagnostic devices. US Food and Drug Administration/Industry Statistics Workshop, 28-29 Sep 2006. Available from: http://www.amstat.org/meetings/fdaworkshop/presentations/2006/Assessing%20Agreement%20for%20diagnostic%20tests%202006.ppt
  7. van Cleef BA, Monnet DL, Voss A, Krziwanek K, Allerberger F, Struelens M, et al. Livestock-associated methicillin-resistant Staphylococcus aureus in humans, Europe. Emerg Infect Dis. 2011;17(3):502-5.  https://doi.org/10.3201/eid1703.101036  PMID: 21392444 
  8. European Food Safety Authority (EFSA). Analysis of the baseline survey on the prevalence of methicillin-resistant Staphylococcus aureus (MRSA) in holdings with breeding pigs, in the EU, 2008. Part A: MRSA prevalence estimates. EFSA J. 2009;7(11):1376.
  9. Danish Integrated Antimicrobial Resistance Monitoring and Research Programme. DANMAP 2015: use of antimicrobial agents and occurrence of antimicrobial resistance in bacteria from food animals, food and humans in Denmark. Copenhagen: Statens Serum Institut; 2016. Available from: http://www.danmap.org/~/media/Projekt%20sites/Danmap/DANMAP%20reports/DANMAP%20%202015/DANMAP%202015.ashx
  10. Larsen J, Petersen A, Sørum M, Stegger M, van Alphen L, Valentiner-Branth P, et al. Meticillin-resistant Staphylococcus aureus CC398 is an increasing cause of disease in people with no livestock contact in Denmark, 1999 to 2011. Euro Surveill. 2015;20(37):30021.  https://doi.org/10.2807/1560-7917.ES.2015.20.37.30021  PMID: 26535590 
  11. Larsen J, Petersen A, Larsen AR, Sieber RN, Stegger M, Koch A, et al. Emergence of livestock-associated methicillin-resistant Staphylococcus aureus bloodstream infections in Denmark. Clin Infect Dis. 2017; (Forthcoming).  https://doi.org/10.1093/cid/cix504  PMID: 28575216 
  12. The surveillance program for methicillin resistant Staphylococcus aureus in pigs in Norway 2016. Oslo: Norwegian veterinary Institute; 2017. Available from: https://www.vetinst.no/overvaking/mrsa-svin/_/attachment/download/898d60ff-9511-4a03-9cee-a1481929aaf9:ad5132e40a800cbc45313aa10b8f476115d935d3/2017_OK_MRSA%20Svin_Report%202016.pdf
  13. Grøntvedt CA, Elstrøm P, Stegger M, Skov RL, Skytt Andersen P, Larssen KW, et al. Methicillin-resistant Staphylococcus aureus CC398 in humans and pigs in Norway: a "one health" perspective on introduction and transmission. Clin Infect Dis. 2016;63(11):1431-8.  https://doi.org/10.1093/cid/ciw552  PMID: 27516381 
  14. Krause M, Cavaco L. Isolation of MRSA from dust samples. Lyngby: Technical University of Denmark; 2009. Available from: http://www.eurl-ar.eu/data/images/tc_april-2009/3-final%20mrsa%20protocol.pdf
  15. European Union Reference Laboratory for Antimicrobial Resistance. Protocol for screening for MRSA. Lyngby: Technical University of Denmark; 2009. Available from: http://www.eurl-ar.eu/data/images/tc_april-2009/4-protocol%20for%20screening%20mrsa.pdf
  16. European Food Safety Authority (EFSA). Technical specifications on the harmonised monitoring and reporting of antimicrobial resistance in methicillin-resistant Staphylococcus aureus in food-producing animals and food. EFSA J. 2012;10(10):2897.  https://doi.org/10.2903/j.efsa.2012.2897 
  17. Larsen J, Stegger M, Andersen PS, Petersen A, Larsen AR, Westh H, et al. Evidence for human adaptation and foodborne transmission of livestock-associated methicillin-resistant Staphylococcus aureus. Clin Infect Dis. 2016;63(10):1349-52.  https://doi.org/10.1093/cid/ciw532  PMID: 27655995 
  18. Nemeghaire S, Roelandt S, Argudín MA, Haesebrouck F, Butaye P. Characterization of methicillin-resistant Staphylococcus aureus from healthy carrier chickens. Avian Pathol. 2013;42(4):342-6.  https://doi.org/10.1080/03079457.2013.805183  PMID: 23777220 
  19. Nemeghaire S, Argudín MA, Haesebrouck F, Butaye P. Epidemiology and molecular characterization of methicillin-resistant Staphylococcus aureus nasal carriage isolates from bovines. BMC Vet Res. 2014;10(1):153.  https://doi.org/10.1186/1746-6148-10-153  PMID: 25011427 
Evaluation of a widely used culture-based method for detection of livestock-associated meticillin-resistant Staphylococcus aureus (MRSA), Denmark and Norway, 2014 to 2016
<p class="citation"> <span>Citation style for this article:</span> <span class="meta-value authors"> <a href="/search?value1=Jesper+Larsen&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Larsen Jesper</a>, <a href="/search?value1=Marianne+Sunde&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Sunde Marianne</a>, <a href="/search?value1=Md+Zohorul+Islam&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Islam Md Zohorul</a>, <a href="/search?value1=Anne+Margrete+Urdahl&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Urdahl Anne Margrete</a>, <a href="/search?value1=Aina+Steihaug+Barstad&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Barstad Aina Steihaug</a>, <a href="/search?value1=Anders+Rhod+Larsen&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Larsen Anders Rhod</a>, <a href="/search?value1=Carl+Andreas+Gr%C3%B8ntvedt&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Grøntvedt Carl Andreas</a>, <a href="/search?value1=%C3%98ystein+Angen&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Angen Øystein</a></span>. Evaluation of a widely used culture-based method for detection of livestock-associated meticillin-resistant Staphylococcus aureus (MRSA), Denmark and Norway, 2014 to 2016. <a href="/content/ecdc">Euro Surveill.</a> 2017;22(28):pii=30573. <a href="https://doi.org/10.2807/1560-7917.ES.2017.22.28.30573" title="doi link" target="_blank">https://doi.org/10.2807/1560-7917.ES.2017.22.28.30573</a> <span class="ES_Article_citation"> <span class="generated">Received</span>: 13 Jun 2017;&nbsp;&nbsp; <span class="generated">Accepted</span>: 11 Jul 2017 </span> </p>
/content/10.2807/1560-7917.ES.2017.22.28.30573
/content/10.2807/1560-7917.ES.2017.22.28.30573
Loading

Data & Media loading...

Submit comment
Close
Comment moderation successfully completed
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error